A5052833822- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Cancer Immunotherapy and Biomarkers
- Cervical Cancer and HPV Research
- Cancer Cells and Metastasis
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- CNS Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Global Cancer Incidence and Screening
- Monoclonal and Polyclonal Antibodies Research
- Cutaneous lymphoproliferative disorders research
- Acute Lymphoblastic Leukemia research
- Cancer and Skin Lesions
- Microfluidic and Bio-sensing Technologies
- Viral-associated cancers and disorders
- Ovarian cancer diagnosis and treatment
- Immune cells in cancer
- Lung Cancer Treatments and Mutations
- Sarcoma Diagnosis and Treatment
- Oral health in cancer treatment
- Pituitary Gland Disorders and Treatments
- Blood properties and coagulation
- Thyroid and Parathyroid Surgery
Institute of Oncology Ljubljana
2013-2024
University of Maribor
2022-2024
University of Ljubljana
2020-2024
University Clinical Centre
2004
The COVID-19 pandemic threatens the impact of cervical cancer screening and global elimination goals. As programmes were adjusting to new situation, we evaluated intensity, quality, outcomes in Slovenia first seven months pandemic.Historical observational study on data from a population-based registry. Number cytopathology (screening follow-up), histopathology (diagnostic procedures, invasive procedures number newly diagnosed CIN2+ cases) HPV test results entire Slovenian women population...
In our recent study, we determined the cut-off value of CD20 expression at level 25 000 molecules equivalent soluble fluorochrome (MESF) to be predictor response rituximab containing treatment in patients with B-cell lymphomas. 17.5% patients, who had below value, was significantly worse than rest above value. The proportion low might not benefit from necessarily representative. Therefore aim this study quantify a larger series lymphomas which allow us determine more reliably cut-off....
Electrochemotherapy (ECT) exhibits high therapeutic effectiveness in the clinic, achieving up to 80% local tumor control but without a systemic (abscopal) effect. Therefore, we designed combination therapy consisting of ECT via intratumoral application bleomycin, oxaliplatin or cisplatin with peritumoral gene electrotransfer plasmid encoding interleukin-12 (p. t. IL-12 GET). Our hypothesis was that p. GET potentiates effect on and levels potentiation varies depending immune status. tested...
Monitoring of minimal residual disease (MRD) by flow cytometry (FCM) is a powerful prognostic tool for predicting outcomes in acute lymphoblastic leukemia (ALL). To apply FCM-MRD large, collaborative trials, dedicated laboratory staff must be educated to concordantly high levels expertise and their performance quality should continuously monitored. We sought install unique comprehensive training control (QC) program involving large number reference laboratories within the international...
The purpose of this study was to investigate inflammatory cells in vitreous from patients with proliferative diabetic retinopathy (PDR) using flow cytometric analysis. Twenty-eight PDR requiring vitrectomy because macular traction or tractional retinal detachment were enrolled the (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>28</mml:mn></mml:math>), and 6 hole (MH) formed control group. Samples peripheral venous blood obtained...
Abstract Background To overcome obstacles within the Slovenian organised cervical cancer screening programme, a randomised pilot study of human papillomavirus (HPV) self-sampling among non-attenders was performed, aiming to assess three different approaches. Participants and methods Non-attenders aged 30–64 years from two regions were HPV groups–the opt-in (I1, n = 14.400) opt-out (I2, 9.556), with control group (P, 2.600). Self-collected samples analysed using Hybrid Capture 2 assay....
Different immunohistochemical algorithms for the classification of activated B-cell (ABC) and germinal center (GCB) subtypes diffuse large lymphoma (DLBCL) are applied in different laboratories. In present study, 127 patients with DLCBL were investigated, all treated rituximab cyclophosphamide, hydroxydaunorubicin, oncovin prednisone (CHOP) or CHOP-like regimens between April 2004 December 2010. Multi-tumor tissue microarrays prepared tested according to 4 algorithms: Hans; modified Choi;...
Circulating tumor cell count is an independent prognostic factor in early breast cancer. CTCs can be found the blood of 20% patients prior to neoadjuvant therapy. We aimed assess suitability magnetic-activated separation (MACS) technology for isolation and cytological characterization CTCs. In preclinical part study, lines were spiked into buffy coat samples derived from healthy donors, isolated using MACS. Breast cancer cells with preserved morphology successfully isolated. clinical part,...
Detection of bone marrow involvement (BMI) in diffuse large B-cell lymphoma (DLBCL) typically relies on invasive biopsy (BMB) that faces procedure limitations, while
Abstract Spindle epithelial tumor with thymus‐like differentiation (SETTLE) is a rare of the thyroid gland which occurs predominantly in children, adolescents, and young adults. It usually presents as painless neck or mass only exceptionally diffusely enlarged gland, without metastatic disease at diagnosis. We report on case 12‐yr‐old girl who had for about 1 yr was initially treated thyroiditis. Fine‐needle aspiration biopsy (FNAB) performed 8 mo after first admission. Cytological...
Flow cytometric immunophenotyping (FCI), is widely used in cytology for distinguishing between B-cell lymphoma (BCL) and reactive lymphocytic proliferations (RLP), mainly by identifying monotypic populations. Since this cannot always be determined ratios of surface immunoglobulin light chains (sIg LCs) we wanted to assess if cytoplasmic (cIg) LCs, Bcl-2 polymerase chain reaction (PCR) based clonality analysis can improve accuracy cytological diagnoses BCL.Our study included 98 fine needle...
High-grade serous carcinoma (HGSC) is often associated with ascites at presentation. Our objective was to quantify immune cells (ICs) in prior any treatment given and evaluate their impact on progression-free survival (PFS) overall (OS).Forty-seven patients primary HGSC were included. Flow-cytometric analysis performed detect percentages of CD3+ T (CD4+, CD8+, Tregs, NKT cells), B cells, NK (CD56brightCD16- CD56dimCD16+ subsets), macrophages dendritic (DCs). Furthermore, CD103 expression...
Liquid biopsy is becoming an important source of new biomarkers during the treatment metastatic cancer patients. Using size-based microfluid technology, we isolated circulating tumor cells (CTCs) from breast patients to evaluate their presence and cluster formation, as well megakaryocytes immune-inflammatory blood cells, correlate with clinicopathological data overall survival (OS). In total, 59 (median age 60.4 years) were included in study: 62.7% luminal A/B-like, 20.3% HER2-positive, 17%...
The withdrawal of the iView detection system (iV) forced many cytopathology laboratories, including ours, to substitute immunocytochemical (ICC) staining protocols for routine practice with other systems. Our objective was optimize, validate, and implement ICC using OptiView (OV) EnVision FLEX (EnV) systems, comparing results those obtained iV. Residual cytologic samples known diagnoses were used, testing antibodies ten most common markers in diagnostics (calretinin, Ber-EP4, MOC-31,...
p16/Ki-67 dual immunocytochemical staining (DS) has been proven as a sensitive and specific test for triage of HPV positive women with good reproducibility accuracy. However, implementation the into an organized screening program (OSP) is not easy. The aims this study were to compare performance agreement DS results among three Slovenian cytopathological laboratories involved in national OSP, define cases where can be difficult interpret.Cervical smears obtained from 129 referred colposcopy....
Abstract Background High‐grade serous carcinoma (HGSC) is the most common and aggressive type of ovarian cancer, it often associated with ascites at presentation. The objective this study was to evaluate accuracy cytopathology identify immunophenotypic features HGSC BRCA1/2 mutations from ascites. Methods included 45 patients histologically confirmed primary malignant Immunocytochemistry (ICC) staining for PAX8, WT1, P53, P16, Ki‐67 performed on cytospins cytoblocks prepared Next‐generation...
Objective: The phenomenon of cancer‐to‐cancer metastasis cutaneous squamous cell carcinoma (SCC) and chronic lymphocytic leukaemia (CLL)/small lymphoma (SLL) is a rare event only occasionally documented in the medical literature. Methods: Two patients with SCC skin that were previously treated for CLL are presented. Both had palpable lymph nodes neck fine needle aspiration cytology (FNAC) was performed to evaluate pathological process. In addition, literature on metastatic CLL/SLL special...
Chronic lymphocytic leukemia (CLL) typically pursues a prolonged course. Its transformation into more aggressive lymphoma occurs in 2–8% of all patients. Most commonly, diffuse large B-cell develops. Transformation classical Hodgkin’s (cHL) &#x3c;1%. Plasmablastic has been only rarely reported. Cases synchronous divergent CLL composite are exceedingly rare. We describe the unique occurrence long-standing clonally related cHL as well plasmablastic (PBL) an 85-year-old female patient....
Immunotherapy has been successful in treating advanced melanoma, but a large proportion of patients do not respond to the treatment with immune checkpoint inhibitors (ICIs). Preclinical and small cohort studies suggest gastrointestinal microbiome composition exosomal mRNA expression PD-L1 IFNγ from primary tumor, stool body fluids as potential biomarkers for response.Patients treated first line metastatic melanoma are recruted this prospective study. Stool samples submitted before start...
Tumor spheroids in the ascites of high-grade serous carcinoma (HGSC) are poorly described. Our objective was to describe their morphological features, cellular composition, PD-1 and PD-L1 expression, survival correlation these parameters. The density size were assessed Giemsa-stained smears; cell composition spheroids, including tumor cells, immune capillaries, myofibroblasts, as well expression on cells immunocytochemically stained block sections. Forty-seven patients with primary HGSC...
Abstract Background p16/Ki-67 dual immunocytochemical staining (DS) is considered easy to interpret if evaluators are properly trained, however, there no consensus on what constitutes proper training. In the present study we evaluated a protocol for teaching DS evaluation students inexperienced in cervical cytology. Methods Initial training 40 conventional smears was provided by senior cytotechnologist experienced such evaluation. Afterwards, two 118 cases. Additional consisted mainly of...
Flow cytometry is helpful in differentiating between B-cell lymphoma (BCL) and reactive lymphocytic proliferation (RLP) FNA biopsies. However; the presence of inconclusive surface immunoglobulin light chains (sIg LC) poses a problem. We investigated usefulness additional tests; namely Bcl-2 expression cytoplasmic Ig LC (cIg LC), mainly on samples with sIg LC. Both tests were performed 232 from lymph nodes. alone was determined qualitatively quantitatively 315 samples. The quantitative test...
The Ki-67 proliferative index (PI) is part of the diagnosis nodal B-cell lymphoma (nBCL), but its determination in cytological samples not standardized. We aimed to establish an approach for accurate PI slides differentiate between indolent and aggressive nBCLs.