A5027056707- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Cellular transport and secretion
- Micro and Nano Robotics
- Model Reduction and Neural Networks
- Fungal and yeast genetics research
- Numerical methods for differential equations
- Advanced Fluorescence Microscopy Techniques
- Fungal Biology and Applications
- 14-3-3 protein interactions
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- Advanced Electron Microscopy Techniques and Applications
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Congenital heart defects research
- Hemoglobin structure and function
- Nuclear Receptors and Signaling
- Macrophage Migration Inhibitory Factor
- Genetics, Aging, and Longevity in Model Organisms
- Diffusion and Search Dynamics
- Morphological variations and asymmetry
University of Münster
2015-2024
Stanford University
2008-2014
Augusta University
2004-2008
University of Ljubljana
2004-2008
Institute of Oncology Ljubljana
2000
Planar cell polarity (PCP) signaling controls the polarization of cells within plane an epithelium. Two molecular modules composed Fat(Ft)/Dachsous(Ds)/Four-jointed(Fj) and a ‘PCP-core’ including Frizzled(Fz) Dishevelled(Dsh) contribute to individual cells. How is globally coordinated with tissue axes unresolved. Consistent previous results, we find that Ft/Ds/Fj-module has effect on MT-cytoskeleton. Here, provide evidence for model provides directional information core-module through this...
Abstract When the brain is in a pathological state, content of lipid droplets (LDs), storage organelles, increased, particularly glial cells, but rarely neurons. The biology and mechanisms leading to LD accumulation astrocytes, cells with key homeostatic functions, are poorly understood. We imaged fluorescently labeled LDs by microscopy isolated tissue rat astrocytes glia‐like Drosophila determine (sub)cellular localization, mobility, under various stress conditions characteristic for...
Microtubules (MTs) are substrates upon which plus- and minus-end directed motors control the directional movement of cargos that essential for generating cell polarity. Although centrosomal MTs organized with plus-ends away from MT organizing center, regulation non-centrosomal polarity is poorly understood. Increasing evidence supports model information planar polarization derived alignment a parallel apical network MT-dependent trafficking downstream signaling components. The...
In the vertebrate central nervous system, exploratory filopodia transiently form on dendritic branches to sample neuronal environment and initiate new trans-neuronal contacts. While much is known about molecules that control extension subsequent maturation into functional synapses, mechanisms regulate initiation of these dynamic, actin-rich structures have remained elusive. Here, we find suppressed by recruitment ArhGAP44 actin-patches seed filopodia. Recruitment mediated binding a membrane...
Abstract In order to shape a tissue, individual cell-based mechanical forces have be integrated into global force pattern. Over the last decades, importance of actomyosin contractile arrays, which are key constituents various morphogenetic processes, has been established for many tissues. Recent studies demonstrated that microtubule cytoskeleton mediates folding and elongation epithelial sheet during Drosophila morphogenesis, placing mechanics on par with actin-based processes. While these...
Abstract In vivo , cells experience complex tissue environments with various chemical and physical features. They sense respond to morphology mechanical properties adjust their behavior function based on the surrounding. contrast free environment experienced 2D substrates commonly used in research, 3D natural represents a major obstacle for cells. Here, are usually confined either by extracellular matrix (ECM) or neighboring The importance of such confinements has been demon-strated past...
Filopodia are dynamic, actin-rich structures that transiently form on a variety of cell types. To understand the underlying control mechanisms requires precise monitoring localization and concentration individual regulatory structural proteins as filopodia elongate subsequently retract. Although several methods exist analyze changes in filopodial shape, software solution to reliably correlate growth dynamics with spatially resolved protein along filopodium independent bending, lateral shift,...
Tandem mass spectrometry is a method of choice for rapid analysis in proteomics. Identification and characterization proteins from organisms with sequenced genomes today routine procedure as will be identification unsequenced new developing tools. Here, we report the use isotopic labeling electrospray ionisation (ESI)-tandem de novo sequencing combination database search taking advantage different programs fungal proteins. Using this approach could identify interest. Nevertheless, novel...
Ammodytoxin (Atx), an sPLA2 (secretory phospholipase A2), binds to g and e isoforms of porcine 14-3-3 proteins in vitro. are evolutionarily conserved eukaryotic regulatory involved a variety biological processes, including cell-cycle regulation. We have now shown that Atx yeast with affinity similar for the mammalian isoforms. Thus Saccharomyces cerevisiae can be used as model cell, which lacks endogenous phospholipases A2, assess vivo relevance this interaction. was expressed cells...
Migrating cells can serve as probes for determining tissue properties in live embryos.
Far-field super-resolution microscopy is a rapidly emerging method that opening up opportunities for biological imaging beyond the optical diffraction limit. We have implemented Stimulated Emission Depletion (STED) microscope to image single dye, cell, and tissue samples with 50-80 nm resolution. First, we compare STED performance molecules of several common dyes report novel dye. Then apply planar cell polarity protein complexes in intact fixed
Force-regulation at cellular membranes relies on dynamic molecular platforms that integrate intra- and extracellular signals to control cell shape function. To correctly respond a continuously changing environment, activity of these needs be tightly controlled in space time. Over the last few years, curvature-dependent mechano-chemical signal translation—a receptor-independent signaling mechanism where physical forces plasma membrane trigger nanoscale deformations are then translated into...
In the developing Drosophila abdomen, epithelial tissue displays extensive cytoskeletal remodeling. stark contrast to spatio-temporal control of actin cytoskeleton, regulation microtubule architecture during morphogenesis has remained opaque. particular, its role in cell motility remains unclear. Here, we show that minus-end binding protein Patronin is required for organizing arrays histoblast cells form abdomen. Loss results a dorsal cleft, indicating compromised function histoblasts. We...
The response of the halophilic black yeast Hortaea werneckii to steroid hormone progesterone has been studied at protein level using fluorescent two-dimensional differential gel electrophoresis (2D-DIGE) technology in combination with mass spectrometry. Data on identification from this study reveal molecular mechanisms progesterone. In particular, overexpression Pck2 and Pac2 stimulated cells indicates interactions cell growth reproduction signaling pathways. Keywords: • de novo sequencing...
Polarization of tissue is achieved by asymmetric distribution proteins and organelles within individual cells. However, existing quantitative assays to measure this asymmetry in an automated unbiased manner suffer from significant limitations.Here, we report a new way assess protein organelle localization based on correlative fluorescence analysis. As proof principle, successfully characterized planar cell polarity dependent developing Drosophila melanogaster tissues the single level using...
SUMMARY Mechanical stimuli, particularly laminar blood flow, play a crucial role in shaping the vascular system. Changes rate of flow manifest altered shear stress, which activates signaling cascades that drive remodeling. Consistently, dysregulation endothelial response to fluid forces and aberrant patterns both lead pathological conditions, including impaired vessel development atherosclerosis. Despite its importance, mechanisms driving coordinated cell behavior underlying remodeling are...