A5021487619- Microbial Inactivation Methods
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Microfluidic and Bio-sensing Technologies
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Bladder and Urothelial Cancer Treatments
- Metal complexes synthesis and properties
- Ultrasound and Hyperthermia Applications
- Bacillus and Francisella bacterial research
- Glycosylation and Glycoproteins Research
- Ultrasound and Cavitation Phenomena
- Lanthanide and Transition Metal Complexes
- Advanced biosensing and bioanalysis techniques
- bioluminescence and chemiluminescence research
- Ovarian cancer diagnosis and treatment
Institute of Oncology Ljubljana
2015-2025
University of Ljubljana
2021-2025
University of Primorska
2019
Abstract Calcium electroporation (CaEP) is a novel anti-tumour treatment that induces cell death by internalization of large quantities calcium. The effectiveness CaEP has been demonstrated in vitro , vivo and preliminary clinical trials; however, its effects on the vasculature have not previously investigated. Using dorsal window chamber tumour model, we observed affected to same degree normal blood vessels as it disrupted caused eradication necrosis. In all cases, effect was more...
Electrochemotherapy (ECT) exhibits high therapeutic effectiveness in the clinic, achieving up to 80% local tumor control but without a systemic (abscopal) effect. Therefore, we designed combination therapy consisting of ECT via intratumoral application bleomycin, oxaliplatin or cisplatin with peritumoral gene electrotransfer plasmid encoding interleukin-12 (p. t. IL-12 GET). Our hypothesis was that p. GET potentiates effect on and levels potentiation varies depending immune status. tested...
In addition to its direct cytotoxic effects, ablative therapies as electrochemotherapy (ECT) can elicit indirect antitumor effects by triggering immune system responses. Here, we comprehensively analyzed this dual effectiveness of intratumoral ECT with chemotherapeutic drugs bleomycin (BLM), oxaliplatin (OXA), and cisplatin (CDDP). Our aim was determine if act in situ vaccination thereby induce an abscopal effect. By evaluating ECT's potential for vaccination, our goal pave the way future...
High-Grade Serous Ovarian Cancer (HGSOC) is the most common and lethal subtype of ovarian cancer, known for its high aggressiveness extensive genomic alterations.
Background/Objectives: Mesenchymal stromal cells (MSCs) hold the potential for tumor-targeted gene delivery due to their ex vivo manipulability, low immunogenicity, scalability, and inherent tumor-homing properties. Despite widespread use of viral vectors MSC genetic modification, safety concerns have prompted interest in non-viral alternatives, such as electrotransfer (GET). This study aimed optimize GET parameters MSCs transfection, assess biodistribution after administration, evaluate...
AbstractSoft tissue sarcomas pose a challenge for successful treatment with conventional therapeutic methods, therefore newer approaches are considered. In this study, we evaluated the antitumor effect of IL-12 electrogene therapy (EGT) on murine SA-1 fibrosarcoma. The Therapeutic plasmid was injected either intratumorally into subcutaneous nodules or intradermally peritumoral region. We achieved remarkable local and systemic both after single DNA application, significant intratumoral...
Cancer immunotherapy is currently one of the leading approaches in cancer treatment. Gene electrotransfer plasmids encoding interleukin 12 (IL-12) into cells leads to production IL-12, which drives immune cell polarization an antitumoral response. One types that shows great promise targeting tumor under influence IL-12 cytokine milieu macrophages. Therefore, aim this study was evaluate gene antibiotic resistance-free plasmid DNA-encoding murine (mIL-12) mice bearing aggressive B16F10...
Gene electrotransfer (GET) is one of the most efficient non-viral gene therapy approaches for localized transfer multiple genes into tumors in vivo; therefore, it especially promising delivering different cytokines that are toxic if administered systemically. In this study, we used concomitant intratumoral GET two cytokines: tumor necrosis factor alpha (TNFα), a potent cytotoxic cytokine to induce situ vaccination, and interleukin 12 (IL-12), an immunostimulatory boost primed local immune...
Skin is an attractive target for gene electrotransfer. It consists of different cell types that can be transfected, leading to various responses We demonstrate these could controlled by selecting the appropriate electrotransfer parameters. Specifically, application low or high electric pulses, applied multi-electrode array, provided possibility control depth transfection in skin, duration and level expression, as well local systemic distribution transgene. The influence pulse type was first...
Skeletal muscle is an attractive target tissue for delivery of therapeutic genes, since it well vascularized, easily accessible, and has a high capacity protein synthesis. For efficient transfection in skeletal muscle, several protocols have been described, including low voltage electric pulses combination pulses. The aim this study was to determine the influence different parameters electrotransfection on short-term long-term efficiency murine evaluate histological changes treated tissue....
Abstract Background The present study aimed to evaluate the antitumor effectiveness of systemic interleukin (IL)‐12 gene therapy in murine sarcoma models, and its interaction with irradiation tumors metastases. To avoid toxic side‐effects IL‐12 therapy, objective was achieve controlled release after intramuscular electrotransfer. Methods Gene electrotransfer plasmid pORF‐mIL12 performed into tibialis cranialis A/J C57BL/6 mice. Systemic monitored serum mice carrying out two sets different...
Several studies have shown that different control plasmids may cause antitumor action in murine tumor models after gene electrotransfer (GET). Due to the differences GET protocols, plasmid vectors, and experimental models, observed effects were incomparable. Therefore, current study was conducted comparing effectiveness of three using same parameters. We followed cytotoxicity vitro effect vivo pControl, pENTR/U6 scr pVAX1 B16.F10 melanoma cells tumors. Types cell death upregulation selected...
Electrochemotherapy is a tumour ablation modality, based on electroporation of the cell membrane, allowing non-permeant anticancer drugs to enter cell, thus augmenting their cytotoxicity by orders magnitude. In preclinical studies, bleomycin and cisplatin proved be most suitable for clinical use. Intravenous administration electrochemotherapy still not widely accepted in clinics, presumably due its lower antitumor effectiveness, but adjuvant therapy immunomodulatory or vascular-targeting...
High-Grade Serous Ovarian Cancer (HGSOC) is the most common and lethal subtype of ovarian cancer, known for its high aggressiveness extensive genomic alterations. Typically diagnosed at an advanced stage, HGSOC presents formidable challenges in drug therapy. The limited efficacy standard treatments, development chemoresistance, scarcity targeted therapies, significant tumor heterogeneity render this disease incurable with current treatment options, highlighting urgent need novel therapeutic...
The cytomegalovirus (CMV) promoter is one of the most commonly used promoters for expression transgenes in mammalian cells. aim our study was to evaluate role methylation and upregulation CMV by irradiation chemotherapeutic agent cisplatin vivo using non-invasive fluorescence imaging.Murine fibrosarcoma LPB mammary carcinoma TS/A cells were stably transfected with plasmids encoding p21 promoter-driven green fluorescent protein (GFP) gene. Solid tumors induced subcutaneous injection tumor...
Tumor irradiation combined with adjuvant treatments, either vascular targeted or immunomodulatory, is under intense investigation. Gene electrotransfer of therapeutic genes one these approaches. The aim this study was to determine, whether gene plasmid encoding shRNA for silencing endoglin, effectiveness, can radiosensitize melanoma B16F10 tumors.The murine tumors, growing on the back C57Bl/6 mice, were treated by triple and irradiation. antitumor effect evaluated determination tumor growth...