We have developed a prototype three-channel microfluidic chip that is capable of generating linear concentration gradient within channel and useful in the study bacterial chemotaxis. The chemical established by diffusing through porous membrane located side wall can be without through-flow where cells reside. As result, movement center caused solely chemotactic response not variations fluid flow. advantages this generator are (i) its ability to produce static gradient, (ii) rapid...

ABSTRACT An ability to monitor bacterial locomotion and collective dynamics is crucial our understanding of a number well-characterized phenotypes including biofilm formation, chemotaxis, virulence. Here, we report the tracking multiple swimming Escherichia coli cells in three spatial dimensions at single-cell resolution using novel three-dimensional (3D) defocused particle (DPT) method. The 3D trajectories were generated for wild-type strain RP437 as well isogenic derivatives that display...

It has been recognized that alternate day calorie restriction (ADCR) or exercise positive effects on cardio-metabolic risk factors. is unclear whether the combined effect of ADCR and (aerobic + resistance training) influences risk. We investigated an 8-week program factors in overweight obese adults. This study randomized 45 but healthy adults (F = 26, M 19; aged about 32 to 40 years) into 4 groups: (n 13), 10), plus 12), control 10) for 8 weeks. Body composition, blood lipids profile,...

In an effort to improve the quality of in vitro produced porcine embryos, we investigated effect brain-derived neurotropic factor (BDNF), a neurotropin family member, on maturation (IVM) oocytes. The expression BDNF and truncated isoforms its receptor, tyrosine kinase B (TrkB), p75 common receptor was detected both follicular cells metaphase-I stage oocytes by RT-PCR. However, mRNA full-length TrkB not found although it cells. pattern confirmed immunohistochemistry. Supplementation with (30...

Metabolic liver disease is associated with obesity, insulin resistance, cardiovascular disease, and metabolic disorders. A Mediterranean diet (MD), known for its anti-inflammatory antioxidant properties, effective in managing various chronic diseases, including diseases. This study aimed to explore the influence of adherence MD on risk steatotic (SLD), dysfunction-associated (MASLD), alcohol-related diseases (ALDs).

Abstract Insulin‐like growth factor (IGF)‐I is a receptor‐mediated autocrine and/or paracrine survival for mammalian embryo development. It known to promote the and development of mouse preimplantation embryos. The present study was designed investigate effects IGF‐I (50 ng/ml), anti‐IGF‐I receptor antibody ng/ml) their combination on porcine Furthermore, mechanism underlying embryotropic evaluated by monitoring incidence apoptosis expression apoptosis‐related genes. In both in vitro...

Background Obesity, especially visceral obesity, is known to be an important correlate for cardiovascular disease and increased mortality. On the other hand, high cardiorespiratory fitness suggested effective contributor reducing this risk. This study was conducted determine combined impact of adiposity, otherwise as fatness, on metabolic syndrome in overweight obese adults. Methods A total 232 individuals were grouped into four subtypes according their level. measured by recovery heart rate...

In this study, we determined the expression of epidermal growth factor (EGF) and its receptor (EGFr) gene, effect exogenous EGF supplementation on preimplantation development porcine somatic cell nuclear transfer (SCNT) embryos. vitro matured gilt oocytes were fertilized with frozen-thawed semen in or reconstructed fetal fibroblasts by SCNT. Experiment 1, total RNA was isolated from oocytes, SCNT, fertilization (IVF) The EGFr mRNA using reverse transcription-polymerase chain reaction...

Menopause is known to compound cardiometabolic disease risk factors, and a deeper understanding of the mechanism this effect needed. Recently, osteoblast-derived protein osteocalcin was found function as regulator glucose fat metabolism. However, there lack studies comparing extent association between metabolism in postmenopausal versus premenopausal women.To examine relationship serum women, we identified well-balanced pairs women matched on propensity score. The interactions levels...

Abstract The present study investigated the effect of lactate/pyruvate supplement in culture medium and chemical activation after electric stimulus on vitro development porcine somatic cell nuclear transfer (SCNT) embryos. In matured gilt oocytes were enucleated, reconstructed with fetal fibroblasts, simultaneously fused/activated using a single pulse 2.0 kV/cm for 30 μsec. Experiment 1, embryos cultured North Carolina State University (NCSU)‐23 supplemented either 5.5 mM glucose (Group A)...

Abstract Background Periodontal diseases are closely connected with insulin resistance (IR) and metabolic syndrome. The Triglyceride Glucose Index (TyG) assesses IR, recently, a few indices combining TyG body composition have emerged higher IR predictive performance than alone. We aimed to examine which TyG‐related parameters most helpful in predicting the risk of periodontal disease. Methods From 2013 2015, data were collected through Korean National Health Nutrition Examination Survey....

Objective. We sought to determine whether patients with systemic lupus erythematosus (SLE) and a presumed primary or reactivated Epstein-Barr virus (EBV) serologic response had evidence of an active EBV infection. Background. Patients SLE often have what appears be response. If these then present fever, fatigue, adenopathy leukopenia, it is not clear symptoms are caused by worsening Establishing the correct diagnosis crucial for management. Methods. examined burden in 13 adolescents All were...

The present study was conducted to examine the comparative efficacy of potassium simplex optimization medium (KSOM) and North Carolina State University (NCSU)-23 supplemented with beta-mercaptoethanol (β-ME) amino acids (AA) on developmental competence porcine in vitro fertilized (IVF) embryos. Four experiments were conducted. KSOM NCSU-23 used culture parthenogenetic (Exp. 1) IVF 2) equally effective supporting embryo development from 1-cell stage blastocysts. 3) 4) acid (AA; 5 μl/ml...

Increasing evidence suggests a close interrelationship between disrupted circadian rhythms and obesity metabolic disturbances. In particular, abdominal obesity, which contributes to the pathogenesis of disease, is associated with clock gene expression. However, little known about relationship expression accurate computed tomography (CT)-based measurements visceral adiposity. Therefore, we examined genes in peripheral blood mononuclear cells (PBMCs) subcutaneous adiposity 75 healthy...

Background. Abdominal obesity and its relative distribution are known to differ in association with metabolic characteristics cardiorespiratory fitness. This study aimed determine an between fitness level abdominal adiposity overweight obese adults. Methods. 228 individuals were classified as either unfit or fit based on their recovery heart rate. Visceral adipose tissue (VAT), subcutaneous (SAT), the visceral-to-subcutaneous ratio (VAT/SAT ratio), cardiometabolic analyzed examine...

The purpose of this study is to identify the association between physical fitness (cardiopulmonary and musculoskeletal) distribution abdominal fat in overweight obese adults.Of total 102 participants, 99 participants completed all measurements. Cardiopulmonary was assessed by maximal oxygen consumption test muscular using 10 repetition max. Visceral adipose tissue (VAT) subcutaneous (SAT) were computed tomography (CT) at level lumbar spine 4-5.Cardiopulmonary inversely associated with both...

Abstract Background Korea is an ‘aged’ society on its way to becoming a ‘super aged’ society. As the population ages, new medical diseases become more prevalent. One such problem sarcopenia. Sarcopenia, by definition, skeletal muscle loss. Furthermore, additional studies have also pointed out smooth In this study, we focused correlation between thyroid hormones and sarcopenia with biochemical viewpoint through creatine kinase. Methods This study was undertaken using Shinchon Severance...

Bitches ovulates immature oocytes at prophase of the first meiotic division and oocyte maturation continues in distal part oviduct for least 48 to 72 h. In most cases, no could be found M II stage earlier than 52 h ovulation, start degenerate after 96 ovulation. So, collection vivo matured canine relies on accurate prediction ovulation time. The present study was designed develop a protocol recovery based once daily measurement serum progesterone (P4) concentration. Blood sample (2 ml)...