A5082295672- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Complement system in diseases
- Monoclonal and Polyclonal Antibodies Research
- Mesenchymal stem cell research
- Renal Transplantation Outcomes and Treatments
- Chronic Myeloid Leukemia Treatments
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Viral Infections and Vectors
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Organ Transplantation Techniques and Outcomes
- Chronic Lymphocytic Leukemia Research
- Blood groups and transfusion
- Proteoglycans and glycosaminoglycans research
- Pluripotent Stem Cells Research
- Eosinophilic Disorders and Syndromes
- Renal Diseases and Glomerulopathies
- Drug Transport and Resistance Mechanisms
- Virus-based gene therapy research
Finnish Red Cross
2009-2025
Åbo Akademi University
2003
University of Turku
2003
Lund University
2003
Turku University Hospital
2003
Somatic mutations contribute to tumorigenesis. Although these occur in all proliferating cells, their accumulation under non-malignant conditions, such as autoimmune disorders, has not been investigated. Here, we show that patients with newly diagnosed rheumatoid arthritis have expanded CD8+ T-cell clones; 20% (5/25) of T but CD4+ harbour somatic mutations. In healthy controls (n=20), only one mutation is identified the pool. Mutations exist exclusively effector-memory subset, persist during...
Abstract Human cord blood (CB)–derived CD133+ cells carry characteristics of primitive hematopoietic and proffer an alternative for CD34+ in stem cell (HSC) transplantation. To characterize the population on a genetic level, global expression analysis was performed using oligonucleotide microarrays. were purified from four fresh CB units by immunomagnetic selection. All samples showed significant similarity their gene pattern, whereas they differed clearly control samples. In all, 690...
Complex carbohydrate structures, glycans, are essential components of glycoproteins, glycolipids, and proteoglycans. While individual glycan structures including the SSEA Tra antigens already used to define undifferentiated human embryonic stem cells (hESC), whole spectrum cell glycans has remained unknown. We undertook a global study asparagine-linked glycoprotein (N-glycans) hESC their differentiated progeny using MALDI-TOF mass spectrometric NMR spectroscopic profiling. Structural...
When preparing stem cell specimens from cord blood, pre-enrichment of mononuclear cells is highly recommended to improve the recovery rare cells. Mononuclear are easily isolated by density gradient centrifugation. In Ficoll-Paque centrifugation, anticoagulant-treated and diluted blood layered on solution centrifuged. During erythrocytes granulocytes sediment bottom layer. Lower lymphocytes, together with other slowly sedimenting such as platelets monocytes, retained at interface between...
Immune aplastic anemia (AA) is a life-threatening bone marrow failure disorder driven by an autoimmune T cell attack against hematopoietic stem and progenitor cells (HSPCs). However, the exact autoantigen targets role of other immune in pathogenesis AA are unknown. Here, we analyzed cohort 218 patients with using single-cell RNA receptor (TCR) αβ sequencing, TCRβ flow cytometry, plasma cytokine profiling. We identified natural killer (NK) CD8 + terminally differentiated effector (T EMRA )...
There is a growing interest in cord blood as source of primitive stem cells with the capacity for multilineage differentiation. Pure cell fractions are needed characterization and vitro expansion well their use preclinical research. However, enrichment challenging due to lack cell-specific markers gentle protocols isolation highly pure fractions. Protocols developed peripheral blood-derived have been found be suboptimal blood.In this study, immunomagnetic sorting purify CD34+, CD133+ Lin-...
Abstract Somatic stem cell transplantation holds great promise in regenerative medicine. The best-characterized adult cells are mesenchymal (MSCs), neural (NSCs), and CD133+ hematopoietic (HSCs). applications of HSCs hampered since these difficult to maintain an undifferentiated state vitro. Understanding genes responsible for properties their interactions will help on this issue. construction genetic networks also develop rational strategies revert somatic back a stem-like state. We...
Xanthine oxidase inhibitors febuxostat and allopurinol are commonly used in the treatment of gout. Febuxostat inhibits breast cancer resistance protein (BCRP) vitro. Rosuvastatin is a BCRP substrate genetic variability markedly affects rosuvastatin pharmacokinetics. In this study, we investigated possible effects on randomized crossover study with 3 phases, 10 healthy volunteers ingested once daily placebo for 7 days, 300 mg or followed by 120 4 single dose day 6. increased peak plasma...
Summary We present here the results of Analysis HLA Population Data ( AHPD ) project 16th International and Immunogenetics Workshop (16 IHIW held in Liverpool May–June 2012. Thanks to collaboration 25 laboratories from 18 different countries, genotypic data for 59 new population samples (either well‐defined populations or donor registry samples) were gathered 55 analysed statistically following ‐ NET recommendations. The included, among others, large sets north‐east Europe West Asia, as well...
Long cold ischemia time (CIT) is the most important factor contributing to delayed graft function (DGF) after kidney transplant. Improvements in pretransplant procedures may reduce CIT and improve clinical outcome.Pretransplant histocompatibility tests were modernized at our laboratory 2015, leading significant decrease of consumed for these enabling earlier surgery. The effects this on transplant CIT, DGF, other outcomes studied. study population consisted 896 consecutive deceased donor...
Abstract Immunological control of residual leukemia cells is thought to occur in patients with chronic myeloid (CML) that maintain treatment-free remission (TFR) following tyrosine kinase inhibitor (TKI) discontinuation. To study this, we analyzed 55 single-cell RNA and T cell receptor (TCR) sequenced samples (scRNA+TCRαβ-seq) from CML ( n = 13, N 25), other cancers 28), healthy 7). The high number active phenotype natural killer (NK) separated them cancers. Most NK belonged the CD56 dim...
Abstract Human stem cells contain substantial amounts of the xenoantigen N-glycolylneuraminic acid (Neu5Gc), although levels Neu5Gc are low or undetectable in human body fluids and most other tissues. The lack tissues has been previously explained by loss hydroxylase activity CMP-N-acetylneuraminic (CMAH) protein caused a genetic error Cmah gene. We thus wanted to investigate whether redundant gene could still function cell-specific processes. In this study, we show that CMAH expression is...
Abstract The human leukocyte antigen ( HLA ) antigen, allele and haplotype frequencies of the Finnish population are quite unique because a rather restricted homogeneous gene pool. This has strong influence on finding suitable donors for transplant patients; hence knowledge about patient is essential. Here we report large sample show high resolution 11 loci, including rarely typed DPA1 DQA1 loci. Furthermore, most common haplotypes presented five study shows that there fewer in compared with...
CD34 and CD133 are the most commonly used markers to enrich hematopoietic stem cells (HSCs). Positively selected HSCs increasingly for autologous allogeneic transplantation, yet biological properties of CD34(+) CD133(+) largely unknown. In present study, a genome-wide gene expression analysis human cord blood (CB)-derived was performed. The profile compared an identically constructed reveal specific patterns major differences cells. As expected, many genes were similarly expressed in two...
Graft-versus-host disease (GvHD) is a major complication in hematopoietic stem cell transplantation (HSCT). The immune response against gut microbes thought to be an important factor the beginning of GvHD. Toll-like receptors (TLR) recognize molecular structures and viruses play central part innate immunity. We studied whether genetic variation TLR1, TLR2, TLR4, TLR5, TLR6 TLR10 genes confers susceptibility GvHD 305 human leucocyte antigen-identical sibling donor HSCT's performed single...
In immune aplastic anemia (IAA), severe pancytopenia results from the immune-mediated destruction of hematopoietic stem cells. Several autoantibodies have been reported, but no clinically applicable autoantibody tests are available for IAA. We screened using a microarray containing >9000 proteins and validated findings in large international cohort IAA patients (n = 405) controls 815). identified novel that binds to C-terminal end cyclooxygenase 2 (COX-2, aCOX-2 Ab). total, 37% all adult...
Enrichment of hematopoietic stem cells is based on the expression certain surface antigens, such as CD34 and CD133, or lack lineage-specific antigens. Immunomagnetic positive selection CD34(+) CD133(+) performed using paramagnetic microbeads conjugated to specific monoclonal antibodies (anti-human anti-human CD133). In negative lineage-negative (Lin(-)) cells, unwanted are labeled with against known markers for mature (CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD66b, glycophorin A) retained in...
Protocol biopsies are used to monitor allograft histology after transplantation. However, biopsy is an invasive procedure with potential complications, requires special facilities, and unpractical for repeated monitoring of the graft. A noninvasive, robust, rapid diagnostic method would be welcomed. Monitoring gene expression from blood samples could provide such a means.Whole taken at time 3- or 6-month protocol in 31 pediatric renal transplant recipients, 13 whom had biopsy-proven...
Within the framework of EU-funded HLA-NET action, an analysis three G-group alleles, HLA-B*44:02:01G, DRB1*14:01:01G and DQB1*03:01:01G, was undertaken in 12 European populations. Ambiguities were resolved by polymerase chain reaction-sequence-specific amplification (PCR-SSP) or PCR-sequence-based typing (PCR-SBT) a total 5095 individuals. The results DRB1*14:01/14:54 ambiguity showed high relative ratios (24-53%) DRB1*14:01 Bulgarians, Croatians, Greeks, Italians Slovenians, contrasting...
Loss of heterozygosity (LOH) has been reported to cause false human leukocyte antigen (HLA) homozygous typing results in pre-transplant patients suffering from haematological malignancies, who fact are HLA heterozygous. This poses a challenge for histocompatibility testing, as stem cell graft genuinely donor mistyped patient may lead acute life-threatening graft-vs-host disease. LOH the region on chromosome 6 is known be quite common solid tumours, helping malignant cells escape T-cell...
ABSTRACT Deficiencies of the early components classical complement pathway impair actions innate and humoral immunity may lead to increased susceptibility infections. We have studied genetic basis total C4B deficiency in a Finnish patient with recurrent meningitis, chronic fistulas abscesses. The maternal chromosome carried four-gene deletion including gene, conversion from C4A gene was found on paternal resulting complete C4B. In converted mutation screening did not reveal any amino acid...