A5089836605- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- Immune Response and Inflammation
- CAR-T cell therapy research
- Protease and Inhibitor Mechanisms
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- vaccines and immunoinformatics approaches
- HIV Research and Treatment
- Cell Adhesion Molecules Research
- Hematopoietic Stem Cell Transplantation
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Infant Nutrition and Health
- Cytokine Signaling Pathways and Interactions
- Cancer therapeutics and mechanisms
- NF-κB Signaling Pathways
- Phytochemicals and Antioxidant Activities
- Virus-based gene therapy research
- Wound Healing and Treatments
- Spinal Cord Injury Research
- Cytomegalovirus and herpesvirus research
Blood Transfusion Centre of Slovenia
2016-2025
University of Ljubljana
2006-2025
Ljubljana University Medical Centre
2021
Hudson Institute
2018
John Wiley & Sons (United States)
2018
Celica (Slovenia)
2012-2013
Ljubljana Passenger Transport
2011
Endosomal TLRs play an important role in innate immune response as well autoimmune processes. In the therapy of systemic lupus erythematosus, antimalarial drugs chloroquine, hydroxychloroquine, and quinacrine have been used for a long time. Their suppression endosomal TLR activation has attributed to inhibition acidification, which is prerequisite these receptors. We discovered that chloroquine inhibits only by nucleic acids, whereas it augments TLR8 small synthetic compound, R848. detected...
Abstract Background In immune-mediated diseases of the central nervous system, astrocytes exposed to interferon-γ (IFN-γ) can express major histocompatibility complex (MHC) class II molecules and antigens on their surface. MHC are thought be delivered cell surface by membrane-bound vesicles. However, characteristics dynamics this vesicular traffic unclear, particularly in reactive astrocytes, which overexpress intermediate filament (IF) proteins that may affect trafficking. The aim study was...
Resveratrol is a polyphenol that acts on multiple molecular targets important for cell differentiation and activation. Dendritic cells (DCs) are functionally diverse type represent the most potent antigen-presenting of immune system. In this study, we investigated resveratrol-induced effects DCs during their maturation. Our results show resveratrol induces DC-associated tolerance, particularly when applied DC differentiation. Costimulatory molecules CD40, CD80 CD86 were down-regulated, as...
Cystatin F is a cysteine peptidase inhibitor which, unlike other cystatin family members, targeted to endosomal/lysosomal compartments. It synthesized as an inactive disulphide-linked dimer which then converted active monomer by proteolytic cleavage of 15 N-terminal residues. has been suggested regulate the cytotoxicity natural killer (NK) cells inhibiting major granzyme convertases, cathepsins C and H. To test this hypothesis, we prepared variants analyzed their uptake, subcellular...
Background/Objectives: Mesenchymal stromal cells (MSCs) hold the potential for tumor-targeted gene delivery due to their ex vivo manipulability, low immunogenicity, scalability, and inherent tumor-homing properties. Despite widespread use of viral vectors MSC genetic modification, safety concerns have prompted interest in non-viral alternatives, such as electrotransfer (GET). This study aimed optimize GET parameters MSCs transfection, assess biodistribution after administration, evaluate...
Abstract The maturation status of dendritic cells (DCs) is crucial for effective antigen presentation and initiation the primary immune response. Maturation stimuli cause adhesion immature DCs to extracellular matrix, which accompanied by recruitment CD11b/CD18 [macrophage antigen-1 (Mac-1)] integrin receptor, cytoskeleton reorganization, podosome formation. Cathepsin X, a cysteine protease expressed in other APCs, involved Mac-1 activation. We have shown that during maturation, cathepsin X...
Podosomes, specialized actin‐rich structures in macrophages (Mfs), degrade the extra‐cellular matrix (ECM) and are involved cell migration. On two‐dimensional (2D) surfaces Mfs form spot‐like podosomes at ventral surface that develop into protrusive a three‐dimensional (3D) environment resembling ECM. We have shown tips of these characterized by increased accumulation cysteine cathepsins (Cts) B, X, S, H, L, both human blood monocytic line U‐937. Monocyte‐to‐Mf differentiation induces an...