A5060565168- Zebrafish Biomedical Research Applications
- Cellular transport and secretion
- Physiological and biochemical adaptations
- Lysosomal Storage Disorders Research
- Antibiotics Pharmacokinetics and Efficacy
- Marine Sponges and Natural Products
- Retinal Development and Disorders
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Pharmaceutical and Antibiotic Environmental Impacts
- Genetics and Neurodevelopmental Disorders
- Nicotinic Acetylcholine Receptors Study
- Fish biology, ecology, and behavior
- Cellular Mechanics and Interactions
- Computational Drug Discovery Methods
- Pancreatic function and diabetes
- Fish Ecology and Management Studies
- Genetic Neurodegenerative Diseases
- Microbial Natural Products and Biosynthesis
- Environmental Toxicology and Ecotoxicology
- Photoreceptor and optogenetics research
- Cell Adhesion Molecules Research
- Genomics and Rare Diseases
- Cell Image Analysis Techniques
- Animal testing and alternatives
Australian Regenerative Medicine Institute
2021-2024
Monash University
2021-2024
Max Planck Institute for Heart and Lung Research
2019
An early step in pancreas development is marked by the expression of transcription factor Pdx1 within pancreatic endoderm, where it required for specification all endocrine cell types. Subsequently, becomes restricted to β-cell lineage, plays a central role function. This pivotal at various stages makes an attractive target enhance differentiation and increase In this study, we used newly generated zebrafish reporter screen over 8000 small molecules modulators pdx1 expression. We found four...
Mucopolysaccharidosis IIIA (MPS IIIA, Sanfilippo syndrome type A), a paediatric neurological lysosomal storage disease, is caused by impaired function of the enzyme N-sulfoglucosamine sulfohydrolase (SGSH) resulting in catabolism heparan sulfate glycosaminoglycan (HS GAG) and its accumulation tissues. MPS represents significant proportion childhood dementias. This condition generally leads to patient death teenage years, yet no effective therapy exists for complete understanding mechanisms...
Large-scale chemobehavioral phenotyping with zebrafish embryos is a promising avenue for accelerated neurotoxicity testing and discovery of behavior-modifying neuroceuticals. These strategies are hampered by lack effective embryo in-test positioning, wide-field imaging, high-throughput bioinformatic analytics. In this study, we demonstrate advantages using custom large-density arrays in conjunction an open-source ultra-high-definition video imaging system. Moreover, present bioinformatics...
Many targeted natural product isolation approaches rely on the use of pre-existing bioactivity information to inform strategy used for new bioactive compounds. Bioactivity can be available either in form prior assay data or via Structure Activity Relationship (SAR) which indicate a potential chemotype that exhibits desired bioactivity. The work described herein utilizes unique method using structure-based virtual screening identify antibacterial compounds active against MRSA within marine...
Due to increasing numbers of anthropogenic chemicals with unknown neurotoxic properties, there is an need for a paradigm shift toward rapid and higher throughput behavioral bioassays. In this work, we demonstrate application purpose-built high multidimensional test battery on larval stages Danio rerio (zebrafish) at 5 days post fertilization (dpf). The automated comprised the established spontaneous swimming (SS), simulated predator response (SPR), photomotor (LPR) assays as well new...
Chemobehavioural phenotyping presents unique opportunities for analyzing neurotoxicants and discovering behavior-modifying neuroceuticals in small aquatic model organisms such as zebrafish (Danio rerio). A recently popularized approach this field involves the utilization of embryos a photo-motor response (PMR) bioassay. The PMR bioassay entails stimulating between 24 36 h post fertilization (hpf) with high-intensity light stimulus, inducing transient increase frequency photo-induced embryo...
Assessment of animals' sensory–motor functions requires precise and electronically controlled stimuli to induce quantify specific behavioral phenotypes. However, accessible inexpensive tools for conducting diverse biotests with fish are lacking. In this work, we present an open-source software hardware interface that enables automated delivery three independent fully programmable bioassays. We demonstrate the proof-of-concept application low-cost technology in establishing reproducible fear...
Abstract Mucopolysaccharidoses are lysosomal storage diseases that collectively represent a major cause of lethal, treatment-refractory childhood dementias 1–7 Clinically-useful interventions hampered due to an incomplete understanding their neuropathological origins. Using the zebrafish sgsh model mucopolysaccharidosis IIIA 8 (MPS IIIA, Sanfilippo syndrome A), we conducted several ‘omics-based analyses, and developed benchmarked novel bioinformatic feature classification ranking for...