A5082219130- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Marine Invertebrate Physiology and Ecology
- Mitochondrial Function and Pathology
- Marine Toxins and Detection Methods
- Biotin and Related Studies
- Protein Kinase Regulation and GTPase Signaling
- Photosynthetic Processes and Mechanisms
- Pain Mechanisms and Treatments
- Amphibian and Reptile Biology
- Neuroscience and Neuropharmacology Research
- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Cellular transport and secretion
Jožef Stefan Institute
2019-2024
University of Ljubljana
2020-2023
Jožef Stefan International Postgraduate School
2023
Abstract The β-neurotoxic secreted phospholipases A 2 (sPLA s) block neuro-muscular transmission by poisoning nerve terminals. Damage inflicted such sPLA s (β-ntx) on neuronal mitochondria is characteristic, very similar to that induced structurally homologous endogenous group IIA when its activity elevated, as, for example, in the early phase of Alzheimer’s disease. Using ammodytoxin (Atx), β-ntx from venom nose-horned viper ( Vipera a . ammodytes ), receptor R25 has been detected...
β-Neurotoxins are secreted phospholipase A2 molecules that inhibit transmission in neuromuscular synapses by poisoning the motor neurons. These toxins specifically and rapidly internalise into nerve endings of Ammodytoxin (Atx) is a prototype β-neurotoxin from venom nose-horned viper (Vipera ammodytes ammodytes). Here, we studied relevance enzymatic activity Atx cell internalisation subsequent intracellular movement using electron microscopy (TEM). We prepared recombinant, enzymatically...
People bitten by Alpine vipers are usually treated with antivenom antisera to prevent the noxious consequences caused injected venom. However, this treatment suffers from a number of drawbacks and additional therapies necessary. The venoms Vipera ammodytes aspis neurotoxic cause muscle paralysis inducing neurodegeneration motor axon terminals because they contain presynaptic acting sPLA
Presynaptic- or β-neurotoxicity of secreted phospholipases A
Alzheimer's disease (AD), a progressive form of dementia, is characterized by the increased expression secreted phospholipase A2 group IIA (GIIA) in affected tissue and dysfunction neuronal mitochondria, similar to that induced an orthologous GIIA from snake venom, β-neurotoxic ammodytoxin (Atx), motor neurons. To advance our knowledge about role AD, we studied effect rat on mitochondria compared it with Atx. We produced recombinant (rGIIA) its enzymatically inactive mutant, rGIIA(D49S),...