A5101514138- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- COVID-19 Clinical Research Studies
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Hepatitis C virus research
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Antifungal resistance and susceptibility
- Astro and Planetary Science
- Consumer Behavior in Brand Consumption and Identification
- Toxin Mechanisms and Immunotoxins
- Planetary Science and Exploration
- Animal Disease Management and Epidemiology
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- COVID-19 diagnosis using AI
- Liver Disease Diagnosis and Treatment
- Catalytic C–H Functionalization Methods
Guangzhou Eighth People's Hospital
2024-2025
Guangzhou Medical University
2024-2025
University of Toronto
2023-2024
Chongqing City Mental Health Center
2024
Wuhan University of Technology
2024
Jilin University
2016-2024
Second Affiliated Hospital of Chengdu University of Traditional Chinese
2024
South China Agricultural University
2021-2024
Xiangya Hospital Central South University
2024
Central South University
2024
Host anti-viral factors are essential for controlling SARS-CoV-2 infection but remain largely unknown due to the biases of previous large-scale studies toward pro-viral host factors. To fill in this knowledge gap, we perform a genome-wide CRISPR dropout screen and integrate analyses multi-omics data screen, association studies, single-cell RNA-Seq, host-virus proteins or protein/RNA interactome. This study uncovers many that currently underappreciated, including components V-ATPases, ESCRT,...
Abstract The highly contagious SARS-CoV-2 Omicron subvariants severely attenuated the effectiveness of currently licensed vaccines based on ancestral strains administered via intramuscular injection. In this study, we generated a recombinant, replication-incompetent human adenovirus type 5, Ad5-S-Omicron, that expresses BA.1 spike. Intranasal, but not vaccination, elicited spike-specific respiratory mucosal IgA and residential T cell immune responses, in addition to systemic neutralizing...
The continuously evolving Omicron subvariants has diminished the effectiveness of almost all RBD-targeted antibodies in neutralizing these subvariants. development broad-spectrum is desired for addressing both current and future variants. Here, we identified a shark-derived nanobody, 79C11, that can neutralize tested so far, including BA.1 to JN.1 KP.2, exhibits comparable potency against SARS-CoV-1 pangolin coronavirus. Intranasal instillation 79C11 effectively prevent infection subvariant...
Abstract An unusual copper‐catalyzed cyanation of aryl iodides to form nitriles by cyano group transfer from C( sp 3 ) 2 carbon atoms is reported and proceeds give yields up 86%.
The flavonoids were extracted from alfalfa using ethanol assisted with ultrasonic extraction and purified by D101 macroporous resin column chromatography. chemical composition content of elution fractions (EEFs) assessed ultrahigh-performance liquid chromatography hybrid quadrupole time flight mass spectrometry (UHPLC-Q-TOF-MS) aluminum nitrate-sodium nitrite-sodium hydroxide colorimetric method. in vitro antioxidant activity two EEFs was conducted scavenging DPPH free radical, the main...
Porcine circovirus 4 (PCV4) is a newly identified porcine in pigs, belonging to the Circoviridae family Circovirus genus. The virus was detected all age groups and aborted foetuses. However, has not been isolated from field samples date. In this study, PCV4 successfully rescued an infectious clone. replicable PK-15 cells piglets, which can be almost collected of challenge groups. No obvious clinical symptoms were observed both sham- PCV4-inoculated piglets during whole experiment. visible...
Get down with the func(tionalization): An efficient and practical protocol for CH ortho arylation of benzoic acids diaryliodonium salts by using water as an environmentally benign, nontoxic reaction medium is developed. The choice solvent crucial this reaction, which sets stage broad application aqueous conditions in functionalization reactions. As a service to our authors readers, journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized...
Porcine circovirus 2 (PCV2) and pseudorabies virus (PRV) are economically important pathogens in swine. PCV2 PRV coinfection can cause more severe neurological respiratory symptoms higher mortality of piglets. However, the exact mechanism involved its pathogenesis remain unknown. Here, porcine kidney cells (PK-15) were infected with and/or PRV, then activation immune inflammatory pathways was evaluated to clarify influence on responses. We found that promote nuclear factor-κB (NF-κB), c-Jun...
Financial support is a crucial part of China's poverty alleviation effort. Thus, it vital to understand how formal credit impacts income growth in rural households. In 2012, 2015, and 2018, survey was conducted obtain panel dataset 592 households from 6 poverty-stricken counties western China, including Guizhou, Yunnan, Shaanxi provinces. We use the data examine effect on household mechanism that underlies this effect. find can significantly increase households' deprived areas China....
Abstract Background Integrating CRISPR-Cas12a sensors with isothermal signal amplification can be exploited to develop low-cost, disposable, and ultrasensitive assays for the diagnostics of human pathogens. Methods RT-RAA-Cas12a-mediated real-time or end-point fluorescent lateral flow strip (LFS) direct detection norovirus (NOV) genotype GII.4 GII.17 were explored. Results The results showed that our LFS assay could detect NOV by targeting viral protein 1 gene. Our specifically no...
Abstract The upper respiratory tract is the initial site of SARS-CoV-2 infection. Nasal spike-specific secretory immunoglobulin A (sIgA) correlates with protection against Omicron breakthrough We report that intranasal vaccination using human adenovirus serotype 5 (Ad5) vectored spike in people who previously vaccinated ancestral vaccine could induce robust neutralizing sIgA nasal passage. was predominantly present dimeric and multimeric forms accounted for nearly 40% total proteins mucosal...
Starting from December 2019, Wuhan, China, encountered the first outbreak of coronavirus disease 2019 (COVID-19) (1-2).The epidemic was successfully suppressed by strict containment so that number infected people reduced to 0 on April 8, 2020 (3-4).After that, China experienced roughly 3 dozen outbreaks with local transmission caused imported severe acute respiratory syndrome 2 (SARS-CoV-2).These were then contained an effective suppression strategy, and has reached zero again.However,...
Porcine circovirus type 4 (PCV4) is a newly identified porcine (PCV) belonging to the Circovirus genus Circoviridae family. Although several groups have conducted epidemiological investigations on PCV4 and found that also exists widely in pigs, there are few reports origin evolution of PCV4. In this study, genetic relationship between PCV4, mink (MiCV), bat (BtCV), PCV1, PCV2, PCV3 was analyzed, consistency viral proteins three-dimensional (3D) structure epitopes predicted. We genome protein...
Mucosal immunity is crucial for preventing the infection and transmission of respiratory viruses. Nasal antibody inversely correlated with a lower risk However, current reference standard nasal assessment serum-based, mainly consisting monomeric IgG IgA. The applicability serum-derived standards assessing antibodies, mostly dimeric or polymeric secretory IgA (sIgA), remains unvalidated. Herein, we first proved that sera-derived was not applicable antibodies. Using non-homologous as...
Tycho crater is the most prominent of Copernican era, study on whose microwave thermal emission (MTE) can help us better understand internal structures Moon surface. In this paper, spatial and temporal features MTE at area are studied using sounder [Chang'E Lunar Microwave Sounder (CELMS)] data combined with TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> abundance, surface slope roughness, rock abundance lunar regolith. The results...
Porcine epidemic diarrhea (PED) and transmissible gastroenteritis (TGE) caused by porcine virus (PEDV) (TGEV) are two highly contagious intestinal diseases in the swine industry worldwide. Notably, coinfection of TGEV PEDV is common piglets with diarrhea-related diseases. In this study, epithelial cells (IPEC-J2) were single or coinfected and/or TGEV, followed comparison differentially expressed genes (DEGs), especially interferon-stimulated (ISGs), between different groups via...