A5045623416- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Collaborative Teaching and Inclusion
- Parasitic Infections and Diagnostics
- CRISPR and Genetic Engineering
- Nerve injury and regeneration
- Teacher Education and Assessments
- Hereditary Neurological Disorders
- Education and Technology Integration
- Sperm and Testicular Function
- Education and Critical Thinking Development
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Educational Methods and Media Use
- DNA Repair Mechanisms
- Educational Systems and Policies
- Amoebic Infections and Treatments
- Reflective Practices in Education
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Parental Involvement in Education
- Spine and Intervertebral Disc Pathology
- RNA regulation and disease
- Teacher Education and Leadership Studies
- Sexual Differentiation and Disorders
Cornell University
2006-2024
University of Michigan
2018-2024
University of Sheffield
2024
Pediatrics and Genetics
2024
Universidad de La Laguna
2023
Universidad Fernando Pessoa Canarias
2023
Fundación Canaria de Investigación Sanitaria
2023
Hospital Universitario de Canarias
2023
University of St. Thomas - Minnesota
2021-2022
The University of Texas at Dallas
2015-2019
We have developed a prototype three-channel microfluidic chip that is capable of generating linear concentration gradient within channel and useful in the study bacterial chemotaxis. The chemical established by diffusing through porous membrane located side wall can be without through-flow where cells reside. As result, movement center caused solely chemotactic response not variations fluid flow. advantages this generator are (i) its ability to produce static gradient, (ii) rapid...
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a serious and poorly understood disease. To understand immune dysregulation in ME/CFS, we use single-cell RNA sequencing (scRNA-seq) to examine cells patient control cohorts. Postexertional malaise (PEM), an exacerbation of symptoms following strenuous exercise, characteristic symptom ME/CFS. detect changes coincident with PEM, applied scRNA-seq on the same cohorts exercise. At baseline, ME/CFS patients display classical monocyte...
Abstract Background Corticosteroid receptors include mineralocorticoid (MR) and glucocorticoid (GR) receptors. Teleost fishes have a single MR duplicate GRs that show variable sensitivities to mineralocorticoids glucocorticoids. How these compare functionally tetrapod GR, the evolutionary significance of maintaining two GRs, remains unclear. Results We used up seven steroids (including aldosterone, cortisol 11-deoxycorticosterone [DOC]) ligand specificity binding domains corticosteroid...
3'-untranslated regions (UTRs) specify post-transcriptional fates of mammalian messenger RNAs (mRNAs), yet knowledge the underlying sequences and mechanisms is largely incomplete. Here, we identify two related novel 3' UTR motifs in mammals that transcript degradation. These are interchangeable active only within UTRs, where they often preferentially conserved; furthermore, found hundreds transcripts, many encoding regulatory proteins. We degradation occurs via mRNA deadenylation, mediated...
Alternative splicing is an important and ancient feature of eukaryotic gene structure, the existence which has likely facilitated proteome expansions. Here, we have used intron lariat sequencing to generate a comprehensive profile events in Schizosaccharomyces pombe, amongst simplest organisms that possess mammalian-like splice site degeneracy. We reveal unprecedented level alternative splicing, including selection for over half all annotated introns, hundreds novel exon-skipping events,...
Post-transcriptional gene regulation controls the amount of protein produced from an individual mRNA by altering rates decay and translation. Many sequence elements that direct post-transcriptional have been found; in mammals, most such are located within 3' untranslated regions (3'UTRs). Comparative genomic studies demonstrate mammalian 3'UTRs contain extensive conserved tracts, yet only a small fraction corresponds to recognized elements, implying many additional novel exist. Despite...
ABSTRACT Small RNAs play crucial roles in regulating gene expression during mammalian meiosis. To investigate the function of microRNAs (miRNAs) and small interfering (siRNAs) meiosis males, we generated germ-cell-specific conditional deletions Dgcr8 Dicer mice. Analysis spermatocytes from both knockout lines revealed that there were frequent chromosomal fusions meiosis, always involving one or sex chromosomes. RNA sequencing indicates upregulation Atm miRNA-deficient mice,...
Myalgic encephalomyelitis/chronic fatigue syndrome (ME) is a severe, debilitating disease, with substantial evidence pointing to immune dysregulation as key contributor pathophysiology. To characterize the gene regulatory state underlying T cell in ME, we performed multiomic analysis across subsets by integrating single-cell RNA-seq, and ATAC-seq further analyzed CD8+ subpopulations following symptom provocation. Specific of cells, well certain innate displayed most pronounced ME. We...
The 3′ untranslated regions (3′ UTRs) of mRNAs regulate transcripts by serving as binding sites for regulatory factors, including microRNAs and RNA proteins. Binding such trans -acting factors can control the rates mRNA translation, decay, other aspects biology. To better understand role UTRs in gene regulation, we performed a detailed analysis model mammalian UTR, that Hmga2 , with principal goals identifying complete set elements within single determining extent to which interact affect...
Previous studies have demonstrated that pairing vagus nerve stimulation (VNS) with sounds can enhance the primary auditory cortex (A1) response to paired sound. The neural following VNS-sound in other subcortical and cortical fields has not been documented. We predicted VNS-tone would increase responses tone frequency across pathway. In this study, we VNS presentation of a 9-kHz 300 times day for 20 days. recorded tones from 2,950 sites inferior colliculus (IC), A1, anterior field (AAF),...
The biological impact of microRNAs (miRNAs) is determined by their targets, and robustly identifying direct miRNA targets remains challenging. Existing methods suffer from high false-positive rates are unable to effectively differentiate downstream regulatory changes. Here, we present an experimental computational framework deconvolute post-transcriptional transcriptional changes using a combination RNA-seq PRO-seq. This novel approach allows us systematically profile the miRNA. We refer...
MicroRNAs are essential for spermatogenesis. However, the stage-specific requirements particular miRNAs in male mammalian germ line remain largely uncharacterized. The miR-34 family is, to date, only miRNA proven be necessary production of sperm mammals, though its germline roles poorly understood. Here, we generate and analyze paired small RNA mRNA profiles across different stages development mice, focusing on time points shortly before during meiotic prophase I. We show that addition...
The viability of Cryptosporidium parvum oocysts and Ascaris suum eggs inoculated into aerobic anaerobic digesters was measured. were maintained at 37 degrees C, 47 55 with 10-day detention times. Eggs added to each digester in a single spike or chambers placed the for varying periods. Oocysts inactivated very rapidly all systems as determined by dye permeability assay, > 99% after 10 days 4 2 C. more than digesters. At both within 1 h. digestion around 95% days, but 25% still viable 75%...
ABSTRACT When eukaryotic proteins with multiple disulfide bonds are expressed at high levels in Escherichia coli , the efficiency of thiol oxidation and isomerization is typically not sufficient to yield soluble products native structures. Even when such secreted into oxidizing periplasm or cytoplasm cells carrying mutations major intracellular bond reduction systems (e.g., trxB gor mutants), correct folding can be problematic unless a modulator simultaneously coexpressed. In present study...
SUMMARY ME/CFS is a serious and poorly understood disease. To understand immune dysregulation in ME/CFS, we used single-cell RNA-seq (scRNA-seq) to examine cells cohorts of patients controls. Post-exertional malaise (PEM), an exacerbation symptoms following strenuous exercise, characteristic symptom ME/CFS. Thus, detect changes coincident with PEM, also performed scRNA-seq on the same exercise. At baseline, displayed classical monocytes suggestive inappropriate differentiation migration...
Most online dating users perceive ghosting to be common and expect that there is a chance of being ghosted on platforms (ODPs).The current study extends previous research by gaining qualitative insight into what people believe constitutes behavior, why ghost, how makes them feel.This aimed (a) explore individuals' motivations (b) individuals experiences ghosting, (c) gather the ghosters views definition.A total 12 interviews were conducted.All participants had previously ODPs lived in United...
Abstract The mechanisms that maintain a non-cycling status in postmitotic tissues are not well understood. Many cell cycle genes have promoters and enhancers remain accessible even when cells terminally differentiated state, suggesting their repression must be maintained long term. In contrast, enhancer decommissioning has been observed for rate-limiting the Drosophila wing, tissue where die soon after eclosion, but it unclear if this also occurs other contexts of terminal differentiation....