A5101799890- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Additive Manufacturing and 3D Printing Technologies
- Mesenchymal stem cell research
- biodegradable polymer synthesis and properties
- Advanced Battery Technologies Research
- Tracheal and airway disorders
- Molecular Biology Techniques and Applications
- Chemical Reactions and Isotopes
- Xenotransplantation and immune response
- Medical and Biological Ozone Research
- Polysaccharides and Plant Cell Walls
- bioluminescence and chemiluminescence research
- Skin and Cellular Biology Research
- Hydrogels: synthesis, properties, applications
- Organ and Tissue Transplantation Research
- Macrophage Migration Inhibitory Factor
- Congenital heart defects research
- Spaceflight effects on biology
- Congenital Diaphragmatic Hernia Studies
- Chemokine receptors and signaling
- 3D Printing in Biomedical Research
- Plant Surface Properties and Treatments
- Collagen: Extraction and Characterization
Ministry of Health of the Russian Federation
2017-2021
Kuban State Medical University
2013-2020
Federal State Budgetary Scientific Institution Research Institute of Medical Primatology
2017-2019
Regenerative Medicine Institute
2017
Kuban State University
2017
Center for Neuroscience and Regenerative Medicine
2017
Sechenov University
2017
Karolinska University Hospital
2013
Karolinska Institutet
2013
The aim of this article is to verify the applicability impulse acoustic microscopy as a tool for evaluating scaffolds in tissue engineering. Decellularized rat diaphragms and nonwoven polymer made from cellulose diacetate, chlorinated polyvinyl chloride, polysulfone were used model objects. Optical microscopy, scanning electron histological research reference methods order realize feasibility method regenerative medicine field. Direct comparison different was carried out. high frequency...
Abstract Development of artificial tissues or organs is one the actual tasks in regenerative medicine that requires observation and evaluation intact volume microstructure tissue engineering products at all stages their formation, from native donor decellularized scaffolds to recipient cell migration matrix. Unfortunately practice, methods vital noninvasive imaging matrixes are absent. In this work, we propose a new approach based on high‐frequency acoustic microscopy for visualization...
Aim. The main aim of our research is to evaluate the process rat lung decellularization and recellularization as initial step tissue-engineered organs creation. Materials methods. Rat was performed by perfusion with detergents enzymes concomitant atmospheric air ventilation through trachea. quality analyzed routine histological immunohistochemical staining techniques, DNA content determined quantitatively spectrophotometer. For static whole organ reseeding a model cells’ behavior mesenchymal...
Automated image analysis methods are highly important for biotechnology research. The authors developed and tested a program the morphometric of photomicrographs sections processed using standard immunohistochemical examination protocols. color deconvolution method used in algorithm was proven to be effective mapping distribution DAB chromogen sample containing multiple dyes. experiment demonstrated that level extracellular matrix proteins could comparatively quantified different groups...
Экспериментальная медицинаT issue engineering is one of the branches regenerative medicine that may offer an alternative treatment option for patients in need organ transplantation.The main point tissue restoring structural and functional intactness tissues organs using bioartificial constructions, which include following [3]:-Cells able to create functioning extracellular matrix.-Suitable biodegradable scaffold (matrix) cells transplantation.
A rapidly growing development of tissue engineering promotes the increasing interest in obtainment various decellularizedtissues and organs. Minimal quality evaluation criteria obtained engineered constructions have been previously specified. In discussionpaper group authors considers morphological methods matrix applied by researchers on model heart decellularization. The analysis modern literature authors’ own researches shown that decellularization has to be complex should consist several...
В рамках экспериментального исследования оптимизирован протокол децеллюляризации пищевода на модели мелких лабораторных животных, изучена морфологическая структура полученного ацеллюлярного матрикса, проведена количественная оценка остатков ДНК, выполнена рецеллюляризация биологического каркаса мезенхимными мультипотентными стволовыми клетками для оценки цитотоксичности, сохранения клеточной жизнеспособности и метаболической активности, а также сделаны гетеротопические ортотопические...
The aim of the investigation was to study biocompatibility and biomechanical properties recellularized non-woven materials based on collagen-filled polylactide under in vitro vivo conditions assess potential their use for diaphragm defect replacement experiment small laboratory animals.Materials Methods.Non-woven were obtained by electroforming a tight box with subsequent specimen vacuumization.9% aqueous solution prepared solvent mixture: chemically pure chloroform 10% addition ethanol.The...
Objective: to assess the potentials of using indocyanine green fluorescence angiography in evaluating revascularization tissue-engineered construct that was obtained from decellularized biological matrix primate trachea, including mesenchymal stem cells, after heterotopic tracheal allotransplantation. Material and methods. Tracheas were two male hamadryas baboons. After decellularization, 4 cm segments tracheas implanted under lateral part latissimus dorsi healthy primates, one...
Background . Photodynamic Therapy is one of the treatment methods used in modern oncology. Evaluation efficacy vivo photosensitizers on tumor models generally accepted, but photodynamic therapy technique mice not without drawbacks. The purpose study was evaluation with Ehrlich model after subcutaneous and intracutaneous injection cells. Material Methods conducted BAL B/C both sexes. Fotoditazin® Radachlorin® were as photosensitizers. For photoactivation, Alod laser apparatus a wavelength 662...
Тканевая инженерия может стать альтернативным способом лечения и фокусируется на восстановлении, замене регенерации клеток, тканей органов с нарушенными функциями.Одной из задач регенеративной медицины является выбор клеточной линии для рецеллюляризации биологических или синтетических каркасов.Для статической даже целого органа можно использовать мультипотентные мезенхимальные стромальные клетки (ММСК) последующей оценкой их метаболической активности жизнеспособности.Популяция ММСК,...
The aim of the study was to evaluate biocompatibility acellular matrices on model primate lungs, which is closely approximated human lungs in terms anatomic-morphological characteristics, for purpose creating tissue engineering constructions.Materials and Methods.Acellular matrix obtained from detergent-enzymatic decellularization according a modified protocol, where exposition time detergent concentration were increased.The quality scaffold morphologically evaluated after staining with...