A5058630840- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Electrohydrodynamics and Fluid Dynamics
- Electrowetting and Microfluidic Technologies
- Electrospun Nanofibers in Biomedical Applications
- Additive Manufacturing and 3D Printing Technologies
- Tissue Engineering and Regenerative Medicine
- Hydrogels: synthesis, properties, applications
- Nanopore and Nanochannel Transport Studies
- Biosensors and Analytical Detection
- Liver physiology and pathology
- Virus-based gene therapy research
- Bone Tissue Engineering Materials
- Prostate Cancer Diagnosis and Treatment
- Pickering emulsions and particle stabilization
- Viral Infectious Diseases and Gene Expression in Insects
- Electrostatics and Colloid Interactions
- Herpesvirus Infections and Treatments
- Cancer Cells and Metastasis
- Nanofabrication and Lithography Techniques
- Pancreatic function and diabetes
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
Mie Chuo Medical Center
2024-2025
Chiba University
2016-2025
Kyoto University
1994-2020
Graduate School USA
2015-2019
National Institute of Advanced Industrial Science and Technology
2013
Tokyo Women's Medical University
2006-2010
Massachusetts Institute of Technology
2009
The University of Tokyo
2001-2008
Osaka Prefecture University
2004-2008
Japan Science and Technology Agency
2007
A concept of "pinched flow fractionation" for the continuous size separation and analysis particles in microfabricated devices has been proposed demonstrated. In this method, suspended liquid were continuously introduced into a microchannel having pinched segment aligned to one sidewall by another without particles. The then separated perpendicularly direction according their sizes spreading profile inside microchannel. Polymer microbeads successfully separated, effects rate channel shapes...
We propose here a new method for continuous concentration and classification of particles in microfluidic devices, named hydrodynamic filtration. When particle is flowing microchannel, the center position cannot be present certain distance from sidewalls, which equal to radius. The proposed utilizes this fact, performed using microchannel having multiple side branch channels. By withdrawing small amount liquid repeatedly main stream through channels, are concentrated aligned onto sidewalls....
A new method for continuous size separation and collection of particles in microfabricated devices, asymmetric pinched flow fractionation (AsPFF), has been proposed demonstrated. This improves the scheme (PFF), which utilizes a laminar profile inside microchannel. In this study, multiple branch channels with different channel dimensions were arranged at end segment, so that rate distributions to each varied, large part liquid was forced go through one (drain channel). system, microchannel...
This paper describes an improved microfluidic device that enables hydrodynamic particle concentration and size-dependent separation to be carried out in a continuous manner. In our previous study, method for filtration sorting of particles was proposed using microchannel having multiple branch points side channels, it applied polymer cells. the current efficiency dramatically by geometrically splitting fluid flow from main stream recombining. With these operations, with diameters larger than...
Hydrogel materials with microscale heterogeneity are of great interest in the effort to spatially control cellular microenvironments tissue engineering applications. Here we present a microfluidic system continuously synthesize chemically and physically anisotropic Ca–alginate hydrogel microfibers enabling guidance cell proliferation form linear colonies intracellular networks. The gelation process involves 2 critical steps obtain alginate using axisymmetric microchannels uniform depth:...
In this report, a microfluidic system is presented for continuous and size-dependent separation of droplets utilizing microscale hydrodynamics. The scheme based on laminar-flow focusing spreading in pinched microchannel, referred to as "pinched flow fractionation (PFF)", which was previously developed the solid particles, such polymer microparticles or cells. By simply introducing emulsion phase into could be achieved without using complicated operations devices. We first examined whether...
Cell-sized, highly condensed collagen microparticles were produced, which utilized to fabricate composite multicellular spheroids of primary hepatocytes.
Hydrodynamic microfluidic platforms have been proven to be useful and versatile for precisely sorting particles/cells based on their physicochemical properties. In this study, we demonstrate that a simple lattice-shaped pattern can work as virtual sieve size-dependent continuous particle sorting. The lattice is composed of two types microchannels ("main channels" "separation channels"). These channels cross each other in perpendicular fashion, are slanted against the macroscopic flow...
Abstract We present a novel microfluidic system in which an aqueous two‐phase laminar flow is stably formed, and the continuous partitioning of relatively large cells can be performed, eliminating influence gravity. In this study, plant cell aggregates whose diameters were 37–96 μm used as model particles. first performed using simple straight microchannel having two inlets outlets examined effects rate phase width on efficiency. Second, by with pinched segment, efficiency was successfully...
A simple microfluidic system has been presented to perform continuous two-parameter cell sorting based on size and surface markers. Immunomagnetic bead-conjugated cells are initially sorted by utilizing the hydrodynamic filtration (HDF) scheme, introduced into individual separation lanes, simultaneously focused onto one sidewall effect. Cells then subjected magnetophoretic in lateral direction, finally they individually recovered through multiple outlet branches. We successfully demonstrated...
Selection of particles or cells specific shapes from a complex mixture is an essential procedure for various biological and industrial applications, including synchronization the cell cycle, classification environmental bacteria, elimination aggregates synthesized particles. Here, we investigate separation behaviors nonspherical spherical particles/cells in hydrodynamic filtration (HDF) scheme, which was previously developed continuous size-dependent particle/cell separation. Nonspherical...
Multilayered microfluidic devices with a micronozzle array structure have been developed to prepare unique hydrogel microfibers highly complex cross-sectional morphologies. Hydrogel precursor solutions different compositions are introduced through vertical micronozzles, united and focused, continuously gelled form fibers multiple regions of physicochemical composition. We prepared alginate diameters 60 ∼ 130 μm 4/8 parallel in the periphery. Neuron-like PC12 cells encapsulated region, which...
Hydrogel encapsulation is a rational approach that facilitates three-dimensional inoculation, arrangement, and culture of living mammalian cells for biomedical applications. However, strategies to form capillary-like conduits in hydrogels remain challenging due low spatial resolution difficulty controlling the location multiple cell types. Herein, we propose highly unique process constructing hydrogel sponges with tailored pore densities using finely fragmented microfibers as sacrificial...
We have developed a microdispenser array made of PDMS, in which number nanoliter-sized droplets can be accurately dispensed and mixed with the aid specific channels under pneumatic pressure. In this system, hydrophobic narrow act as kind valve help structural liquid manipulation. Also, by arranging multiple dispensers parallel, single injection becomes sufficient for preparation aliquots different reactions. designed two kinds microdevices dispensing mixing evaluated their performance...
Abstract Vascular tissues fabricated in vitro are useful tools for studying blood vessel‐related cellular physiologies and constructing relatively large 3D tissues. An efficient strategy fabricating vascular tissue models with multilayered, branched, thick structures through the situ hydrogel formation fluidic channels is proposed. First, an aqueous solution of RGD‐alginate containing smooth muscle cells (SMCs) introduced into channel made agarose hydrogel, forming a cell‐embedding...