A5051607279- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Hydrogels: synthesis, properties, applications
- Electrowetting and Microfluidic Technologies
- Pickering emulsions and particle stabilization
- Blood transfusion and management
- Electrohydrodynamics and Fluid Dynamics
- Electrospun Nanofibers in Biomedical Applications
- Periodontal Regeneration and Treatments
- Platelet Disorders and Treatments
- Additive Manufacturing and 3D Printing Technologies
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- Electrostatics and Colloid Interactions
- Blood donation and transfusion practices
- Nanofabrication and Lithography Techniques
- Blood groups and transfusion
Toray Industries, Inc. (Japan)
2019
Toray (United States)
2015
Chiba University
2011-2015
Graduate School USA
2015
Hydrogel materials with microscale heterogeneity are of great interest in the effort to spatially control cellular microenvironments tissue engineering applications. Here we present a microfluidic system continuously synthesize chemically and physically anisotropic Ca–alginate hydrogel microfibers enabling guidance cell proliferation form linear colonies intracellular networks. The gelation process involves 2 critical steps obtain alginate using axisymmetric microchannels uniform depth:...
Cell-sized, highly condensed collagen microparticles were produced, which utilized to fabricate composite multicellular spheroids of primary hepatocytes.
Selection of particles or cells specific shapes from a complex mixture is an essential procedure for various biological and industrial applications, including synchronization the cell cycle, classification environmental bacteria, elimination aggregates synthesized particles. Here, we investigate separation behaviors nonspherical spherical particles/cells in hydrodynamic filtration (HDF) scheme, which was previously developed continuous size-dependent particle/cell separation. Nonspherical...
In this study, a microfluidic process is proposed for preparing monodisperse micrometer-sized hydrogel beads. This utilizes non-equilibrium aqueous droplets formed in polar organic solvent. The water-in-oil of the precursor rapidly shrunk owing to dissolution water molecules into continuous phase. shrunken and condensed were then gelled, resulting formation microbeads with sizes significantly smaller than initial droplet size. study employed methyl acetate as solvent, which can dissolve at...
This study presents a simple but highly versatile method of fabricating picoliter-volume hydrogel patterns on poly(dimethylsiloxane) (PDMS) substrates. Hydrophilic regions were prepared hydrophobic PDMS plates by trapping and melting functional polymer particles performing subsequent reactions with partially oxidized dextran. Small aliquots gelation solution selectively trapped the hydrophilic areas dipping process that was utilized to make thin in situ sol solution. Using this process, we...
A simple microfluidic process for producing yarn-ball-shaped hydrogel microbeads was devised. The mechanism employed parallel flows of aqueous solutions a precursor, gelation agent, and buffer to generate incompletely gelled alginate microfibers. Water-in-oil droplets were generated simultaneously fragment the fibers fold fragments into yarn-ball-like shape. Alginate beads with an average outer diameter ∼200 μm fiber width 10–30 fabricated, which had relatively large void volume high...
We developed a hollow-fibre column system specifically adapted to prepare washed platelet concentrates (WPCs). This study was performed evaluate the efficacy of for preparing WPCs.First, percentages (PLT) recovery and remaining plasma proteins were calculated by determining PLT count, volume protein levels in both prewash postwash. Secondly, PLTs unwashed control stored 5 days, changes during this 5-day storage vitro characteristics determined.The effectively removed >98% (PCs), 97% on an...
We present a manipulation technique of cells and cell spheroids by utilizing cell-size collagen hydrogel microbeads. Collagen beads were prepared using non-equilibrium microfluidic W/O droplets containing molecules, formed in continuous phase water-soluble organic solvent. Monodisperse with sizes smaller than 20 μm produced, their morphologies controlled changing the flow rates types adding cross-linking reagents. As applications to spheroid manipulation, magnetic nanoparticles produced then...
Although HLA-eliminated platelets can facilitate transfusions to patients possessing HLA antibodies, no such products are currently available commercially perhaps because the platelet collection rate is not yet economically viable. We have improved this process' efficiency by employing a hollow-fibre system at last step of production process after an acid and reaction buffer been washed out conventionally centrifugation.HLA-eliminated were prepared via four distinct steps: chilled on ice,...
In this report, we present two processes to fabricate unique hydrogel beads, (1) yarn-ball-shape beads and (2) extremely-small utilizing non-equilibrium microfluidics. First, prepare the microbeads, an incompletely-gelled Ca-alginate fiber in a microchannel was cut into pieces incorporated water-in-oil droplets. Then, W/O droplets, composed of water containing molecules as disperse phase water-soluble organic solvent continuous phase, were used extremely small (Φ = ~10 μm). As application,...
Here we present microfluidic systems to produce micrometer-sized particles made of proteins, using monodisperse droplets in a non-equilibrium state. Droplets an aqueous solution protein were formed the continuous phase water-dissolving polar organic solvent, and then shrunk because dissolution water molecules. Protein molecules precipitated, stable microparticles obtained after crosslinking reaction. We spherical/non-spherical several types examined factors affecting particle morphology. In...
Micronozzle array-combined microfluidic devices have been developed to form anisotropically-patterned hydrogel fibers composed of soft and solid regions, which are highly useful linear cell colonies neurite networks. The patterns the vertical micronozzles determine cross-sectional compositions Ca-alginate microfibers, with widths fiber regions 60~100 10~20 μm, respectively. Neuron-like cells (PC12 cells) were inoculated in region fiber, showed unprecedented behaviors colony formation guided...