A5070690795- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Immunotherapy and Immune Responses
- Celiac Disease Research and Management
- Renal Diseases and Glomerulopathies
- Hormonal Regulation and Hypertension
- Systemic Lupus Erythematosus Research
- Renal and Vascular Pathologies
- Apelin-related biomedical research
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Birth, Development, and Health
- Immunodeficiency and Autoimmune Disorders
- Immune responses and vaccinations
- Digestive system and related health
- vaccines and immunoinformatics approaches
- Advanced biosensing and bioanalysis techniques
- Sexual Differentiation and Disorders
- Prion Diseases and Protein Misfolding
- Hemostasis and retained surgical items
- Polyomavirus and related diseases
Blood Transfusion Centre of Slovenia
2007-2024
Ljubljana University Medical Centre
2008
Shared susceptibility alleles in the HLA region contribute to co-existence of type 1 diabetes (T1D) and celiac disease (CD). The aim our study was identify genotype variations that influence co-occurrence T1D CD (T1D + CD) order their onset. Totally 244 patients, 67 with T1D, 68 69 CD, (split into "T1D first" "CD first"), were analyzed. Control group consisted 130 healthy unrelated individuals. Two-tailed Fisher's exact test used for statistical analysis. genetic background Slovenian...
In the past, identification of HLA alleles was limited to sequencing region gene coding for peptide binding groove, resulting in a lack sequence information database, challenging allele assignment software programs. We investigated full-length sequences 19 class I and 7 II alleles, we extended another 47 with 5' 3' UTR regions that were all not yet available IPD-IMGT/HLA database. resolved 8638 unknown nucleotides 2139 II. Furthermore, 26 more than 90 kb added non-coding sequences, whereas...
Summary HLA‐NET (a European COST Action) aims at networking researchers working in bone marrow transplantation, epidemiology and population genetics to improve the molecular characterization of HLA genetic diversity human populations, with an expected strong impact on both public health fundamental research. Such improvements involve finding consensual strategies characterize populations samples report typings ambiguities; proposing user‐friendly access databases computer tools defining...
In this prospective, randomized, open-label, single-center study, we compared the efficacy and safety of two anti-interleukin-2 receptor monoclonal antibodies combined with triple immunosuppression.The adult recipients at least one human leukocyte antigen-mismatched deceased donor renal graft on cyclosporine microemulsion, mycophenolate mofetil, methylprednisolone were randomized to induction basiliximab or daclizumab, given in standard doses. An intent-to-treat analysis 1-year data assessed...
Celiac disease (CD) is more common in individuals with insulin dependent diabetes mellitus (T1D) than the general population. HLA class II molecules DQ8 (DQB1*0302-DQA1*0301) and DQ2 (DQB1*0201-DQA1*0501) have been identified as key genetic risk factors both diseases. While conveys a higher for T1D, frequent CD. Less known about contribution of I. The gut immune system has implicated pathogenesis MICA, which mainly expressed gastrointestinal epithelium recognized by γδT lymphocytes natural...
Summary We present here the results of Analysis HLA Population Data ( AHPD ) project 16th International and Immunogenetics Workshop (16 IHIW held in Liverpool May–June 2012. Thanks to collaboration 25 laboratories from 18 different countries, genotypic data for 59 new population samples (either well‐defined populations or donor registry samples) were gathered 55 analysed statistically following ‐ NET recommendations. The included, among others, large sets north‐east Europe West Asia, as well...
Abstract The prevalence of celiac disease ( CD ) in patients with type 1 diabetes T1D has been reported to be 5–7 times higher than the general population. Risk factors for co‐occurrence both diseases have not entirely established. aim our study was analyze possible impact human leukocyte antigen HLA class I and killer cell immunoglobulin‐like receptors KIRs on . We analyzed 67 , 68 69 + 130 controls. Statistical analysis based two tailed Fisher exact test corrections multiple testing. After...
Genetic polymorphisms in genes coding for inflammasome components nucleotide-binding oligomerization domain leucine rich repeat and pyrin domain-containing protein 3 (NLRP3) caspase recruitment 8 (CARD8) have been associated with autoinflammatory autoimmune diseases. On the other hand several studies suggested that NLRP3 contributes to maintenance of gastrointestinal immune homeostasis activation is regulated by tyrosine phosphatase non-receptor 22 (PTPN22). PTPN22 polymorphism was...
OBJECTIVE: To study the incidence of 21-hydroxylase deficiency in Slovenian hyperandrogenic women, at gene level. Previous endocrine studies indicated large differences women. The predictive values 17-hydroxyprogesterone (17-OHP) response to ACTH stimulation and HLA typing screening for carrier status were re-evaluated. DESIGN: Molecular analysis CYP21 gene, human leucocyte antigen (HLA) performed 83 consecutive MEASUREMENTS: Cortisol 17-OHP concentrations measured baseline 60 min after...
A 47‐year‐old white male patient who manifested biochemical evidence of iron overload was found not to be a carrier the three most common mutations, C282Y, H63D and S65C, HFE gene. Sequencing patient’s entire ‐coding region revealed presence previously undescribed frameshift deletion c.471del in exon 3 resulting premature termination nonsense protein. Interestingly, homozygous this novel mutation his hemochromatosis phenotype can explained by fact that he has no intact To best our knowledge,...
Within the framework of EU-funded HLA-NET action, an analysis three G-group alleles, HLA-B*44:02:01G, DRB1*14:01:01G and DQB1*03:01:01G, was undertaken in 12 European populations. Ambiguities were resolved by polymerase chain reaction-sequence-specific amplification (PCR-SSP) or PCR-sequence-based typing (PCR-SBT) a total 5095 individuals. The results DRB1*14:01/14:54 ambiguity showed high relative ratios (24-53%) DRB1*14:01 Bulgarians, Croatians, Greeks, Italians Slovenians, contrasting...
Abstract The Major Histocompatibility Complex (MHC) is a large gene family that found in most vertebrates and has an important influence on body odour preference mate selection animals. In this research we found, human leukocyte antigen (HLA) phenotype strongly connected with the strength pleasantness of perceived selected chemical compounds sweat. Among different classes tested, esters fatty acids such as methyl undecanoate, decanoate, nonanoate, octanoate hexanoate show strongest...
Summary A novel allele HLA‐B*2730 and the haplotype HLA‐A*03‐B*2730‐Cw*02‐DRB1*16‐DRB5 have been found in a Slovene patient his mother. The exon 2 sequence is identical to that of HLA‐B*2702, 3 HLA‐B*2719. possible mechanism for generation B*2730 gene conversion event.
HLA‐B44 is among the most frequent class I antigens in many populations studied so far. It has been subdivided into seven allelic forms that can only be discriminated by DNA typing. Using a simple PCR/sequence‐specific oligonucleotide hybridization procedure, we have analysed frequency distribution of B44 subtypes three European from Slovenia, Netherlands, and Switzerland. B*4402 B*4403 were far predominant alleles, B*4404 4405 rare, while B*4406 B*4407 not observed. Interestingly, 4403...
Summary A combination of specific HLA class II antigens and the presence type 1 diabetes (T1D)‐related antibodies has a high positive predictive value for T1D but low sensitivity. The aim present study was to determine frequencies HLA‐DRB‐DQB deduced haplotypes associated with susceptibility protection in Slovenian patients established T1D, evaluate relationship between HLA‐DRB1‐QBP‐DQB1 insulin autoantibodies (IAA) glutamic acid decarboxylase (GADA), access possible impact polymorphic QBP...