A5004263912- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Immunodeficiency and Autoimmune Disorders
- Immune responses and vaccinations
- Renal Transplantation Outcomes and Treatments
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Mycobacterium research and diagnosis
- Lymphoma Diagnosis and Treatment
- Immune Response and Inflammation
- Organ Transplantation Techniques and Outcomes
- Diabetes and associated disorders
- Hepatitis C virus research
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- CAR-T cell therapy research
- Organ Donation and Transplantation
- Hepatitis B Virus Studies
- melanin and skin pigmentation
- Virus-based gene therapy research
- Genital Health and Disease
- Prion Diseases and Protein Misfolding
Gartnavel General Hospital
2010-2023
University of Glasgow
2019-2022
NHS Greater Glasgow and Clyde
2006-2019
The Royal Free Hospital
2001-2016
Anthony Nolan
2002-2016
University College London
2001-2016
Innsbruck Medical University
2016
Churchill Hospital
1998
Hôpital Nestlé
1998
Imperial College London
1998
Whole genome comparisons identified introgression from archaic to modern humans. Our analysis of highly polymorphic human leukocyte antigen (HLA) class I, vital immune system components subject strong balancing selection, shows how humans acquired the HLA-B*73 allele in west Asia through admixture with called Denisovans, a likely sister group Neandertals. Virtual genotyping Denisovan and Neandertal genomes HLA haplotypes carrying functionally distinctive alleles that have introgressed into...
The HLA-A,B negative mutant cell line C1R is widely used as a transfection recipient in functional studies of class I MHC genes. It was derived from normal B line, Hmy2, by three rounds mutagenesis and immunoselection with anti-HLA mAb. Serology characterizes to be for the HLA-A2, A3, B35, Bw62, Cw3 Ag parental while retaining expression HLA-Cw4. We find, however, that CTL specific HLA-B35 lyse cells, suggesting also retained. To resolve this paradox we examined HLA-A,B,C genes proteins...
Natural killer cells are a key component in the immune control of viral infections. Their functions controlled by inhibitory receptors for major histocompatability complex (MHC) class I, including cell immunoglobulin-like (KIR). KIR2DL3 combination with its cognate human leukocyte antigen (HLA)-C ligand has been shown to be associated spontaneous resolution viremia following hepatitis C virus (HCV) infection. In order determine if this gene is advantageous across all potential outcomes HCV...
The adenovirus (Ad) early transcription unit 3 (E3) encodes multiple immunosubversive functions that are presumed to facilitate the establishment and persistence of infection. Indeed, capacity E3/19K inhibit transport HLA class I (HLA-I) cell surface, thereby preventing peptide presentation CD8(+) T cells, has long been recognized as a paradigm for viral immune evasion. However, HLA-I downregulation potential render Ad-infected cells vulnerable natural killer (NK) recognition. Furthermore,...
A proportion of classical Hodgkin lymphoma (HL) is believed to be causally related infection with the ubiquitous lymphotropic EBV. The determining factors for development EBV-related HL remain poorly understood, but likely involve immunological control viral infection. Accordingly, markers HLA class I region have been associated risk HL. To study host genetic component further, we investigated lymphoma's association HLA-A*01 and HLA-A*02 simultaneously in setting infectious mononucleosis...
Polymorphisms in the interleukin-28B (IL28B) gene are associated with outcomes from infection hepatitis C virus (HCV). However, role of these polymorphisms protecting injection drug users who at high risk for HCV but do not have detectable antibodies against or RNA (exposed uninfected) has been demonstrated. We investigated whether individuals IL28B genotype rs12979860-CC, which protects some infection.Seventy-four exposed uninfected individuals, 89 spontaneous resolvers, and 234 chronically...
A monomoprhic monoclonal antibody (LA45 antibody) reactive with "a new activation-induced surface structure on human T lymphocytes" antigen) that resembled free class I heavy chains has recently been described (Schnabl, E., H. Stockinger, O. Majdic, Gaugitsch, I.J.D. Lindley, D. Maurer, A. Hajek-Rosenmayr, and W. Knapp. 1990. J. Exp. Med. 171:1431). This was used to clone a I-like chain gene) from the HUT 102 tumor cell, which paradoxically did not give rise LA45 antigen transfection into...
Abstract A review of the British Society for Histocompatibility and Immunogenetics (BSHI) Guideline ‘HLA matching donor selection haematopoietic progenitor cell transplantation’ published in 2016 was undertaken by a BSHI appointed writing committee. Literature searches were performed data extracted presented as recommendations according to GRADE nomenclature.
Nucleotide sequences were determined for the HLA‐A, B and C alleles of three populations Amerindians: Havasupai tribe from North America, Guarani Kaingang tribes South America. All 15 are found in Eastern Hemisphere populations, whereas each have six that appear to be present only Western Hemisphere. Nine “new” come HLA‐B, one comes HLA‐A HLA‐C: ten result recombination point substitution. Of 14 16 alleles, four held common. Despite their differences, possess comparable numbers HLA class I...
Background A stored cord blood donation may be a valuable source of hemopoietic stem cells for allogeneic transplantation when matched sibling donor is not available. We carried out study to define the optimal size national bank UK.Design and Methods calculated actual numbers possible donors chance finding at least one 2,000 unselected 722 non-North Western European patients whom searches had been initiated as function three levels HLA matching (4, 5 6 alleles by HLA-A, -B low -DRB1 high...
Summary HLA‐NET (a European COST Action) aims at networking researchers working in bone marrow transplantation, epidemiology and population genetics to improve the molecular characterization of HLA genetic diversity human populations, with an expected strong impact on both public health fundamental research. Such improvements involve finding consensual strategies characterize populations samples report typings ambiguities; proposing user‐friendly access databases computer tools defining...
Delayed graft function is a prevalent clinical problem in renal transplantation for which there no objective system to predict occurrence advance. It can result significant increase the necessity hospitalisation post-transplant and risk factor other complications.The importance of microRNAs (miRNAs), specific subclass small RNA, have been clearly demonstrated influence many pathways health disease. To investigate miRNAs on allograft performance post-transplant, expression panel...
Abstract We describe for the first time high‐resolution profiling of HLA‐A, ‐B, ‐C, ‐DRB1, ‐DQB1 and ‐DPB1 in a culturally geographically distinct Mexican ethnic group, Tarahumaras. The alleles most frequently found by reference strand‐mediated conformational analysis this population were class I: HLA‐A*240201, *020101/09, *0206, *310102, *680102; HLA‐B*4002, *1501, *510201, *3501/02/03, *4005, *4801; HLA‐Cw*0304, *0801, *0102, *040101; II: HLA‐DRB1*080201, *1402, *040701; HLA‐DQB1*0402,...
HLA genotyping and genome wide association studies provide strong evidence for associations between Human Leukocyte Antigen (HLA) alleles classical Hodgkin lymphoma (cHL). Analysis of these is complicated by the extensive linkage disequilibrium within major histocompatibility region recent data suggesting that with EBV-positive EBV-negative cHL are largely distinct. To distinguish independent therefore potentially causal from confounded disequilibrium, we applied a variable selection...
Abstract: The nucleotide sequence of cDNA encoding the HLA‐B73 antigen was determined; it is unusually divergent, differing from other HLA‐B alleles by 44–77 substitutions. Features that distinguish B*7301 heavy chain chains include multiple substitutions in α 3 domain and a duplication‐deletion within transmembrane region increases length compared to chains. shared with subsets B homologous gorilla (Gogo‐B) chimpanzee (Patr‐B) loci. Other unusual features are individually certain HLA‐A,...
Various HLA-B molecules exhibit serologic cross-reactions with HLA-B7, including HLA-B27, B40, Bw42, and Bw22. Of this group, primary structures for the three subdivisions of HLA-Bw22, HLA-Bw54, Bw55, Bw56, have yet to be determined. Here, we describe nucleotide sequences five distinctive HLA-Bw22 alleles isolated from cells different ethnic origins typed either Bw54, or Bw56. Heterogeneity in as Bw55 Bw56 was defined. The form a closely related family that appears evolved by series simple...
Cell lines RPMI 8226, JJN3, U266 B1, NCI‐H929 (all EBV − ) and ARH77 HS‐Sultan (both + have been extensively characterized in this study. expressed the phenotype (CD138 , CD19 CD20 whereas were ). CD56 expression was restricted to cell lines, with exception of PCA‐1 strongly on five six lines. Only bound peanut‐agglutinin (PNA). However, all lectin Jacalin that binds same receptor as PNA, irrespective receptors sialylation status. By RT‐PCR direct sequencing their IgH V/D/J domains,...
Abstract: A comparison of serological and DNA HLA class I typing data identified a “blank” HLA‐B15 antigen in volunteer donor on the bone marrow registry. Isoelectric focusing Western blot analysis cell line established from this individual confirmed that is not expressed at surface. Nucleotide sequence HLA‐B*15 null allele revealed 10‐bp deletion near 3′ end intron 1, when compared to normal HLA‐B*1501 sequence. All specific cDNA clones examined retained 1 Reverse transcription‐polymerase...
CD8+ T-cell responses to hepatitis C virus (HCV) are important in generating a successful immune response and spontaneously clearing infection. Human leukocyte antigen (HLA) class I presents viral peptides T cells permit detection of infected cells, tapasin is an component the peptide loading complex for HLA I. We sought determine if polymorphisms affected outcome HCV Patients with resolved or chronic infection were genotyped known G/C coding polymorphism exon 4 gene. In European, but not...