A5007459435- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Immunotherapy and Immune Responses
- Contact Dermatitis and Allergies
- IL-33, ST2, and ILC Pathways
- Gut microbiota and health
- Diabetes and associated disorders
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Immunodeficiency and Autoimmune Disorders
- Neurological Disorders and Treatments
- Intensive Care Unit Cognitive Disorders
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Resistance in Bacteria
- Respiratory Support and Mechanisms
- Mycobacterium research and diagnosis
- Asthma and respiratory diseases
- Cardiac pacing and defibrillation studies
- DNA Repair Mechanisms
- Cardiomyopathy and Myosin Studies
- Proteins in Food Systems
- Cardiovascular Function and Risk Factors
- Antibiotic Use and Resistance
King's College London
2013-2025
The Francis Crick Institute
2017-2025
Complejo Hospitalario Universitario de Santiago
2018-2024
Complexo Hospitalario Universitario A Coruña
2024
Hospital Ramos Mejía
2024
W. L. Gore & Associates (Germany)
2008-2018
Universidad Complutense de Madrid
2002-2014
The Honourable Society of Lincoln's Inn
2008-2013
Cancer Research UK
2007-2013
John Radcliffe Hospital
2012
The appropriate execution of DNA double-strand break (DSB) repair is critical for genome stability and tumor avoidance. 53BP1 BRCA1 directly influence DSB pathway choice by regulating 5′ end resection, but how this achieved remains uncertain. Here we report that Rif1−/− mice are severely compromised 53BP1-dependent class switch recombination (CSR) fusion dysfunctional telomeres. inappropriate accumulation RIF1 at DSBs in S phase antagonized BRCA1, deletion Rif1 suppresses toxic nonhomologous...
Early events of B cell activation after receptor (BCR) triggering have been well characterized. However, little is known about the steady state BCR on surface. Here, we simultaneously visualize single particles and components membrane skeleton. We show that an ezrin- actin-defined network influenced steady-state diffusion by creating boundaries restrict diffusion. identified intracellular domain Igbeta as important in mediating this restriction Importantly, alteration was sufficient to...
Highly regulated activation of B cells is required for the production specific antibodies necessary to provide protection from pathogen infection. This process initiated by recognition antigen through cell receptor (BCR), leading early intracellular signaling followed late recruitment T help. In this study we demonstrate that BCR uptake CD1d-restricted antigens represents an effective means enhancing invariant natural killer (iNKT)-dependent responses in vivo. mechanism over a wide range...
Highlights•NKT cells promote B cell immunity upon viral infection•NKT are primed by lymph-node-resident macrophages•NKT produce early IL-4 wave at the follicular borders•Early is required for efficient seeding of germinal centersSummaryB constitute an essential line defense from pathogenic infections through generation class-switched antibody-secreting (ASCs) in centers. Although this process known to be regulated helper T (TfH) cells, mechanism which initially seed center reactions remains...
During the activation of humoral immune responses, B cells acquire antigen for subsequent presentation to cognate T cells. Here we show that after mouse accumulate antigen, it is maintained in a polarized distribution extended periods vivo. Using high-throughput imaging flow cytometry, observed this polarization preserved during cell division, promoting asymmetric segregation among progeny. Antigen inheritance correlates with ability progeny activate cells: Daughter receiving larger stores...
Article29 January 2018Open Access Transparent process CD1d-mediated lipid presentation by CD11c+ cells regulates intestinal homeostasis Julia Sáez de Guinoa The Peter Gorer Department of Immunobiology, King's College London, UK Francis Crick Institute, Search for more papers this author Rebeca Jimeno Mauro Gaya Ragon Institute MGH, MIT and Harvard, Cambridge, MA, USA David Kipling Division Cancer Genetics, School Medicine, Cardiff University, Cardiff, María José Garzón...
Abstract Alterations in cellular metabolism underpin macrophage activation, yet little is known regarding how key immunological molecules regulate metabolic programs macrophages. Here we uncover a function for the antigen presenting molecule CD1d control of lipid metabolism. We show that CD1d-deficient macrophages exhibit reprogramming, with downregulation pathways and an increase exogenous import. This rewiring primes enhanced responses to innate signals, as CD1d-KO cells higher signalling...
CBMs (carbohydrate-binding modules) are the most common non-catalytic modules associated with enzymes active in plant cell-wall hydrolysis. They have been frequently identified by amino acid sequence alignments, but only a few experimentally established to carbohydrate-binding activity. A small olive pollen protein, Ole e 10 (10 kDa), has described as major inducer of type I allergy humans. In present study, ability bind several polysaccharides analysed affinity gel electrophoresis, which...
Innate lymphoid cells (ILCs) are a heterogeneous family of immune that play critical role in variety processes including host defence against infection, wound healing and tissue repair. Whether these involved lipid-dependent immunity remains unexplored. Here we show murine ILCs from tissues express the lipid-presenting molecule CD1d, with group 3 (ILC3s) showing highest level expression. Within ILC3 family, natural cytotoxicity triggering receptor (NCR)
Abstract Olive tree (Olea europaea) pollen is a main cause of allergy associated with extensive areas Europe and North America. Ole e 10, small (10.8 kDa) acidic (pI 5.8) protein, has been identified as major allergen from the olive pollen, isolated, characterized. Circular dichroism analysis gave 17% α helix, 33% β sheet, 21% turn for its secondary structure. Based on amino acid sequences tryptic peptides, protein was cloned sequenced. The consists single polypeptide chain 102 aa, signal...
Mucosal-associated invariant T (MAIT) cells are abundant in the lung and contribute to host defense against infections. During bacterial infections, MAIT cell activation has been proposed require receptor (TCR)–mediated recognition of antigens derived from riboflavin synthesis pathway presented by antigen-presenting molecule MR1. can also be activated cytokines an MR1-independent manner, yet contribution MR1-dependent vs. -independent signals functions vivo remains unclear. Here, we use...
Tissue homeostasis is critically dependent on the function of tissue-resident lymphocytes, including lipid-reactive invariant natural killer T (iNKT) cells. Yet, if and how tissue environment shapes antigen specificity iNKT cells remains unknown. By analysing from lymphoid tissues mice humans we demonstrate that their cell receptor (TCR) repertoire highly diverse distinct for various resulting in differential lipid-antigen recognition. Within peripheral recent thymic emigrants exhibit a...
Background: The clinical characteristics in olive pollen allergy are dependent on the antigenic load, allergens profile, and genetic restrictions. Our objective was to determine specific response pattern Ole e 2 10 sensitization at those levels. Methods: We studied 146 patients with seasonal rhinitis and/or asthma positive prick test Olea europaea pollen. IgE against were detected by skin ELISA. HLA‐DRB1 HLA‐DQB1 loci typed polymerase chain reaction sequence‐specific primers method. Results:...
The gut microbiota has a central influence on human health. Microbial dysbiosis is associated with many common immunopathologies such as inflammatory bowel disease, asthma and arthritis. Thus, understanding the mechanisms underlying microbiota-immune system crosstalk of crucial importance. Antibiotic administration, while aiding pathogen clearance, also induces drastic changes in size composition intestinal bacterial communities which can have an impact treatment mice recapitulates long-term...