Olive leaf extract (OLE) is used in traditional medicine as a food supplement and an over-the-counter drug for variety of its effects, including anti-inflammatory anti-atherosclerotic ones. Mechanisms through which OLE could modulate these pathways human vasculature remain largely unknown. Serum amyloid A (SAA) plays causal role atherosclerosis cardiovascular diseases induces pro-inflammatory pro-adhesive responses coronary artery endothelial cells (HCAEC). Within this study we explored...

The biological activities, including cell viability, oxidative stress, genotoxicity/antigenotoxicity, and antimicrobial activity, were evaluated for the visible-light-responsive TiO2-based ICT complex with dihydroquercetin (DHQ) compared pristine TiO2, its inorganic component. Pristine TiO2 did not induce cytotoxicity in MRC-5 or HeLa cells within tested concentration range (1-20 mg/mL), while TiO2/DHQ displayed a significant reduction viability both lines at higher concentrations (≥10...

Biological activities, including cell viability, oxidative stress, genotoxicity/antigenotoxicity, and antimicrobial activity, were evaluated for a visible-light-responsive TiO2-based ICT complex with dihydroquercetin (DHQ) compared pristine TiO2, its inorganic component. Pristine TiO2 did not induce cytotoxicity in MRC-5 or HeLa cells within the tested concentration range (1-20 mg/mL), while TiO2/DHQ displayed significant reduction viability both lines at higher concentrations (≥10 mg/mL)....

Hyssopus officinalis L. is a well-known aromatic plant used in traditional medicine and the food cosmetics industry. The aim of this study to assess antioxidant, genotoxic, antigenotoxic cytotoxic properties characterized hyssop essential oils methanol extracts. Chemical composition was analyzed by gas chromatography - mass spectrometry (GC-MS) liquid with diode array detection (LC-DAD-MS), respectively. Antioxidant activity examined 2,2-diphenyl-1-picrylhydrazyl (DPPH) ferric...

The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for antigenotoxic properties the first time. Antigenotoxic effects human peripheral blood cells against H 2 O ‐induced DNA damage were examined pretreatment posttreatment protocol by comet assay. results showed better on interventional level, respectively, after treatment. concentration 250 μ g/mL treatment most efficient regard to action damage. evaluation repair kinetics decrease induced 15 min...

The thyroid hormones change the rate of basal metabolism, modulating consumption oxygen and causing production reactive species, which leads to development oxidative stress DNA strand breaks. Olive ( Olea europaea L.) leaf contains many potentially bioactive compounds, making it one most potent natural antioxidants. objective this study was evaluate genotoxicity L-thyroxine investigate antioxidative antigenotoxic potential standardized oleuropein-rich dry olive extract (DOLE) against...

The health benefits of natural products have long been recognized. Consumption dietary compounds such as supplements provides an alternative source to those obtained from the diet. There is a growing concern regarding possible side effects using different food simultaneously, since their interactions are less known. For first time, we tested genotoxic and antigenotoxic Biochaga, in combination with dihydroquercetin. No effect on whole blood cells was observed within individual treatment...

Abstract Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing stabilizing agents to prepare colloidal 20–25 nm silver nanoparticles (Ag NPs). The Ag NPs characterized using transmission electron microscopy, X-ray diffraction analysis, absorption spectroscopy. cytotoxic actions of coated NPs, their inorganic organic components, examined against trophoblast cells human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, yeast....

Harmful effects of elevated levels catecholamines are mediated by various mechanisms, including gene transcription and formation oxidation products. The aim this study was to see whether the molecular mechanisms underlying damaging action adrenaline on DNA reactive oxygen species (ROS). To do that, we exposed human whole blood cells 10 μmol L-1adrenaline or 50 L-1H2O2(used as positive control) that were separately pre-treated post-treated with 500 L-1of quercetin, a scavenger free radicals....

The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting zirconium dioxide (ZrO2 NPs) dihydroquercetin (DHQ), was performed against Gram (-) bacteria Escherichia coli (+) Staphylococcus aureus, as well human cervical cancer cells HeLa healthy MRC-5 cells. surface modification ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to interfacial charge transfer (ICT) complex formation...

Systemic oxidative stress stemming from increased free radical production and reduced antioxidant capacity are common characteristics of obese individuals. Using hydrogen peroxide (H2O2) to induce DNA damage in vitro, peripheral blood mononuclear cells (PBMCs) subjects controls, the protective ability dihidroqercetin (DHQ) biochaga (B) alone or combination, were evaluated. The effects DHQ B estimated under two experimental conditions: pre-treatment, where pre-incubated with substances prior...

The Comet assay is a highly sensitive and rapid method for detecting DNA damage at the level of individual cells. It measures single-stranded double-stranded breaks, alkali-labile, incomplete excision repair cross-linking sites. A major advantage its applicability to both proliferating non-proliferating cells, it can be performed on all types eukaryotic basic principle alkaline lyse biological membranes release proteins bound DNA, followed by short-term electrophoresis pH above 13. Through...

Despite significant progress in drug discovery and the promising antitumor potential of Combretastatin-A4 (CA-4) selectively targeting rapidly dividing cancer cells, its effects on non-dividing human such as peripheral blood mononuclear cells (PBMCs), remain unclear. This study aims to assess vitro bioactivity CA-4 PBMCs, with a focus antigenotoxic antioxidant properties, while comparing cytotoxic potency against cell lines (JAR HeLa), normal trophoblast line HTR-8/SVneo. Cell viability...

Despite significant advances in drug discovery and the promising antitumor potential of combretastatin A4 (CA-4), which selectively targets rapidly dividing cancer cells, CA-4’s effects on non-dividing human such as peripheral blood mononuclear cells (PBMCs), remain unclear. The aim this study is to evaluate vitro bioactivity CA-4 PBMCs, focusing its antigenotoxic antioxidant properties, while comparing cytotoxic potency against cell lines (JAR HeLa), normal trophoblast line HTR-8/SVneo....

Chromosome instability in Alzheimer’s disease (AD) neurons has been previously reported. This pilot study aimed to establish a quantitative technique for assessing X chromosome centromere signals using fluorescence situ hybridization (FISH). Hippocampal brain tissue was collected at autopsy from sporadic AD patients and age-and gender-matched controls. FISH utilized detect measure the intensity of centromeres interphase nuclei hippocampal cells. The premature division (PCD)...

Abstract Medicinal mushroom extracts, i.e. their dried biomass, have long been known as sources of bioactive compounds with positive effects on the human health. The antioxidant, antigenotoxic, antiviral, and immunomodulatory properties commercially available extracts Agaricus blazei auct. non Murrill (AB), Cordyceps sinensis (Berk.) Sacc. (CS), Immune Assist (IA) already documented. This study, studied influence these three mushrooms viability cell lines MCF-7, MDA-MB-231, HS-5....

The genotoxic and antigenotoxic behavior of the interfacial charge transfer (ICT) complex between nano-sized TiO2 particles caffeic acid (CA) was studied in vitro experiments. formation ICT is indicated by appearance absorption visible-spectral range. continual variations method bridging coordination ligand, acid, surface Ti atoms, while stability constant found to be 1.5?103 mol-1 L. An agreement experimental data theoretical results, based on density functional theory, found. its...

Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) two groups RA patients: the early long-term group; examine potential concomitant treatment with Methotrexate (MTX) Dry olive leaf extract (DOLE) against damage patients after a 3-weeks treatment. A total 32 10healthy individuals included. equally divided into four groups: phase (one treated...