A5067758103- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Proteoglycans and glycosaminoglycans research
Cytoskeleton (United States)
2022
Lysine acetylation is an important regulatory post-translational modification (PTM) that occurs sub-stoichiometrically, often representing less than 1% of the target protein. This makes studying endogenous protein extremely challenging. Recent reports suggest several modifications (PTMs), including lysine acetylation, play a major role in regulation programmed cell death-ligand 1 (PD-L1), clinically target. An enrichment step necessary to enable identification acetylated species by either...
Here we present a method that allows detection of acetylated PD-L1 and is applicable to wide range cell lines. The captures >90% species, semi-quantitative simple perform in any lab equipped with tissue culture western blot equipment. involves processing cells lysis buffer has been optimized for efficient immunoprecipitation (IP) an IP enrichment step utilizing acetyl-lysine affinity matrix both total on the same blot. This technique compliments alternative approach antibody as reagent...