A5052180287- Neuroscience and Neuropharmacology Research
- Nanoparticle-Based Drug Delivery
- Iron Metabolism and Disorders
- Trace Elements in Health
- Nanoparticles: synthesis and applications
- Mitochondrial Function and Pathology
- Characterization and Applications of Magnetic Nanoparticles
- Neuroinflammation and Neurodegeneration Mechanisms
- Epilepsy research and treatment
- Olfactory and Sensory Function Studies
- Barrier Structure and Function Studies
- Heavy Metal Exposure and Toxicity
- Graphene and Nanomaterials Applications
- Sulfur Compounds in Biology
- Inflammatory mediators and NSAID effects
- PI3K/AKT/mTOR signaling in cancer
- Iron oxide chemistry and applications
- Indoor Air Quality and Microbial Exposure
- Photoreceptor and optogenetics research
- Neurological Complications and Syndromes
- Cell Image Analysis Techniques
- Ion channel regulation and function
- Sociology and Education Studies
- Pancreatic function and diabetes
- Education Methods and Technologies
University of Copenhagen
2014-2019
University of Bremen
2007-2014
Centre for Sustainable Energy
2012
Université de Montréal
2011
Constructor University
2007
Uppsala University
2007
The protein parkin, encoded by the PARK2 gene, is vital for mitochondrial homeostasis, and although it has been implicated in Parkinson's disease (PD), mechanisms remain unclear. We have applied mass spectrometry-based proteomics to investigate effects of parkin dysfunction on proteome human isogenic induced pluripotent stem cell (iPSC)-derived neurons with without knockout (KO). proteomic analysis quantified nearly 60% all proteins, 119 which were dysregulated KO. changes indicated...
Magnetic iron oxide nanoparticles (Fe-NP) are currently considered for various diagnostic and therapeutic applications in the brain. However, little is known on accumulation biocompatibility of such particles brain cells. We have synthesized characterized dimercaptosuccinic acid (DMSA) coated Fe-NP investigated their uptake by cultured astrocytes. DMSA-coated that were dispersed physiological medium had an average hydrodynamic diameter about 60 nm. Incubation astrocytes with these caused a...
The enzyme glutamate dehydrogenase (GDH; Glud1) catalyzes the (reversible) oxidative deamination of to α-ketoglutarate accompanied by a reduction NAD + NADH. GDH connects amino acid, carbohydrate, neurotransmitter and energy metabolism. Glutamine is precursor used neurons sustain pool glutamate, but glutamine also vividly oxidized for support This study investigates role in neuronal metabolism employing Cns- Glud1 −/− mouse, lacking brain (GDH KO) metabolic mapping using 13 C-labelled...
The glutamate transporter GLT-1 is highly expressed in astrocytes but also neurons, primarily axon terminals. We generated a conditional neuronal KO using synapsin 1-Cre (synGLT-1 KO) to elucidate the metabolic functions of here focusing on cerebral cortex. Both synaptosomal uptake studies and electron microscopic immunocytochemistry demonstrated knockdown cortex synGLT-1 mice. Aspartate content was significantly reduced cortical extracts as well synaptosomes from compared with control...
Magnetic iron oxide nanoparticles are considered for various diagnostic and therapeutic applications in brain including their use as contrast agent magnetic resonance imaging or tool drug delivery. However, little is known so far on the consequences of a treatment cells with such nanoparticles. In order to study biocompatibility accumulation from particles cells, we have used astrocyte-rich primary cultures model system. Iron were chemically synthesized yield about 80% regarding content....
Formaldehyde is endogenously produced in the human body and brain levels of this compound are elevated neurodegenerative conditions. Although toxic potential an excess formaldehyde has been studied, little known on molecular mechanisms underlying its neurotoxicity as well ability neurons to metabolize formaldehyde. To address these topics, we have used cerebellar granule neuron cultures model system. These express mRNAs various enzymes that involved metabolism were remarkably resistant...
A new class of lipids, containing the closo-dodecaborate cluster, has been synthesized. Two S-(N, N-(2-dimyristoyloxyethyl)acetamido)thioundecahydro-closo-dodecaborate (2-) (B-6−14) and N-(2-dipalmitoyloxyethyl)acetamido)thioundecahydro-closo-dodecaborate (B-6−16) are described. Both them have a double-tailed lipophilic part headgroup carrying two negative charges. Differential scanning calorimetry shows that B-6−14 B-6−16 bilayers main phase transition temperatures 18.8 37.9 °C,...
Metformin is an antidiabetic drug that used daily by millions of patients worldwide. able to cross the blood‐brain barrier and has recently been shown increase glucose consumption lactate release in cultured astrocytes. However, potential effects metformin on mitochondrial tricarboxylic acid (TCA) cycle metabolism astrocytes are unknown. We investigated this mapping 13 C labeling TCA intermediates corresponding amino acids after incubation primary rat with [U‐ C]glucose. The presence did not...
Iron oxide nanoparticles (IONP) are currently being studied as green magnet resonance imaging (MRI) contrast agents. They also used in huge quantities for environmental remediation and water treatment purposes, although very little is known on the consequences of such applications organisms ecosystems. In order to address these questions, we synthesised polyvinylpyrrolidone-coated IONP, characterised particle dispersion various media investigated an IONP exposure using array biochemical...
To clarify discrepancies in the literature on adverse effects of hydrogen peroxide neurons, this study investigated application to cultured cerebellar granule neurons with six assays frequently used test for viability. Cultured efficiently cleared exogenous H 2 O . Although viability was not affected by exposure 10 µM peroxide, an higher concentrations rapidly lowered, within 15 min, cellular 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltertrazolium bromide (MTT) reduction capacity 53% ± 1% (100...
Abstract Magnetic iron oxide nanoparticles (Fe‐NP) are considered for various applications in the brain. However, little is known so far on uptake and metabolism of such brain cells. Since astrocytes strategically localized between capillaries neurons, particular interest concerning fate Using astrocyte‐rich primary cultures as model system we have investigated accumulation citrate‐coated Fe‐NP by astrocytes. Viable cultured accumulate from a time‐, concentration‐, temperature‐dependent...