A5070570938- Advanced Electron Microscopy Techniques and Applications
- Advanced X-ray Imaging Techniques
- Electron and X-Ray Spectroscopy Techniques
- Advanced X-ray and CT Imaging
- Digital Holography and Microscopy
- Laser-Plasma Interactions and Diagnostics
- CO2 Reduction Techniques and Catalysts
- Machine Learning in Materials Science
- Catalytic Processes in Materials Science
- Autophagy in Disease and Therapy
- Ammonia Synthesis and Nitrogen Reduction
- Electronic and Structural Properties of Oxides
- Electrocatalysts for Energy Conversion
- Lysosomal Storage Disorders Research
- Systemic Lupus Erythematosus Research
- Photoreceptor and optogenetics research
- Microbial Metabolic Engineering and Bioproduction
- Laser Material Processing Techniques
- Corrosion Behavior and Inhibition
- Advancements in Photolithography Techniques
- TiO2 Photocatalysis and Solar Cells
- Protein Interaction Studies and Fluorescence Analysis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Dyeing and Modifying Textile Fibers
- Atherosclerosis and Cardiovascular Diseases
Helmholtz-Zentrum Berlin für Materialien und Energie
2013-2025
Humboldt-Universität zu Berlin
2023
Fritz Haber Institute of the Max Planck Society
2020-2021
Max-Born-Institute for Nonlinear Optics and Short Pulse Spectroscopy
2013
Abstract Electrocatalysts alter their structure and composition during reaction, which can in turn create new active/selective phases. Identifying these changes is crucial for determining how morphology controls catalytic properties but the mechanisms by operating conditions shape catalyst’s working state are not yet fully understood. In this study, we show using correlated operando microscopy spectroscopy that as well-defined Cu 2 O cubes evolve under electrochemical nitrate reduction...
Abstract The “Seven Pillars” of oxidation catalysis proposed by Robert K. Grasselli represent an early example phenomenological descriptors in the field heterogeneous catalysis. Major advances theoretical description catalytic reactions have been achieved recent years and new catalysts are predicted today using computational methods. To tackle immense complexity high-performance systems where selectivity is a major issue, analysis scientific data artificial intelligence science provides...
Understanding a catalytic reaction requires tools that elucidate the structure of catalyst surface and subsurface, ideally at atomic resolution under conditions. Operando electron microscopy meets this requirement in some cases, but fails others where required conditions cannot be reached or lead to an unwanted influence beam on reactant catalyst. We introduce ILIAS (identical location imaging spectroscopy) combination with quasi situ approach disentangle effect heat gas nanoparticles from...
In electron microscopy, obtaining low-noise images is often difficult, especially when examining biological samples or delicate materials. Therefore, the suppression of noise essential for analysis such noisy images. State-of-the-art image denoising methods are dominated by supervised Convolution neural network (CNN)-based methods. However, CNNs cannot be used if a noise-free ground truth unavailable. To address this problem, we propose method that uses re-occurring patterns in Our proposed...
Fresnel zone plates produced by electron beam lithography and planar etching techniques provide a resolving power of about 10 nm. An alternative plate fabrication approach is based on alternately coating micro-wire with two different materials. With this process, very thin layers high aspect ratios can be deposited. However, depending the method, random positioning errors may introduce strong aberrations. We simulate effect using fluctuations study their influence resolution. find that...
During starvation in the yeast Saccharomyces cerevisiae vacuolar vesicles fuse and lipid droplets (LDs) can become internalized into vacuole an autophagic process named lipophagy. There is a lack of tools to quantitatively assess starvation-induced fusion lipophagy intact cells with high resolution throughput. Here, we combine soft X-ray tomography (SXT) fluorescence microscopy use deep-learning computational approach visualize quantify these processes yeast. We focus on homologs mammalian...
Co-abietate and Cu-abietate complexes were obtained by a low-cost eco-friendly route. The synthesis process used Pinus elliottii resin an aqueous solution of CuSO4/CoSO4 at mild temperature (80 °C) without organic solvents. are functional pigments for commercial architectural paints with antipathogenic activity. characterized Fourier-transform infrared spectroscopy (FTIR), mass spectrometry (MS), thermogravimetry (TG), near-edge X-ray absorption fine structure (NEXAFS), photoelectron (XPS),...
Abstract Memristive devices with valence change mechanism (VCM) show promise for neuromorphic data processing, although emulation of synaptic behavior analog weight updates remains a challenge. Standard filamentary and area‐dependent resistive switching exhibit characteristic differences in the transition from high to low resistance state, which is either abrupt inherently variability or gradual allows quasi‐analog operation. In this study, two modes are clearly correlated microstructure...
We present a versatile and handy method allowing thickness determination of freestanding thin plastic foils by its transmission characteristics in the extreme ultraviolet (EUV) spectrum. The is based on laser induced plasma source, emitting light EUV region, compact double-mirror monochromator operating at fixed wavelength 18.9 nm, CCD camera. measurement delivers values with standard deviation ΔT = 0.005 enabling characterization nm-accuracy given foil density stoichiometric composition....
Micro- and nanoplastics (MNPs) are considered a possible threat to microorganisms in the aquatic environment. Here, we show that total scattering intensity analysis of electron diffraction (ED) data measured by transmission microscopy, which yields pair distribution function (ePDF), is feasible method for characterization identification MNPs down 100 nm. To demonstrate applicability, cryo ball-milled powders most common polymers [i.e., polyethylene , polypropylene, terephthalate, polyamide]...
Get PDF Email Share with Facebook Tweet This Post on reddit LinkedIn Add to CiteULike Mendeley BibSonomy Citation Copy Text J. G. McNally, S. Rehbein, C. Pratsch, Werner, P. Guttmann, and Schneider, "3D PSF Measurement for a Soft X-ray Microscope Comparison Theory," in Imaging Applied Optics 2016, OSA Technical Digest (online) (Optica Publishing Group, 2016), paper CM3D.4. Export BibTex Endnote (RIS) HTML Plain alert Save article
Holography is a powerful method for achieving 3D images of objects. Extending this to short wavelengths potentially offers significantly higher resolution than visible light holography. However, current X-ray holography setups employ nanoscale pinholes form the reference wave. This approach relatively inefficient and limited very small sample size. Here, we propose new setup based on binary diffractive optical element (DOE), which forms at same time object illumination optic located...
Structural analysis of biological membranes is important for understanding cell and sub-cellular organelle function as well their interaction with the surrounding environment. Imaging whole cells in three dimension at high spatial resolution remains a significant challenge, particularly thick cells. Cryo-transmission soft X-ray microscopy (cryo-TXM) has recently gained popularity to image, 3D, intact (∼10μm) details architecture organization near-native state. This paper reports new tool...
<title>Abstract</title> Determining the active phase of an electrocatalyst at work is key to understanding its properties. However, operating morphology electrocatalysts challenging investigate because they can restructure into different motifs under applied potential due changes in their oxidation state. These transformations will further alter catalytic Here, we employ a multi-modal approach centered on electrochemical liquid cell transmission electron microscopy (EC-TEM) study evolution...
7-Nitrobenz-2-oxa-1,3-diazole (NBD) is a widely used fluorescent label for proteins, peptides, and lipids. Its chloride derivative, NBD-Cl, can be highly reactive toward thiol amine groups, forming stable adducts. When labeling the ubiquitous lipid molecule ceramide, NBD-ceramide (NBDCER) aids in visualizing sphingolipid metabolism cells. This study investigates intracellular molecular changes induced by NBD-Cl NBDCER using surface-enhanced Raman scattering (SERS). SERS spectra from...
Abstract Lipophagy is a form of autophagy by which lipid droplets (LDs) become digested to provide nutrients as cellular response starvation. often studied in yeast, Saccharomyces cerevisiae , LDs internalized into the vacuole. There lack tools quantitatively assess lipophagy intact cells with high resolution and throughput. Here, we combine soft X-ray tomography (SXT) fluorescence microscopy use deep learning computational approach visualize quantify yeast. We focus on yeast homologs...
Accumulation of cholesterol in the intima vessel walls is a hallmark developing atherosclerosis.An early event this process digestion oxidized and aggregated low-density lipoprotein (LDL) particles by macrophages (MΦs).In scavengerreceptor mediated process, MΦs attempt to engulf LDL triggering secretion hydrolytic enzymes from lysosomes, which turn hydrolyzes cholesteryl esters (CEs) residing (Fig. 1) [1].The massive generation non-esterified has two consequences; first, become heavily...