A5022086765- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Nanoparticle-Based Drug Delivery
- IL-33, ST2, and ILC Pathways
- Nanoplatforms for cancer theranostics
- Retinal Diseases and Treatments
- RNA Interference and Gene Delivery
- Immune cells in cancer
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Inflammation biomarkers and pathways
- Immune Cell Function and Interaction
- Photoacoustic and Ultrasonic Imaging
- Chemical Reactions and Isotopes
- Cancer Diagnosis and Treatment
- Metastasis and carcinoma case studies
- Nanopore and Nanochannel Transport Studies
- Neurological Disease Mechanisms and Treatments
- DNA Repair Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Alzheimer's disease research and treatments
- Advanced Electron Microscopy Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- Mesenchymal stem cell research
- Retinal Development and Disorders
IRCCS Humanitas Research Hospital
2016-2025
Neuroscience Institute
2016-2022
Humanitas University
2018-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2020
University of Milan
2015-2020
Neuronal circuit assembly requires the fine balance between synapse formation and elimination. Microglia, through elimination of supernumerary synapses, have an established role in this process. While microglial receptor TREM2 soluble complement proteins C1q C3 are recognized as key players, neuronal molecular components that specify synapses to be eliminated still undefined. Here, we show exposed phosphatidylserine (PS) represents a "eat-me" signal involved microglial-mediated pruning. In...
Bone marrow Mesenchymal Stem Cells (BM-MSCs), due to their strong protective and anti-inflammatory abilities, have been widely investigated in the context of several diseases for possible therapeutic role, based on release a highly proactive secretome composed soluble factors Extracellular Vesicles (EVs). BM-MSC-EVs, particular, convey many beneficial features parental cells, including direct indirect β-amyloid degrading-activities, immunoregulatory neurotrophic abilities. Therefore, EVs...
Glioblastoma multiforme (GBM) is the most aggressive form of glioma, with life expectancy around 2 years after diagnosis, due to recidivism and blood-brain barrier (BBB) limiting amount drugs which reach residual malignant cells, thus contributing failure chemotherapies. To bypass obstacles imposed by BBB, we investigated use nanotechnologies combined radiotherapy, as a potential therapeutic strategy for GBM. We used poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PNP) conjugated...
Abstract Background Although invasiveness is one of the major determinants poor glioblastoma (GBM) outcome, mechanisms GBM invasion are only partially understood. Among intrinsic and environmental processes promoting cell-to-cell interaction processes, eventually driving invasion, we focused on pro-invasive role played by Extracellular Vesicles (EVs), a heterogeneous group cell-released membranous structures containing various bioactive cargoes, which can be transferred from donor to...
Substantial data indicate that amyloid-β (Aβ), the major component of senile plaques, plays a central role in Alzheimer's Disease and indeed assembly naturally occurring amyloid peptides into cytotoxic aggregates is linked to disease pathogenesis. Although Aβ42 highly aggregating form Aβ, co-occurrence shorter Aβ might affect aggregation potential pool. In this study we aimed assess whether structural behavior human peptide inside brain influenced by concomitant presence N-terminal fragments...
The capacity to guarantee the proper excitatory/inhibitory balance is one of most critical steps during early development responsible for correct brain organization, function, and plasticity. GABAergic neurons guide this process leading right structural circuitry, neuronal firing. Here, we identified ataxia telangiectasia mutated (ATM), a serine/threonine protein kinase linked DNA damage response, as crucial in regulating neurotransmission. We found that reduced levels ATM hippocampal...
Abstract Neuronal circuits assembly requires the fine equilibrium between synapse formation and elimination. Microglia, through elimination of supernumerary synapses, have an established role in this process. While microglial receptor TREM2 soluble complement proteins C1q C3 are recognized key players process, neuronal molecular components that tag synapses to be eliminated still undefined. Here we show exposed phosphatidylserine (PS) represents a ‘eat-me’ signal enabling microglial-mediated...
The radio- and chemo-resistance of glioblastoma stem-like cells (GSCs), together with their innate tumor-initiating aptitude, make this cell population a crucial target for effective therapies. However, targeting GSCs is hardly difficult complex, due to the presence blood-brain barrier (BBB) infiltrative nature arousing dispersion within brain parenchyma.Liposomes (LIPs), surface-decorated an Apolipoprotein E-modified peptide (mApoE) enable BBB crossing, were loaded doxorubicin (DOXO), as...
Journal Article mApoE-Functionalized Nanoliposomes Delivering Doxorubicin and Ultrasmall Superparamagnetic Iron Oxide to Glioblastoma Cells Characterized by TEM Confocal Microscopy Get access Simona Rodighiero, Rodighiero Molecular Cellular Imaging Platform, Fondazione Filarete, Milan, Italy Search for other works this author on: Oxford Academic Google Scholar Maria Gregori, Gregori Department of Health Sciences, University Milano-Bicocca, Maura Francolini, Francolini ItalyDepartment Medical...
Abstract To promote cell growth, invasion and therapy resistance, glioblastoma (GBM) makes use of different communication routes with the neighbor environment which include Extracellular Vesicles (EVs). EVs are a heterogeneous group cell-released membranous structures, contain wide mixture active molecules. Each type secretes unique combination EV subpopulations that vary in size, content function. In GBM, subfraction small-EVs derived from multivesicular bodies, also referred as exosomes...