A5073883126- Vasculitis and related conditions
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Systemic Sclerosis and Related Diseases
- Atherosclerosis and Cardiovascular Diseases
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Pancreatitis Pathology and Treatment
- Renal Diseases and Glomerulopathies
- Inflammatory Myopathies and Dermatomyositis
- Bone Tissue Engineering Materials
- Optical Imaging and Spectroscopy Techniques
- Lipoproteins and Cardiovascular Health
- Electrospun Nanofibers in Biomedical Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Urticaria and Related Conditions
- Inflammasome and immune disorders
- Sarcoidosis and Beryllium Toxicity Research
- Otitis Media and Relapsing Polychondritis
- COVID-19 Clinical Research Studies
- Immunodeficiency and Autoimmune Disorders
- Infrared Thermography in Medicine
- Mesenchymal stem cell research
University of Primorska
2016-2025
Ljubljana University Medical Centre
2015-2024
University of Belgrade
2022
ETH Zurich
2019
Northwestern University
2015-2019
University Medical Center
2018
University of Ljubljana
2011-2013
Abstract The factors responsible for maintaining persistent organ fibrosis in systemic sclerosis (SSc) are not known but emerging evidence implicates toll-like receptors (TLRs) the pathogenesis of SSc. Here we show expression, mechanism action and pathogenic role endogenous TLR activators skin from patients with SSc, fibroblasts, mouse models fibrosis. Levels tenascin-C elevated SSc biopsy samples, serum fibrotic tissues mice. Exogenous stimulates collagen gene expression myofibroblast...
Olive leaf extract (OLE) is used in traditional medicine as a food supplement and an over-the-counter drug for variety of its effects, including anti-inflammatory anti-atherosclerotic ones. Mechanisms through which OLE could modulate these pathways human vasculature remain largely unknown. Serum amyloid A (SAA) plays causal role atherosclerosis cardiovascular diseases induces pro-inflammatory pro-adhesive responses coronary artery endothelial cells (HCAEC). Within this study we explored...
Abstract Introduction Progressive fibrosis in systemic sclerosis (SSc) is linked to aberrant transforming growth factor beta (TGF-beta) signaling. Peroxisome proliferator-activated receptor gamma (PPAR-gamma) blocks fibrogenic TGF-beta responses vitro and vivo . Reduced expression function of PPAR-gamma patients with SSc may contribute progression fibrosis. Here we evaluated the levels adiponectin, a sensitive specific index activity, as potential biomarker SSc. Methods Adiponectin were...
Skin fibrosis in systemic sclerosis (SSc) is accompanied by attrition of dermal white adipose tissue (dWAT) and reduced levels circulating adiponectin. Since adiponectin has potent regulatory effects on fibroblasts, we sought to assess signaling SSc skin biopsies, evaluate mice with gain- loss-of-function mutations. Furthermore, investigated the mechanism action agonist peptides targeting receptors vitro vivo. We found that pathway activity was significantly a subset biopsies. Mice lacking...
We present an optimized dissociation protocol for preparing high-quality skin cell suspensions in-depth single-cell RNA-sequencing (scRNA-seq) analysis of fresh and cultured human skin. Our enabled the isolation a consistently high number highly viable cells from small freshly dissociated punch biopsies, which we use scRNA-seq studies. recapitulated not only main populations existing atlases, but also identified rare populations, such as mast cells. Furthermore, effectively isolated single
Efficient stent implantation among others depends on avoiding the aggregation of platelets in blood vessels and appropriate proliferation endothelial cells controlled smooth muscle cells, which reduces development pathology, such as neointimal hyperplasia, thrombosis, restenosis. The current article provides an elegant solution for prevention platelet cell adhesion activation surfaces while obtaining surface conditions to support growth human coronary artery cells. This was achieved by...
Abstract Introduction IgA vasculitis (IgAV) can present as skin-limited or systemic disease, which be severe in adults. Predictive markers for visceral involvement are suboptimal. Considering haptoglobin’s role an acute phase reactant, we evaluated whether its differential expression IgAV patients’ skin and leukocytes is also reflected systemically a larger cohort of adult patients. Additionally, soluble form haptoglobin scavenger receptor CD163 was measured patient serum. Methods We...
Abstract The hallmarks of systemic sclerosis (SSc) are autoimmunity, microangiopathy and fibrosis. Skin fibrosis is accompanied by attrition the dermal white adipose tissue layer, alterations in levels function adiponectin. Since these findings potentially implicate adiponectin pathogenesis SSc, we employed a novel pharmacological approach to augment signaling using AdipoRon, an orally active receptor agonist. Chronic treatment with AdipoRon significantly ameliorated bleomycin-induced mice....
Inflammation in systemic sclerosis (SSc) is a prominent, but incompletely characterized feature early stages of the disease. The goal these studies was to determine circulating levels, clinical correlates and biological effects acute phase protein serum amyloid A (SAA), marker inflammation, patients with SSc. Circulating levels SAA were determined by multiplex assays from 129 SSc 98 healthy controls. Correlations between laboratory features disease analyzed. on human pulmonary fibroblasts...
Early diagnosis and treatment of giant cell arteritis (GCA) is crucial for preventing ischemic complications. Multiple serological markers have been identified; however, there a distinct lack predicting GCA relapse Our main objective was to identify parameters in large cohort treatment-naïve patients, which could support clinicians evaluating the course disease. Clinical data gathered, along with analyte detection using Luminex technology, ELISA, nephelometry, among others. Unsupervised...
Abstract Introduction Immunoglobulin A vasculitis (IgAV) in adults has a variable disease course, with patients often developing gastrointestinal and renal involvement thus contributing to higher mortality. Due understudied molecular mechanisms IgAV currently used biomarkers for visceral are largely lacking. Our aim was search potential serum based on the skin transcriptomic signature. Methods RNA sequencing analysis conducted biopsies collected from 6 treatment-naïve (3 only 3 involvement)...
The present study introduces an advanced surface modification approach combining electrochemical anodization and non-thermal plasma treatment, tailored for biomedical applications on stainless steel grade 316L (SS316L) surfaces. Nanopores with various diameters (100-300 nm) were synthesized anodization, samples further modified oxygen plasma. properties of SS316L surfaces examined by scanning electron microscopy, atomic force X-ray photoemission spectroscopy, Water contact angle...
Background Safe and effective vaccines against COVID-19 are critical for preventing the spread of SARS-CoV-2, but little is known about humoral immune response more than 9 months after vaccination. We aimed to assess first, second, third (booster) doses BNT162b2 vaccine in SARS-CoV-2 naïve previously infected healthcare professionals (HCP) infection vaccinated HCP. Methods measured anti-spike (anti-S) anti-nucleocapsid antibodies at different time points up 12 sera 300 HCP who had received...
Immunoglobulin A vasculitis (IgAV) is an immune complex, small vessel with dominant IgA deposits in walls, predominantly affecting the pediatric population. However, adults frequently have more severe gastrointestinal tract (GIT) and renal involvements as compared to children. Our aim was study serological cellular biomarkers support clinicians their diagnosis course of IgAV adult patients. This cross-sectional included 62 patients 53 healthy blood donors (HBDs). Demographic clinical data,...
Abstract Introduction An international cohort study of 73 anti-Ku-positive patients with different connective tissue diseases was conducted to differentiate the populations based on their autoantibody profile and clinical signs/symptoms establish possible correlations between antibodies against Ku p70 p80 autoimmune diseases. Methods Sera were collected from six European centers all secondarily tested (in reference center); confirmed as positive. Anti-Ku detected...
Significance Oxidative stress, which accompanies sterile inflammation, induces release of extracellular vesicles (stressEVs), activate Toll-like receptor 4 (TLR4), resulting in a pattern gene expression distinct from response triggered by bacterial lipopolysaccharide. The synergy between 15-lipoxygenase and secreted phospholipase A 2 , both are induced is necessary for the formation oxidized lysophospholipids, TLR4 endogenous agonists. Moreover, was sPLA promoted K/BxN serum-induced...
Animal models are invaluable in the research of pathophysiology interstitial cystitis/bladder pain syndrome (IC/BPS), a chronic aseptic urinary bladder disease unknown etiology that primarily affects women. Here, mouse model IC/BPS was induced with multiple low-dose cyclophosphamide (CYP) applications and thoroughly characterized by RNA sequencing, qPCR, Western blot, immunolabeling to elucidate key inflammatory processes sex-dependent differences response. CYP treatment resulted...
Studies on the serum biomarkers of granulomatous inflammation and pulmonary interstitial disease in intrathoracic sarcoidosis have shown conflicting results. We postulated that differences concentrations can be explained by heterogenous patterns seen thoracic HRCT. Serum biomarker levels 79 consecutive patients, newly diagnosed with sarcoidosis, were compared to our control group 56 healthy blood donors. An analysis was performed respect HRCT characteristics (the presence lymph node...