A5013048798- Lipid metabolism and biosynthesis
- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Cellular transport and secretion
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Sphingolipid Metabolism and Signaling
- Cholesterol and Lipid Metabolism
- Nicotinic Acetylcholine Receptors Study
- Neonatal Respiratory Health Research
- Adipose Tissue and Metabolism
- Fatty Acid Research and Health
- Endoplasmic Reticulum Stress and Disease
- Marine Invertebrate Physiology and Ecology
- Lipid Membrane Structure and Behavior
- Mass Spectrometry Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Traditional and Medicinal Uses of Annonaceae
- Alkaloids: synthesis and pharmacology
- Wnt/β-catenin signaling in development and cancer
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- Biochemical and Structural Characterization
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Jožef Stefan Institute
2014-2024
Jožef Stefan International Postgraduate School
2021
University of Washington
2005
Abstract When the brain is in a pathological state, content of lipid droplets (LDs), storage organelles, increased, particularly glial cells, but rarely neurons. The biology and mechanisms leading to LD accumulation astrocytes, cells with key homeostatic functions, are poorly understood. We imaged fluorescently labeled LDs by microscopy isolated tissue rat astrocytes glia‐like Drosophila determine (sub)cellular localization, mobility, under various stress conditions characteristic for...
Alterations in lipid metabolism are inherent to the metabolic transformations that support tumorigenesis. The relationship between synthesis, storage and use of lipids their importance cancer is poorly understood. human group X secreted phospholipase A2 (hGX sPLA2) releases fatty acids (FAs) from cell membranes lipoproteins, but its involvement regulation cellular FA not known.Here we demonstrate hGX sPLA2 induces droplet (LD) formation invasive breast cells, stimulates proliferation...
Polyunsaturated fatty acids (PUFAs) are structural components of membrane phospholipids and precursors oxygenated lipid mediators with diverse functions, including the control cell growth, inflammation tumourigenesis. However, molecular pathways that availability PUFAs for mediator production not well understood. Here, we investigated crosstalk three in provision production: (i) secreted group X phospholipase A2 (GX sPLA2) (ii) cytosolic IVA PLA2 (cPLA2α), both mobilizing from phospholipids,...
The pulmonary delivery of nanoparticles (NPs) is a promising approach in nanomedicine. For the efficient and safe use inhalable NPs, understanding NP interference with lung surfactant metabolism needed. Lung predominantly phospholipid substance, synthesized alveolar type II cells (ATII), where it packed special organelles, lamellar bodies (LBs). In vitro vivo studies have reported NPs impact on homeostasis, but this phenomenon has not yet been sufficiently examined. We showed that ATII-like...
Ammodytin L (AtnL) is a Ser-49 secretory phospholipase A2 (sPLA2) homologue with myotoxic activity. By analogy to the Lys-49 sPLA2 myotoxins, AtnL has been predicted be enzymatically inactive due absence of conserved Asp-49 that participates in coordination Ca2+ cofactor. substituting and three other residues Ca2+-binding loop AtnL, we obtained first two active mutants Lys-49/Ser-49 homologues. The LW LV, which differed only by presence Trp Val at position 31, respectively, efficiently...
Abstract Background The role of AID/APOBEC proteins in the mammalian immune response against retroviruses and retrotransposons is well established. G to A hypermutations, hallmark their cytidine deaminase activity, are present several retrotransposons. However, non-mammalian retroelement restriction not completely understood. Results Here we provide first evidence anti-retroelement activity a reptilian APOBEC protein. green anole lizard A1 protein displayed potent DNA mutator inhibited ex...
Abstract The β-neurotoxic secreted phospholipases A 2 (sPLA s) block neuro-muscular transmission by poisoning nerve terminals. Damage inflicted such sPLA s (β-ntx) on neuronal mitochondria is characteristic, very similar to that induced structurally homologous endogenous group IIA when its activity elevated, as, for example, in the early phase of Alzheimer’s disease. Using ammodytoxin (Atx), β-ntx from venom nose-horned viper ( Vipera a . ammodytes ), receptor R25 has been detected...
Magneto-mechanical actuation (MMA) using the low-frequency alternating magnetic fields (AMFs) of nanoparticles internalized into cancer cells can be used to irreparably damage these cells. However, in usually agglomerate, thus greatly augmenting delivered force compared single nanoparticles. Here, we demonstrate that MMA also decreases cell viability, with mediated by individual, non-interacting The effect was demonstrated ferrimagnetic (i.e., permanently magnetic) barium-hexaferrite...
The enzymatic activity of ammodytoxins (Atxs), secreted phospholipases A2 (sPLA2s) in snake venom, is essential for expression their presynaptic neurotoxicity, but its exact role the process unknown. We have analyzed detail properties Atxs, mutants, and homologues. apparent rates phospholipid hydrolysis by sPLA2s tested vary up to 4 orders magnitude, all enzymes display a strong preference vesicles containing anionic phospholipids, phosphatidylglycerol or phosphatidylserine (PS), over those...
Significance Oxidative stress, which accompanies sterile inflammation, induces release of extracellular vesicles (stressEVs), activate Toll-like receptor 4 (TLR4), resulting in a pattern gene expression distinct from response triggered by bacterial lipopolysaccharide. The synergy between 15-lipoxygenase and secreted phospholipase A 2 , both are induced is necessary for the formation oxidized lysophospholipids, TLR4 endogenous agonists. Moreover, was sPLA promoted K/BxN serum-induced...
Superparamagnetic iron oxide nanoparticles (SPIONs) have great potential for use in medicine, but they may cause side effects due to oxidative stress. In our study, we investigated the of silica-coated SPIONs on endothelial cells and whether oleic acid (OA) can protect from their harmful effects. We used viability assays, flow cytometry, infrared spectroscopy, fluorescence microscopy, transmission electron microscopy. Our results show that are internalized by cells, where increase amount...
Ammodytoxins (Atxs) are group II phospholipases A2 (PLA2s) with presynaptic toxicity from venom of the snake Vipera ammodytes ammodytes. The molecular basis their neurotoxicity, and that similar PLA2 toxins, is still to be explained. To address this problem, a surface-exposed aromatic residue, Phe24, in N-terminal region most potent Atx, AtxA, was replaced by other (tyrosine, tryptophan), hydrophobic (alanine) polar uncharged (serine, asparagine) residues. mutants were produced bacterial...