A5069426894- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Microbial Natural Products and Biosynthesis
- Aquaculture disease management and microbiota
- Marine Sponges and Natural Products
- Coral and Marine Ecosystems Studies
- Bacteriophages and microbial interactions
- Studies on Chitinases and Chitosanases
- Insect symbiosis and bacterial influences
- Salmonella and Campylobacter epidemiology
- Protist diversity and phylogeny
- Marine Bivalve and Aquaculture Studies
- Marine Toxins and Detection Methods
- Marine Biology and Ecology Research
- Enzyme Production and Characterization
- Redox biology and oxidative stress
- Invertebrate Immune Response Mechanisms
- Synthesis and Catalytic Reactions
- Marine Ecology and Invasive Species
- Antimicrobial agents and applications
- Molecular Sensors and Ion Detection
- Marine and coastal plant biology
- Microbial Fuel Cells and Bioremediation
- Plant Gene Expression Analysis
- Methane Hydrates and Related Phenomena
Universidade Federal do Ceará
2017-2022
Universidade Federal da Bahia
2018-2021
Universidade de São Paulo
2010-2020
San Diego State University
2012-2018
Associação Paulista para o Desenvolvimento da Medicina
2018
Universidade de Fortaleza
2017-2018
Universidade Federal do Rio de Janeiro
2009-2014
Faculdade de Tecnologia e Ciências
2014
Universidade Federal de São Carlos
2010-2013
Metagenomics, or sequencing of the genetic material from a complete microbial community, is promising tool to discover novel microbes and viruses. Viral metagenomes typically contain many unknown sequences. Here we describe discovery previously unidentified bacteriophage present in majority published human faecal metagenomes, which refer as crAssphage. Its ~97 kbp genome six times more abundant publicly available than all other known phages together; it comprises up 90% 22% reads virus-like...
Shipworms are marine wood-boring bivalve mollusks (family Teredinidae) that harbor a community of closely related Gammaproteobacteria as intracellular endosymbionts in their gills. These symbionts have been proposed to assist the shipworm host cellulose digestion and shown play role nitrogen fixation. The genome one strain Teredinibacter turnerae , first symbiont be cultivated, was sequenced, revealing potential rich source polyketides nonribosomal peptides. Bioassay-guided fractionation led...
Here we report the complete genome sequence of Teredinibacter turnerae T7901. T. is a marine gamma proteobacterium that occurs as an intracellular endosymbiont in gills wood-boring bivalves family Teredinidae (shipworms). This species sole cultivated member endosymbiotic consortium thought to provide host with enzymes, including cellulases and nitrogenase, critical for digestion wood supplementation host's nitrogen-deficient diet. closely related free-living polysaccharide degrading...
Oxygen minimum zones (OMZs) are oceanographic features that affect ocean productivity and biodiversity, contribute to nitrogen loss greenhouse gas emissions. Here we describe the viral communities associated with Eastern Tropical South Pacific (ETSP) OMZ off Iquique, Chile for first time through abundance estimates metagenomic analysis. The viral-to-microbial ratio (VMR) in ETSP fluctuated oxycline declined anoxic core below one on several occasions. number of genotypes (unique genomes as...
Marine sponges are the oldest Metazoa, very often presenting a complex microbial consortium. Such is case of marine sponge Arenosclera brasiliensis, endemic to Rio de Janeiro State, Brazil. In this investigation we characterized diversity some culturable heterotrophic bacteria living in association with A. brasiliensis and determined their antimicrobial activity. The genera Endozoicomonas (N = 32), Bacillus 26), Shewanella 17), Pseudovibrio 12), Ruegeria 8) were dominant among recovered...
The endemic marine sponge Arenosclera brasiliensis (Porifera, Demospongiae, Haplosclerida) is a known source of secondary metabolites such as arenosclerins A-C. In the present study, we established composition A. microbiome and metabolic pathways associated with this community. We used 454 shotgun pyrosequencing to generate approximately 640,000 high-quality sponge-derived sequences (∼150 Mb). Clustering analysis including sponge, seawater twenty-three other metagenomes derived from animal...
Shipworms are marine bivalve mollusks (Family Teredinidae) that use wood for shelter and food. They harbor a group of closely related, yet phylogenetically distinct, bacterial endosymbionts in bacteriocytes located the gills. This endosymbiotic community is believed to support host's nutrition multiple ways, through production cellulolytic enzymes fixation nitrogen. The genome shipworm endosymbiont Teredinibacter turnerae T7901 was recently sequenced addition potential diazotrophy, also...
Microbiome/host interactions describe characteristics that affect the host's health. Shotgun metagenomics includes sequencing a random subset of microbiome to analyze its taxonomic and metabolic potential. Reconstruction DNA fragments into genomes from metagenomes (called metagenome-assembled genomes) assigns unknown taxa/function facilitates discovery novel organisms. Genome reconstruction incorporates sequence assembly sorting assembled sequences bins, characteristic genome. However,...
Salinivibrios are moderately halophilic bacteria found in salted meats, brines and hypersaline environments. We obtained three novel conspecific Salinivibrio strains closely related to S. costicola, from Socompa Lake, a high altitude Andean lake (approx. 3,570 meters above the sea level).The spp. were extremely resistant arsenic (up 200 mM HAsO42-), NaCl 15%), UV-B radiation (19 KJ/m2, corresponding 240 minutes of exposure) by means phenotypic tests. Our subsequent draft genome ionsequencing...
The Salmonella enterica serovars Enteritidis, Dublin, and Gallinarum are closely related but differ in virulence host range. To identify the genetic elements responsible for these differences to better understand how evolving, we sequenced genomes of Enteritidis strain LK5 Dublin SARB12 compared publicly available P125109, CT 02021853 SD3246 genome sequences. We also sequences from biotype 287/91 Pullorum RKS5078. Using bioinformatic approaches, identified single nucleotide polymorphisms,...
Corals display circadian physiological cycles, changing from autotrophy during the day to heterotrophy night. Such transition offers distinct environments microbial community associated with corals: an oxygen-rich environment daylight hours and oxygen-depleted Most studies of coral reef microbes have been performed on samples taken day, representing a bias in understanding composition function these communities. We hypothesized that physiology alters communities boundary layers. Here we...
Shipworms play critical roles in recycling wood the sea. Symbiotic bacteria supply enzymes that organisms need for nutrition and degradation. Some of these have been grown pure culture capacity to make many secondary metabolites. However, little is known about whether such metabolite pathways are represented symbiont communities within their hosts. In addition, has reported patterns host-symbiont co-occurrence. Here, we collected shipworms from United States, Philippines, Brazil cultivated...
Teredinidae are a family of highly adapted wood-feeding and wood-boring bivalves, commonly known as shipworms, whose evolution is linked to the acquisition cellulolytic gammaproteobacterial symbionts harbored in bacteriocytes within gills. In present work we applied metagenomics characterize microbiomes gills digestive tract Neoteredo reynei, mangrove-adapted shipworm species found over large range Brazilian coast. Comparative grouped gill symbiont community different N. reynei specimens,...
Nutrition in the Teredinidae family of wood-boring mollusks is sustained by cellulolytic/nitrogen fixing symbiotic bacteria Teredinibacter clade. The mangrove Neoteredo reynei popularly used treatment infectious diseases north Brazil. In present work, symbionts N. reynei, which are strictly confined to host's gills, were conclusively identified as turnerae. Symbiont variants obtained vitro able grow using casein sole carbon/nitrogen source and under reduced concentrations NaCl. Furthermore,...
ABSTRACT Microbes associated with marine sponges are considered important producers of bioactive, structurally unique polyketides. The synthesis such secondary metabolites involves type I polyketide synthases (PKSs), which enzymes that reach a maximum complexity degree in bacteria. Haplosclerida sponge Arenosclera brasiliensis hosts complex microbiota and is the source arenosclerins, alkaloids cytotoxic antibacterial activity. In present investigation, we performed high-throughput sequencing...
Metabolomic profiles were explored to understand environmental and taxonomic influences on the metabolism of two congeneric zoanthids, Palythoa caribaeorum P. variabilis, collected across distinct geographical ranges. Integrated mass spectrometry data suggested major influence location chemical divergence when compared species differentiation.
In the present study, we integrated liquid chromatography high-resolution mass spectrometry (LC-HRMS) and high-throughput DNA sequencing for prospecting cytotoxic specialized metabolites from Pseudofusicoccum stromaticum, an endophytic fungus associated to medicinal plant Myracrodruon urundeuva. LC-HRMS profiling allowed identifying putatively eleven compounds in ethyl acetate extract P. stromaticum broth. Additionally, a chemical fractionation guided by cytotoxicity combined with...
ABSTRACT Salmonella enterica subsp. serovar Typhimurium strain YU39 was isolated in 2005 the state of Yucatán, Mexico, from a human systemic infection. The is representative multidrug-resistant emergent sequence type 213 (ST213) genotype. complete genome composed chromosome and seven plasmids.