A5042051991- Vasculitis and related conditions
- Diabetes Treatment and Management
- Chronic Kidney Disease and Diabetes
- Renal Diseases and Glomerulopathies
- Pancreatic function and diabetes
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Otitis Media and Relapsing Polychondritis
- Sinusitis and nasal conditions
- Neuropeptides and Animal Physiology
- MicroRNA in disease regulation
- Dialysis and Renal Disease Management
- Blood Pressure and Hypertension Studies
- Receptor Mechanisms and Signaling
- Pancreatic and Hepatic Oncology Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Atherosclerosis and Cardiovascular Diseases
- Platelet Disorders and Treatments
- Genetic Syndromes and Imprinting
- Diabetes Management and Research
- Cell Adhesion Molecules Research
- Medication Adherence and Compliance
- Advanced biosensing and bioanalysis techniques
- Diet and metabolism studies
- Genetic and Kidney Cyst Diseases
- Organ Transplantation Techniques and Outcomes
Innsbruck Medical University
2015-2024
Hospital Universitario Fundación Jiménez Díaz
2023
Universidad Autónoma de Madrid
2023
Umeå University
2023
Skaraborg Hospital
2023
Hypertension Institute
2023
Mosaiques Diagnostics and Therapeutics (Germany)
2023
Toronto General Hospital
2023
University of Toronto
2023
Klinikum St. Georg
2023
The sodium-glucose cotransporter 2 (SGLT2) inhibitor canagliflozin slows progression of kidney function decline in type diabetes. aim this study was to assess the effect SGLT2 on biomarkers for diabetic disease (DKD).A mechanism action (MoA) network model constructed based an vitro transcriptomics experiment human proximal tubular cells and molecular features linked inhibitors from scientific literature. This mapped onto established DKD that describes processes associated with DKD....
MicroRNAs (miRNAs) contribute to chronic kidney disease (CKD) progression via regulating mRNAs involved in renal homeostasis. However, their association with clinical outcome remains poorly understood.We performed miRNA and mRNA expression profiling on biopsy sections by qPCR (miRNA) microarrays (mRNA) a discovery (n = 43) validation 29) cohort. miRNAs differentiating stable progressive cases were inversely correlated putative target mRNAs, which further characterized pathway analysis using...
Severe infections contribute to morbidity and mortality in antineutrophil cytoplasm antibody-associated vasculitis (AAV). This study aimed identify risk factors associated with severe participants of the Rituximab versus Cyclophosphamide for ANCA-Associated Vasculitis (RAVE) trial.Data on 197 patients recruited into RAVE trial were analysed. Participants received either rituximab (RTX) or cyclophosphamide (CYC), followed by azathioprine (AZA). Clinical laboratory data without (≥grade 3,...
Abstract Background Risk of kidney function decline in immunoglobulin A (IgA) nephropathy (IgAN) is significant and may not be predicted by available clinical histological tools. To serve this unmet need, we aimed at developing a urinary biomarker-based algorithm that predicts rapid disease progression IgAN, thus enabling personalized risk stratification. Methods In multicentre study, urine samples were collected 209 patients with biopsy-proven IgAN. Progression was defined tertiles the...
Large clinical trials demonstrated that SGLT2 inhibitors (SGLT2i) slow the progression of kidney function decline in type 2 diabetes. Because underlying molecular mechanisms are largely unknown, we studied effects SGLT2i on gene expression two human proximal tubular (PT) cell lines under normoglycemic conditions, utilizing SGLT2i, namely empagliflocin and canagliflocin. Genome-wide analysis did not reveal substantial differences between these SGLT2i. Microarray hybridization identified 94...
Abstract Aims/hypothesis Non-adherence to medication is a frequent barrier in the treatment of patients with type 2 diabetes mellitus, potentially limiting effectiveness evidence-based treatments. Previous studies have mostly relied on indirect adherence measures analyse outcomes based adherence. The aim this study was use LC-MS/MS urine—a non-invasive, direct and objective measure—to assess non-adherence cardiometabolic drugs its association kidney cardiovascular outcomes. Methods This...
Expression of angiogenic cytokines like vascular endothelial growth factor is enhanced by hypoxia. We tested the hypothesis that decreased oxygen levels up-regulate secretoneurin. In vivo, muscle cells mouse ischemic hind limbs showed increased secretoneurin expression, and inhibition a neutralizing antibody impaired response in this ischemia model. soft tissue model hypoxia, was subcutaneous fibers. vitro, mRNA protein were up-regulated L6 myoblast after exposure to low levels. The...
To assess potential associations for the development of venous thromboembolic events in patients with ANCA-associated vasculitides (AAV).Four hundred and seventeen enrolled to participate randomized controlled trials conducted by European Vasculitis Society were identified. Univariate multivariate analyses performed validate previously proposed identify novel risks associated thromboembolism (VTE) AAV.VTE occurred 41 417 (9.8%) patients. Uncorrected univariate analysis identified BVAS (odds...
Abstract Mechanisms underlying the onset and progression of nephropathy in diabetic patients are not fully elucidated. Deregulation proteolytic systems is a known path leading to disease manifestation, therefore we hypothesized that proteases aberrantly expressed (DN) may be involved generation DN-associated peptides urine. We compared urinary peptide profiles DN (macroalbuminuric, n = 121) with no evidence (normoalbuminuric, 118). 302 sequenced, differentially (adjusted p-value < 0.05)...
The prevalence of diabetes mellitus type 2 and kidney disease in these patients varies widely between European countries.In addition to store bio-samples the "Prospective cohort study with for validation biomarkers" collects information on history, physical status, laboratory measurements medication 4000 2, being taken care at primary level healthcare 5 countries (Austria, Hungary, Netherlands, Poland Scotland). Next comparing rate loss eGFR countries, a further objective PROVALID is...
Delayed graft function is a prevalent clinical problem in renal transplantation for which there no objective system to predict occurrence advance. It can result significant increase the necessity hospitalisation post-transplant and risk factor other complications.The importance of microRNAs (miRNAs), specific subclass small RNA, have been clearly demonstrated influence many pathways health disease. To investigate miRNAs on allograft performance post-transplant, expression panel...
To assess the frequency of venous thromboembolism (VTE) events in Rituximab Antineutrophil Cytoplasmic Antibody (ANCA)-Associated Vasculitis (RAVE) trial and identify novel potential risk factors.VTE 197 patients enrolled RAVE were analyzed. Baseline demographic clinical characteristics recorded, univariate multivariate analyses performed to factors associated with VTE ANCA-associated vasculitis (AAV).VTE occurred 16 (8.1%) an overall average time event 1.5 months (range 1.0-2.75). In...
SGLT2 inhibitor-related nephroprotection is—at least partially—mediated by anti-inflammatory drug effects, as previously demonstrated in diabetic animal and human studies, well hyperglycemic cell culture models. We recently presented first evidence for potential of empagliflozin (Empa) under normoglycemic conditions proximal tubular cells (HPTC) demonstrating Empa-mediated inhibition IL-1β-induced MCP-1/CCL2 ET-1 expression on the mRNA protein level. now add corroborating a genome-wide level...
The disease trajectory of diabetic kidney (DKD) shows a high interindividual variability not sufficiently explained by conventional risk factors. Clonal hematopoiesis indeterminate potential (CHIP) is proposed novel cardiovascular factor. Increased fibrosis and glomerulosclerosis were described in mouse models CHIP. Here, we aim to analyze whether CHIP affects the incidence or progression DKD.A total 1419 eligible participants PROVALID Study basis for nested case-control (NCC) design. A 64...
Abstract Background Ear, nose and throat involvement in granulomatosis with polyangiitis (GPA) is frequently the initial disease manifestation. Previous investigations have observed a higher prevalence of Staphylococcus aureus patients GPA, chronic nasal carriage has been linked an increased risk relapse. In this cross-sectional study, we investigated changes microbiota including detailed analysis spp. by shotgun metagenomics active inactive (GPA). Shotgun metagenomic sequence data were also...
Homeostatic mechanisms to maintain the T cell compartment diversity indicate an ongoing process of thymic activity and peripheral renewal during human life. These processes are expected be accelerated after childhood thymectomy by influence cytomegalovirus (CMV) inducing a prematurely aged immune system. The study aimed investigate proportional changes replicative history CD8+ cells, recent emigrants (RTEs) CD103+ cells (mostly gut-experienced) role Interleukin-(IL)-7 IL-7 receptor...
SGLT2 inhibitors (SGLT2i) slow the progression of chronic kidney disease; however, evidence for underlying molecular mechanisms is scarce. We investigated SGLT2i-mediated effects on differential gene expression in two independent human proximal tubular cell (HPTC) lines (HK-2 and RPTEC/TERT1) at mRNA protein levels under normoglycemic conditions, utilizing IL-1β as a pro-inflammatory mediator. Microarray hybridization identified 259 genes that were uniformly upregulated by (10 mg/mL)...
The number of patients with type 2 diabetes mellitus and mellitus–associated chronic kidney disease varies considerably between countries. Next to differences in genetic as well life style risk factors, varying practices medical care delivery might cause this diversity.
Immunoglobulin A nephropathy (IgAN) frequently leads to kidney failure. The urinary proteomics-based classifier IgAN237 may predict disease progression at the time of biopsy. We studied whether also predicts later in course IgAN.Urine from patients with biopsy-proven IgAN was analyzed using capillary electrophoresis-mass spectrometry baseline (IgAN237-1, n = 103) and follow-up (IgAN237-2, 89). Patients were categorized as "non-progressors" (IgAN237 ≤0.38) "progressors" >0.38). Estimated...
Novel prognostic markers for progression of kidney disease are needed to distinguish patients who might benefit from a more aggressive nephroprotective therapy. Expression the proteoglycan versican was evaluated in renal transcriptomics profiles and an independent set 74 biopsies. Versican levels were correlated histologic damage scores outcome, expression regulation vitro. In tissue positively with (i) histological parameters biopsies, (ii) progressive decline function proteinuric diseases,...