A5060696444- Microtubule and mitosis dynamics
- Retinal Diseases and Treatments
- 14-3-3 protein interactions
- Retinal and Optic Conditions
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Protein purification and stability
- Ubiquitin and proteasome pathways
- Plant tissue culture and regeneration
- Protein Kinase Regulation and GTPase Signaling
- Plant Virus Research Studies
- Synthesis and biological activity
- Photosynthetic Processes and Mechanisms
- Sports injuries and prevention
- Transgenic Plants and Applications
- melanin and skin pigmentation
- Gastrointestinal disorders and treatments
- Retinal and Macular Surgery
- Reproductive tract infections research
- Facial Trauma and Fracture Management
- Analytical Chemistry and Chromatography
- Cancer Treatment and Pharmacology
- Balance, Gait, and Falls Prevention
- Microencapsulation and Drying Processes
- Histone Deacetylase Inhibitors Research
University of Maryland, Baltimore
2022-2025
Cytoskeleton (United States)
1998-2022
Einstein Medical Center Philadelphia
2021
University of Miami
2014-2015
Duke University Health System
2014
San Antonio Uniformed Services Health Education Consortium
2007-2008
University of California, Santa Barbara
1993-2001
University of Nottingham
1988-1992
Abstract Background Guidelines recommend anal cancer screening in transgender women (TGW) – particularly those living with HIV (LWH) - using cytology followed by high-resolution anoscopy (HRA) abnormal results. This study examines factors associated completion of HRA TGW cytology. Methods We recruited 80 convenience sampling from 4/2021 9/2023. collected survey data, serum samples, and swabs for HPV testing. were scheduled off-site HRA. Non-completion was defined as being but not completing...
Microtubule dynamic instability underlies many cellular functions, including spindle morphogenesis and chromosome movement. The role of guanosine triphosphate (GTP) hydrolysis in was investigated by introduction four mutations into yeast β-tubulin at amino acids 103 to 109, a site thought participate GTP hydrolysis. Three the increased both assembly-dependent rate average length steady-state microtubules over time, measure instability. fourth mutation did not substantially affect or...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification and biochemical characterization of tubulin from the budding yeast Saccharomyces cerevisiaeAshley Davis, Carleton R. Sage, Leslie Wilson, Kevin W. FarrellCite this: Biochemistry 1993, 32, 34, 8823–8835Publication Date (Print):August 31, 1993Publication History Published online1 May 2002Published inissue 31 August 1993https://pubs.acs.org/doi/10.1021/bi00085a013https://doi.org/10.1021/bi00085a013research-articleACS PublicationsRequest...
<h3>Importance</h3> The follow-up schedule for individuals with eyes treated anti–vascular endothelial growth factor agents proliferative diabetic retinopathy (PDR) requires that patients return frequently monitoring and repeated treatment. likelihood a patient will comply should be consideration in choosing treatment approach. <h3>Objective</h3> To describe completion of scheduled examinations among participants assigned to intravitreous injections ranibizumab PDR multicenter randomized...
Abstract Background Transgender people assigned male at birth (TG-AMAB) have higher rates of anal human papillomavirus (HPV) infection and cancer compared with cisgender populations. In a cohort TG-AMAB in Washington DC, we determined the prevalence epidemiological factors associated high-risk HPV (HR-HPV) cytological abnormalities. Methods an urban academic–community clinic, recruited adults identifying as gender different than their sex birth. Participants provided blood samples, swabs for...
We introduced a threonine-to-glycine point mutation at position 143 in the "tubulin signature motif" 140Gly-Gly-Gly-Thr-Gly-Ser-Gly146 of Saccharomyces cerevisiae β-tubulin. In an electron diffraction model tubulin dimer, this sequence comes close to phosphates guanine nucleotide bound β-tubulin exchangeable E site. Both GTP-binding affinity and microtubule (MT)-dependent GTPase activity isolated from haploid tub2-T143G mutant cells were reduced by least 15-fold, compared control wild-type...
Abstract Microtubule (MT) dynamics vary both spatially and temporally within cells are thought to be important for proper MT cellular function. Because appear closely tied the guanosine triphosphatase (GTPase) activity of β‐tubulin subunits, we examined importance in budding yeast S. cerevisiae by introducing a T107K point mutation into region single gene, TUB2 , known affect assembly‐dependent GTPase MTs vitro. Analysis dynamic behavior video‐enhanced differential interference contrast...
It is presently accepted that the mechanism of action for all anti-tumor tubulin ligands involves perturbation microtubule dynamics during G2/M phase cell division and subsequent entry into apoptosis 1]. In this report, we challenge established dogma by describing a unique caused novel series ligands, halogenated derivatives acetamido benzoyl ethyl ester. We have developed suicide ligand tubulin, which covalently attaches to target shows potent cancericidal activity in tissue culture assays...
In the Solar System, surface of an icy moon is composed irregular ice formations at cryogenic temperatures (<200 K), with oxidized layer and a tenuous atmosphere very low pressure (<10−6 atm). A lander mission, whose aim to collect analyze biological samples from ice, must contain device that collects without refreezing liquid sublimation ice. addition, if are in nature, then precautions be taken ensure do not overheat or mix layer. To achieve these conditions, collector maintain close...
Background: Quadriceps function is an important measure in patients recovering postoperatively. Traditionally, strength measures that require high levels of resistance are contraindicated during the early postoperative phase. Thus it may be helpful to evaluate utilization other tools, such as ultrasound imaging, allow for assessment a position low resistance. Hypothesis: The rectus femoris cross-sectional area (CSA) affected by sex and body composition healthy subjects. Study Design:...
Lysine acetylation is an important regulatory post-translational modification (PTM) that occurs sub-stoichiometrically, often representing less than 1% of the target protein. This makes studying endogenous protein extremely challenging. Recent reports suggest several modifications (PTMs), including lysine acetylation, play a major role in regulation programmed cell death-ligand 1 (PD-L1), clinically target. An enrichment step necessary to enable identification acetylated species by either...