A5056706115- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Parasitism and Resistance
- DNA Repair Mechanisms
- Plant Stress Responses and Tolerance
- GABA and Rice Research
- Molecular Biology Techniques and Applications
- Plant nutrient uptake and metabolism
- Rice Cultivation and Yield Improvement
- Bacterial Genetics and Biotechnology
Fujian Agriculture and Forestry University
2015-2023
Abstract WRKY transcription factors (TFs) play crucial roles in biotic and abiotic stress responses. However, their thermal response are still largely elusive, especially rice. In this study, we revealed the functions of WRKY10 TF VQ8 protein containing VQ motif rice thermotolerance. Overexpression or loss function increases thermosensitivity, whereas conversely, overexpression enhances accelerates reactive oxygen species (ROS) accumulation chloroplasts apoplasts, it also induces expression...
Arabidopsis MORE AXILLARY GROWTH2 (MAX2) is a key component in the strigolactone (SL) and karrikin (KAR) signaling pathways regulates degradation of SUPPRESSOR OF MAX2 1/SMAX1-like (SMAX1/SMXL) proteins, which are transcriptional co-repressors that regulate plant architecture, as well abiotic biotic stress responses. The max2 mutation reduces resistance against Pseudomonas syringae pv. tomato (Pst). To uncover mechanism MAX2-mediated resistance, we evaluated various SL KAR pathway mutants....
Thermostable DNA polymerase (Taq Pol Ι) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method. The method ammonium sulfate to precipitate the enzyme, and it saved effort money but not time. Moreover, we found that 30–40% activity of Taq I lost at precipitation step, product contained a small amount DNA. We provided novel, simplified low-cost purify Ι after overproduction enzyme Escherichia coli, ethanol instead enzyme. can be directly...