A5069428450- Hemoglobin structure and function
- Heme Oxygenase-1 and Carbon Monoxide
- Metal-Catalyzed Oxygenation Mechanisms
- Porphyrin Metabolism and Disorders
- Pharmacogenetics and Drug Metabolism
- Porphyrin and Phthalocyanine Chemistry
- Cancer Treatment and Pharmacology
- Asymmetric Hydrogenation and Catalysis
- Biochemical and Structural Characterization
- Mass Spectrometry Techniques and Applications
- Crystallization and Solubility Studies
- Antimicrobial Peptides and Activities
- Cyclopropane Reaction Mechanisms
- X-ray Diffraction in Crystallography
- Chemical Synthesis and Analysis
- Crystallography and molecular interactions
- Toxin Mechanisms and Immunotoxins
- Microbial Natural Products and Biosynthesis
- Bacteriophages and microbial interactions
- Synthetic Organic Chemistry Methods
- Nanopore and Nanochannel Transport Studies
- Protein Structure and Dynamics
- Neonatal Health and Biochemistry
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
Nagoya University
2017-2024
RIKEN Center for Sustainable Resource Science
2022-2023
Graduate School USA
2019
Biological methane oxidation is a highly desirable method for the conversion of natural gas into liquid to meet increasing demand fuel and chemical feedstock as well reducing potent greenhouse effects emissions. Because hemoenzymes that can catalyze methanol have not been found, it has long considered hemoenzymes, including cytochrome P450s (P450s), cannot oxidative methane. Herein, we report catalytic by wild-type P450BM3, without any mutagenesis, in presence chemically evolved dummy...
To survive in the iron-devoid environment of their host, pathogenic bacteria have devised multifarious cunning tactics such as evolving intricate heme transport systems to pirate extracellular heme. Yet, potential antimicrobial targets has not been explored. Herein we developed a strategy deliver antimicrobials by exploiting acquisition system protein A (HasA) Pseudomonas aeruginosa. We demonstrated that, analogous uptake, HasA can specifically traffic an antimicrobial, gallium...
Stereoselectivity of cyclopropanation styrene catalysed by cytochrome P450BM3 is enhanced in the presence decoy molecules.
Bulky metallo-tetraphenylporphycene was successfully incorporated into haemophore HasA which have flexible loops surrounding heme-binding site.
Abstract Iron(III)‐5,15‐diphenylporphyrin and several derivatives were accommodated by HasA, a heme acquisition protein secreted Pseudomonas aeruginosa , despite possessing bulky substituents at the meso position of porphyrin. Crystal structure analysis revealed that two phenyl groups positions porphyrin extend outside HasA. It was shown growth P. inhibited in presence HasA coordinating synthetic porphyrins under iron‐limiting conditions, greatly affects inhibition efficiency.
Abstract Iron(III)‐5,15‐diphenylporphyrin and several derivatives were accommodated by HasA, a heme acquisition protein secreted Pseudomonas aeruginosa , despite possessing bulky substituents at the meso position of porphyrin. Crystal structure analysis revealed that two phenyl groups positions porphyrin extend outside HasA. It was shown growth P. inhibited in presence HasA coordinating synthetic porphyrins under iron‐limiting conditions, greatly affects inhibition efficiency.
Tetraphenylporphyrin (TPP) is a symmetrically substituted synthetic porphyrin whose properties can be readily modified, providing it with significant advantages over naturally occurring porphyrins. Herein, we report the first example of stable complex between native biomolecule, haemoprotein HasA, and TPP as well its derivatives. The X-ray crystal structures nine different HasA-TPP complexes were solved at high resolutions. HasA capturing derivatives was also demonstrated to inhibit growth...
Abstract Catching the structure of cytochrome P450 enzymes in flagrante is crucial for development biocatalysts, as most structures collected are found trapped a precatalytic conformation. At heart catalysis lies Cpd I, short‐lived, highly reactive intermediate, whose recalcitrant nature has thwarted attempts at capturing catalytically relevant poses P450s. We report crystal P450BM3 mimicking state precise moment preceding epoxidation, which perfect agreement with experimentally observed...
Construction of a bevel-gear-like protein assembly using synthetic porphyrin with defined flexibility.
Abstract Das Erfassen der Struktur von Zytochrom‐P450‐Enzymen in flagranti ist für die Entwicklung P450‐Biokatalysatoren entscheidender Bedeutung, da meisten bisher erfassten Strukturen einer präkatalytischen Konformation gefangen sind. Herzstück P450‐Katalyse Verbindung I (Vbd I), ein kurzlebiges, hochreaktives Zwischenprodukt, dessen widerspenstige Art Mehrheit Versuche vereitelt hat, katalytisch bedeutsame Konformationen P450 zu erfassen. Wir zeigen Kristallstruktur P450BM3, den Zustand...
Tetraphenylporphyrin (TPP) is a synthetic porphyrin whose properties can be readily modified, endowing it with significant benefits over naturally occurring porphyrins. Yet, their insolubility in water and/or steric bulk have rendered them incompatible biological systems. Herein, we report the first example of native biomolecule capturing TPP as well its derivatives. The haemoprotein HasA, secreted by certain pathogens to scavenge haem from hosts, capture various metal- and meso-substituted...
Enzymes. In their Research Article (e202215706), Hiroshi Sugimoto, Osami Shoji et al. report the crystal structure of cytochrome P450BM3 mimicking state enzyme in moment preceding epoxidation.
Das Hämbindeprotein HasA aus P. aeruginosa kann Eisen(III)-5,15-diphenylporphyrin und mehrere Derivate aufnehmen, auch wenn sie sperrige Substituenten in der meso-Position des Porphyrins tragen. O. Shoji et al. präsentieren ihrer Zuschrift auf S. 15481 Kristallstrukturen dieser künstlichen HasAs weisen nach, dass die wachstumsinhibierende Wirkung gegen je nach Struktur eingefangenen Metallkomplexes variiert.
Tetraphenylporphyrin (TPP) is a synthetic porphyrin whose properties can be readily modified, endowing it with significant benefits over naturally occurring porphyrins. Yet, their insolubility in water and/or steric bulk have rendered them incompatible biological systems. Herein, we report the first example of native biomolecule capturing TPP as well its derivatives. The haemoprotein HasA, secreted by certain pathogens to scavenge haem from hosts, capture various metal- and meso-substituted...