A5002973767- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Chronic Lymphocytic Leukemia Research
- IL-33, ST2, and ILC Pathways
- Childhood Cancer Survivors' Quality of Life
- Lymphoma Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Lung Cancer Research Studies
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- Hemoglobinopathies and Related Disorders
- Epigenetics and DNA Methylation
- COVID-19 and healthcare impacts
- Hematological disorders and diagnostics
- Spondyloarthritis Studies and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Neuroblastoma Research and Treatments
- Genomic variations and chromosomal abnormalities
University of Debrecen
2016-2025
Risk-adapted treatment protocols conferred remarkable improvement in the survival rates of pediatric acute lymphoblastic leukemia/lymphoma (ALL/LBL). Nevertheless, clinical management is still challenging certain molecular subgroups and presence alterations associated with an increased rate relapse. In this study, disease-relevant genomic transcriptomic profiles were established a prospective, multicenter, real-world cohort involving 192 children diagnosed ALL/LBL. Gene fusions detected...
Monitoring of minimal residual disease (MRD) by flow cytometry (FCM) is a powerful prognostic tool for predicting outcomes in acute lymphoblastic leukemia (ALL). To apply FCM-MRD large, collaborative trials, dedicated laboratory staff must be educated to concordantly high levels expertise and their performance quality should continuously monitored. We sought install unique comprehensive training control (QC) program involving large number reference laboratories within the international...
Patients with hematological malignancies (HMs) are at a higher risk of developing severe form and protracted course COVID-19 disease. We investigated whether the combination viral replication inhibition remdesivir administration anti-SARS-CoV-2 immunoglobulins convalescent plasma (CP) therapy might be sufficient to treat B-cell-depleted patients COVID-19. enrolled 20 consecutive various HMs profound B-cell lymphopenia pneumonia between December 2020 May 2021. All demonstrated undetectable...
Background Given the physiological hemostasis changes during pregnancy, limited data exists on COVID-19-induced inflammatory response and alterations in pregnant women. Objectives To test a comprehensive set of cytokines pregnancies with/without COVID-19 correlate results with maternal perinatal outcomes. Patients/methods In this observational case-control study, 100 women acute at 24-40 gestational weeks (COVID-19+ group), healthy, age- week-matched, SARS-CoV-2 negative (32 proven recovery...
Abstract Unconventional T cells show distinct and unique features during antigen recognition as well other immune responses. Their decrease in frequency is associated with various autoimmune disorders, allergy, inflammation, cancer. The landscape of the unconventional altogether (iNKT, γδ T, MAIT) largely unestablished leading to challenges affecting diagnosis research this field. In study, we have established age group–wise iNKT, MAIT on a total 203 healthy adult samples Caucasian...
This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2+/CD161− in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate adaptive immunity, were analyzed alongside peripheral blood samples from 14 dermatitis patients 10 healthy controls. Flow cytometry machine learning algorithms employed a comprehensive analysis. The results indicate significant decrease CD69 subsets dermatitis, coupled with elevated CD38 polyfunctional...
Abstract Genotyped human B-lymphoblastoid cell lines (LCLs) are widely used models in mapping quantitative trait loci for chromatin features, gene expression, and drug response. The extent of genotype-independent functional genomic variability the LCL model, although largely overlooked, may inform association study design. In this study, we use flow cytometry, immunoprecipitation sequencing mRNA to surface marker patterns, quantify genome-wide changes (H3K27ac) transcriptome variability,...
Abstract This study tested the hypothesis of gender bias in frequency unconventional T cells. Unconventional cells exist as minor subsets peripheral blood. Despite their low number, they play a crucial role various immune-mediated diseases such inflammation, autoimmunity, allergy, and cancer. Gender-based these altogether on large number healthy individuals are unestablished creating hurdles to manifest association with pathologic conditions. In this study, we used multicolor flow cytometric...
Summary Blood coagulation factor XIII (FXIII) is a protransglutaminase circulating asa tetramer formed by two types of subunits (A2B2). The intracellular dimeric form FXIII (A2) present in platelets, megakaryocytes, monocytes and macrophages has been detected mono-and megakaryocytic leukemias. aim our study was to investigate FXIII-A expression newly diagnosed B cell acute lymphoblastic leukemia (ALL) samples. We examined 47 de novo ALL cases origin triple color labeling with flow cytometry....
Abstract Leukemic cells often express markers, which are not characteristic of their particular cell lineage. In this study, we identified the “A” subunit coagulation factor XIII (FXIII‐A) in leukemic promyelocytes de novo AML M3 cases. The cytoplasmic presence XIII‐A has previously been shown only platelets/megakaryocytes and monocytes/macrophages. Furthermore, more recently described FXIII‐A lymphoblasts. We studied 14 patients with rare type acute leukemia a period 4 years investigated...
Disseminating cells of a primary solid tumor may represent the origin metastases and relapses. We aimed at comparing diagnostic efficacy multicolor flow cytometry (MFC) morphology/immunohistochemistry (IHC) in detection disseminated bone marrow (BM) body fluids patients with tumors, pediatric neuroblastoma cases. investigated 72 samples retrospecively from 50 by MFC. Morphology/IHC data were available 48 In first cohort, 36 derived 34 various forms suspected proven tumors second 16 children...
Acute promyelocytic leukemia (APL) is generally characterized by t(15;17)(q24;q21). In some cases, the classic translocation cannot be identified conventional methods, since PML-RARA fusion protein results from complex, variant, or cryptic translocation. The diagnostic algorithm of APL starts with screening such as flow cytometry (FC), followed fluorescence in situ hybridization polymerase chain reaction to confirm diagnosis. Our aim was develop a novel protocol for analyzing samples based...
Abstract As the association of human leukocyte antigen B27 (HLA‐B27) with spondylarthropathies is widely known, HLA‐B27 expression frequently identified using flow cytometric or other techniques. Because possibility cross‐reaction off target antigens, such as HLA‐B7, each technique applies a “gray zone” reserved for equivocal findings. Our aim was to use machine learning (ML) methods classify data positive negative. Equivocal samples ( n = 99) were selected from submitted our institution...
Background SARS-CoV-2 infection during pregnancy increases the risk of severe obstetrical complications. Detailed evaluation COVID-19-associated coagulopathy in a with stillbirth hasn’t been described so far. Besides knowledge gaps pathomechanism leading to COVID-19 pregnancies, currently, no prognostic biomarker is available identify pregnant patients who are at imminent maternal and fetal complications, requiring immediate medical attention. Case Here we report case 28-year-old infected...
Mucosal-associated invariant T (MAIT) cells, a subset of Vα7.2+ are crucial link between innate and adaptive immunity, responding to various stimuli through TCR-dependent independent pathways. We investigated the responses MAIT cells Vα7.2+/CD161- different evaluated effects Cyclosporin A (CsA) Vitamin D3 (VitD). Peripheral blood mononuclear (PBMCs) from healthy donors were stimulated with agents (PMA/Ionomycin, 5-OP-RU, 5-OP-RU/IL-12/IL-33) or without CsA VitD. Flow cytometric analysis...
Background To date no age-related reference ranges are available for serum thymidine kinase (TK1) activity. Being a proliferation marker, it may be used as prognostic marker in malignant diseases, including chronic lymphocytic leukemia (CLL). Our aim was to establish age-specific TK1 and examine its utility screening CLL, disease of the elderly. Methods Serum activity measured by competitive chemiluminescent immunoassay 369 healthy adults 115 de novo CLL patients. Results We observed...
A growing body of evidence supports the usefulness dysplastic signs detected by flow cytometry in diagnosis myelodysplastic syndromes (MDS). Our aim was to assess impact pre-analytical variables (delayed sample handling, type anticoagulant, and different clones antibody) interpretation cytometric results.Bone marrow samples were labelled analysed immediately after aspiration on two consecutive days. The effect anticoagulant evaluated 16 bone samples. Thirty-seven immunophenotypic recorded...
Background: Leukemic B-cell precursor (BCP) lymphoblasts were identified as a novel expression site for coagulation factor XIII subunit A (FXIII-A). Flow cytometry (FC) revealed three distinct patterns, i.e., FXIII-A negative, dim, and bright subgroups. The negative subgroup was significantly associated with the "B-other" genetic category had an unfavorable disease outcome. Methods: RNA extracted from bone marrow of 42 pediatric patients BCP-acute lymphoblastic leukemia (ALL). determined by...
Anemia is a severe global public health issue. Testing practices for anemia suggest overuse of screening laboratory tests and misinterpretation studies even in "easy-to-diagnose" underlying causes, leading to late diagnoses missed treatment opportunities. We aimed develop complete efficient algorithm clinical pathologists medicine physicians the differential diagnosis anemia.
Smudge cells (Gumprecht shadows) are chronic lymphocytic leukaemic ruptured during peripheral blood smear preparation. It has been demonstrated to be linked reduced expression of the cytoskeletal protein vimentin and its inverse correlation with clinical outcome disease.Investigation percentage smudge cells, CD38-, ZAP-70-positive time treatment in patients leukaemia.Authors investigated CD38- 50 leukaemia their treatment.21 required follow-up period. Their median cell was 9.9%, while it...
Abstract A new flow cytometric (FC) BCR-ABL immunobead assay has been developed recently. Here we present the laboratory evaluation of commercially available kit. Mononuclear cells were isolated, lysed and processed according to instructions manufacturer. Anti-BCR antibodies adsorbed capture beads bind fusion proteins cells, a phycoerythrin (PE)-conjugated anti-ABL antibody is detector reagent mean fluorescence intensity (MFI) signals recorded by cytometry. Detection t(9;22)(q34;q11)...