Abstract Lipids are important components in human nutrition; however, their increased intake contributes to the development of obesity and can lead multiple long‐term complications. Pancreatic lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) is a key enzyme for absorption dietary triglycerides. Interference with fat hydrolysis results reduced utilization ingested lipids, therefore inhibition lipases decreases absorption. Extracts from 106 species medicinal plants, vegetables fruits were...

Reptiles (sauropsids) represent the sister group to mammals, and basal members of Reptilia may provide a good model for condition common ancestor both groups. Sex-determining mechanisms (SDM) organizations sex chromosomes among genotypically sex-determining (GSD) species vary widely across reptiles. Birds snakes, example, are entirely GSD whereas other reptiles, like all crocodilians, exhibit temperature-dependent determination (TSD). Here we explore evolution SDM within using family-level...

Dried buckwheat herb is used in medicinal products whereas fresh green plant parts, especially sprouts, are consumed as a vegetable. The contains fagopyrins, which cause sensitivity to light after ingestion. aim of this study was investigate the impact different growing conditions and development phase on content fagopyrin phenolic compounds sprouts. Total flavonoid total phenol contents, antioxidant activity were determined spectrophotometrically. Fagopyrin flavonoids located almost...

Discovery and development of new antibiotics is still very important because the constant appearance drug-resistant pathogenic bacteria. The broth microdilution test was applied for screening antibacterial activity in extracts higher endophytic fungi. Among tested extracts, three possessed significant activity: mushrooms Amanita virosa. (Fr.) Bertill. (Amanitaceae) Cortinarius praestans. Cordier (Cortinariaceae) against Pseudomonas aeruginosa. Staphylococcus aureus., respectively, extract...

Abstract: Tartary buckwheat ( Fagopyrum tataricum) seeds have a strong aroma that characteristically differs from the of common esculentum). Its phytochemical background has only been recently investigated. The aim this study was to identify and quantify individual compounds responsible for tartary aroma. Volatiles different samples (whole seed, flour, husks) were extracted with simultaneous extraction distillation by Likens–Nickerson apparatus analyzed GC‐MS. A total 48 quantified their...

Pseudoautosomal regions (PARs) shared by avian Z and W sex chromosomes are typically small homologous within which recombination still occurs hypothesized to share the properties of autosomes. We capitalized on unusual structure emus, Dromaius novaehollandiae, consist almost entirely PAR both chromosomes, test this hypothesis. compared recombination, linkage disequilibrium (LD), GC content, nucleotide diversity between pseudoautosomal autosomal loci derived from 11 emu bacterial artificial...

ABSTRACT Buckwheat is a pseudocereal with strong characteristic aroma. Compounds responsible for the aroma of buckwheat groats were recently identified, but distribution aromatic compounds between different fractions kernel (flour, bran, and husk) not yet known. In this study, composition in seed was investigated compared to produced from same batch seeds. Volatiles each sample extracted simultaneous distillation/extraction Likens‐Nickerson apparatus. Extracts analyzed by gas chromatography...

Many plant extracts and essential oils show antibacterial antifungal activities, with potential to replace the use of synthetic fungicides. We used invasive alien plants goldenrod (Solidago spp.) Japanese knotweed (Fallopia japonica) as source materials determine their activities against seed-associated fungi from wheat grain (Alternaria alternata, Alternaria infectoria, Aspergillus flavus, Epicoccum nigrum Fusarium poae). Aqueous organic (ethanol, methanol acetone) were prepared leaves...

Genomic resources for studies of nonavian reptiles have recently improved and will reach a new level access once the genomes painted turtle (Chrysemys picta) green anole (Anolis carolinensis) been published. Eleven speakers gathered symposium on reptilian genomics evolutionary genetics at 2008 meeting Society Integrative Comparative Biology in San Antonio, Texas. Presentations described results genetic concerning molecular evolution, chromosomal genomic architecture, population dynamics,...

Cathepsin H indirectly regulates morphogenetic protein-4 (BMP-4) in various human cell linesBackground. is a cysteine protease considered to play major role tumor progression, however, its precise function tumorigenesis unclear. was recently proposed be involved processing of bone protein 4 mice. In order clarify whether cathepsin also BMP-4 humans, impact on expression, and degradation investigated prostate cancer (PC-3), osteosarcoma (HOS) pro-monocytic (U937) lines.Materials methods....

Abstract Pumpkin seed oil has a distinct taste and characteristic green‐red colour. A green layer (chlorenchyma) can be observed in the cross section of on inner side testa. This is considered to source protochlorophyll pigment that gives colour pumpkin oil. However, histolocalisation by selective method was not yet studied. In this study, we localised small lipid droplets cotyledon cells Rhodamine 6G lipophilic stain. Furthermore, chlorenchyma microscopic fluorimetry. Emission maximum 700...

An experiment examining the influence of multiple fertilizer applications throughout two-year wild blueberry production cycle was examined at two commercial fields in Nova Scotia and Prince Edward Island during 2000 2001. A randomized complete block experimental design with nine treatments, five replicates, a plot size 6 x 8 m used. The treatments used consisted (1) no application, (2) an initial application 200 kg.ha -1 14-14-14 (N:P 2 O 5 :K O) first year (IFA), (3) IFA + 10 kg N.ha prior...

Abstract The Major Histocompatibility Complex (MHC) is a large gene family that found in most vertebrates and has an important influence on body odour preference mate selection animals. In this research we found, human leukocyte antigen (HLA) phenotype strongly connected with the strength pleasantness of perceived selected chemical compounds sweat. Among different classes tested, esters fatty acids such as methyl undecanoate, decanoate, nonanoate, octanoate hexanoate show strongest...

Abstract Due to severe toxicity of pyrrolizidine alkaloids, their quantification in medicinal products is very important. The idea this research was use retrorsine as a surrogate reference compound instead lycopsamine or isolated from comfrey. A method for the analysis extracts comfrey roots developed and validated, employing thin layer chromatography, derivatisation with Dann-Mattocks reagent followed by densitometric analysis. new showed linearity within 0.70 7.0 μg per application 10 μL...

An improved method for the isolation and purification of pyrrolizidine alkaloids from comfrey (Symphytum officinale L.) roots was developed, introducing very fast, selective ion residue-free reduction N-oxides followed by ion-exchange chromatography giving a non-aqueous solution alkaloids, which solvents can be easily removed. With this procedure use large volumes organic solvents, slow input additional impurities avoided. Lycopsamine, proved to major alkaloid, additionally purified...

Screening new organisms for antibacterial activity and searching drugs is important due to the constant generation of antibiotic-resistant strains pathogenic bacteria. An E. coli broth microdilution test was used evaluate results Vibrio fischeri bioluminescence in five most antibacterially active species wood-colonizing fungi. Serpula lacrymans found be a potential source thermostable antibiotic(s) confirmed useful method screening activity.

A locus that we name SubA was discovered during large-scale sequencing and characterization of a bacterial artificial chromosome library from an emu, Dromaius novaehollandiae. This yields significantly negative Tajima's D in emus is conserved across chicken, mouse, human. Expression orthologs has been reported human ovaries mouse testes, but remains unknown emus. The physically mapped onto pair microchromosomes by fluorescent situ hybridization also chicken as previously reported. By...

Dried buckwheat herb (Fagopyrum esculentum Moench) is used in medicinal products and the fresh green plant parts, especially sprouts, are consumed as a vegetable [1,2]. The contains fagopyrins, which cause sensitivity to light after ingestion of large amounts parts [3]. aim this study was investigate impact different growing conditions development phase on content fagopyrin phenolic compounds sprouts. Total flavonoid total phenol contents, antioxidant activity were determined...