A5101858256- Immunotherapy and Immune Responses
- Tuberculosis Research and Epidemiology
- Immune Cell Function and Interaction
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune cells in cancer
- Infectious Diseases and Tuberculosis
- Cell Image Analysis Techniques
- Biosensors and Analytical Detection
- T-cell and B-cell Immunology
- RNA modifications and cancer
- Pneumonia and Respiratory Infections
- Central Venous Catheters and Hemodialysis
- Nanoplatforms for cancer theranostics
- Antibiotic Resistance in Bacteria
- Immunodeficiency and Autoimmune Disorders
- Immune responses and vaccinations
- Cancer Genomics and Diagnostics
- Inflammatory Myopathies and Dermatomyositis
South China University of Technology
2023-2025
Roche (United States)
2023-2025
Xinjiang Institute of Materia Medica
2024
Second Xiangya Hospital of Central South University
2023
Central South University
2023
Xuzhou Medical College
2012-2023
Jianghan University
2020-2022
First Affiliated Hospital of Zhengzhou University
2022
Roche (Switzerland)
2022
Tianjin Medical University General Hospital
2021
The proportion of extrapulmonary tuberculosis cases in the United States has increased from 16% cases, 1991, to 20%, 2001. To determine associations between demographic, clinical, and life style characteristics patients with occurrence tuberculosis, a retrospective case-control study was conducted. This included 705 representing 98% culture-proven Arkansas 1 January 1996 through 31 December 2000. A comparison 85 (case patients) 620 pulmonary (control showed women (OR, 1.98; 95% CI,...
The slow growth rate and genetic intractability of tubercle bacilli has hindered progress toward understanding tuberculosis, one the most frequent causes death worldwide. We overcame this roadblock through development near-infrared (NIR) fluorogenic substrates for β-lactamase, an enzyme expressed by bacilli, but not their eukaryotic hosts, to allow real-time imaging pulmonary infections rapid quantification bacteria in living animals a strategy called reporter fluorescence (REF). This...
Smoking is associated with increased susceptibility to tuberculosis and influenza. However, little information available on the mechanisms underlying this susceptibility. Mice were left unexposed or exposed cigarette smoke then infected Mycobacterium by aerosol influenza A intranasal infection. Some mice given a DNA vaccine encoding an immunogenic M. protein. Gamma interferon (IFN-γ) production T cells from lungs spleens was measured. Cigarette exposure inhibited lung T-cell of IFN-γ during...
T cells specific for neoantigens encoded by mutated genes in cancers are increasingly recognized as mediators of tumor destruction after immune checkpoint inhibitor therapy or adoptive cell transfer. Unfortunately, most result from random mutations and patient specific, some contain few to serve potential antigens. We describe a with stage IV acral melanoma who achieved complete response following transfer tumor-infiltrating lymphocytes (TILs). Tumor exome sequencing surprisingly revealed...
Abstract Background Determination of programmed death-ligand 1 (PD-L1) protein expression level in tumor cells and tumor-associated immune is critical for identifying patients eligible immunotherapy. PD-L1 manual scoring algorithms can generally be divided into two categories: cell counting visual estimation. Cell time-consuming not sync with pathology practice, which classically uses a Gestalt approach based on pattern recognition In this study, we introduce the Tumor Area Positivity (TAP)...
Clinical strains of Mycobacterium tuberculosis can be divided into three principal genetic groups based on the single-nucleotide polymorphisms at katG gene codon 463 and gyrA 95. One subgroup group 1, Beijing/W lineage, has been widely studied because its worldwide distribution association with outbreaks. In order to increase our understanding clinical epidemiological relevance grouping M. we investigated 679 isolates tuberculosis, representing 96.3% culture-confirmed cases diagnosed in...
Abstract Current methods for the detection of Mycobacterium tuberculosis (Mtb) are either time consuming or require expensive instruments and thus not suitable point‐of‐care diagnosis. The design, synthesis, evaluation fluorogenic probes with high specificity BlaC, a biomarker expressed by Mtb, described. probe CDG‐3 is based on cephalosporin substitutions at 2 7 positions it demonstrates over 120 000‐fold selectivity BlaC TEM‐1 Bla, most common β‐lactamase. can detect 10 colony‐forming...
Current models propose that reductions in the number of lymphoid-biased hematopoietic stem cells (Ly-HSCs) underlie age-related declines lymphopoiesis. We show Ly-HSCs do not decline with age. Old exhibit changes gene expression and a myeloid-biased genetic profile, but we demonstrate they retain normal lymphoid potential when removed from old vivo environment. Additional studies showing interleukin-1 inhibits Ly-HSC provide support for hypothesis increased production inflammatory cytokines...
Removal of dead cells in the absence concomitant immune stimulation is essential for tissue homeostasis. We recently identified an injury-induced protein misfolding event that orchestrates plasmin-dependent proteolytic degradation necrotic cells. As impaired clearance by innate system predisposes to autoimmunity, we determined whether plasmin could influence endocytosis and cell dendritic – a critical links adaptive systems. find generated on surface enhances their phagocytic removal human...
Ischemia-reperfusion injury (IRI) is one of the leading causes acute kidney (AKI), predisposing patients to chronic disease (CKD) due maladaptive renal repair. Nevertheless, molecular mechanisms and biomarkers that cause repair remain unclear. In this study, we used single-nucleus RNA sequencing data from GEO database (GSE139107) identify markers during transition AKI CKD caused by IRI. Analysis intercellular crosstalk, trajectory machine learning algorithms revealed hub cell clusters genes....
A major limitation to improved outcomes in acute myelogenous leukemia (AML) is relapse resulting from leukemic cells that persist at clinical remission. Regulatory T (Tregs), which are increased AML patients, can contribute immune evasion by residual cells. Tumor necrosis factor (TNF), a pro‐inflammatory cytokine present high levels within induce TNF receptor‐2 (TNFR2) expression on Tregs. We hypothesized since TNFR2 required for Treg stabilization and TNFR2+ Tregs potent suppressors,...
Doxorubicin is the most effective anthracycline chemotherapy drug in treatment of cancer, and it an single agent non-small cell lung cancer (NSCLC). There a lack studies on differentially expressed doxorubicin metabolism-related lncRNAs NSCLC. In this study, we extracted related genes from TCGA database matched them with lncRNAs. lncRNA-based gene signatures (DMLncSig) were gradually screened univariate regression, LASSO multivariate regression analysis, risk score model was constructed....
To identify Mycobacterium tuberculosis virulence factors, we integrated comparative genomics and epidemiologic data analysis to investigate the relationship between certain genomic insertions deletions in phospholipase-C gene D (plcD) with clinical presentation of (TB). Four hundred ninety-six well-characterized M. isolates were studied. Approximately 30% (147) had an interruption plcD gene. Patients infected mutant twice as likely have extrathoracic disease those by a strain without...
ABSTRACT Regions of difference (RDs) have been described in clinical isolates Mycobacterium tuberculosis , but the potential epidemiological and relevance genotypes these RDs remains to be investigated. We screened a population-based sample 648 for deletion five RDs, designated RD105, RD181, RD142, RD150, RD239, using microarray-based hybridization, PCR, DNA sequencing assessed associations between RD deletions characteristics patients chi-square analysis multivariate logistic regression...
Targeted cancer immunotherapy with irradiated, GM-CSF-secreting, allogeneic cell lines has been an effective approach to reduce tumor burden in several patients. It is generally assumed that be effective, these need express immunogenic antigens co-expressed patient cells, and antigen-presenting cells take up such then present them T cells. We have previously reported that, a phase I pilot study (ClinicalTrials.gov NCT00095862), subject stage IV breast experienced substantial regression of...
ABSTRACT The Mycobacterium tuberculosis genome contains four phospholipase C (PLC)-encoding genes, designated plcA , plcB plcC and plcD respectively. Each of the genes contributes to overall PLC activity M. tuberculosis. is hypothesized contribute virulence. Infection strains carrying a truncated gene associated with occurrence extrathoracic However, whether other three plc are also remains be assessed. We investigated insertion- deletion-associated genetic diversity in all among 682...
The slow growth rate of Mycobacterium tuberculosis hinders research progress, since estimating the bacterial numbers present in all experiments normally relies on determination colony forming units agar plates. M. colonies can take as long four to six weeks become visible. Whole animal imaging is an emerging technology that has broad applications areas biological sciences, including monitoring infections. Imaging allows be determined real-time for each infected animal, individually, which...