A5027290610- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Sexual Differentiation and Disorders
- Reproductive Health and Technologies
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pluripotent Stem Cells Research
- Testicular diseases and treatments
- Tissue Engineering and Regenerative Medicine
- COVID-19 Impact on Reproduction
- Mosquito-borne diseases and control
- 3D Printing in Biomedical Research
- COVID-19 Clinical Research Studies
- Urological Disorders and Treatments
- Biomedical Ethics and Regulation
- Viral Infections and Vectors
- Assisted Reproductive Technology and Twin Pregnancy
- SARS-CoV-2 and COVID-19 Research
- Male Reproductive Health Studies
- Effects and risks of endocrine disrupting chemicals
- Thallium and Germanium Studies
- Urologic and reproductive health conditions
- COVID-19 and healthcare impacts
- Genital Health and Disease
- Xenotransplantation and immune response
Wake Forest University
2015-2024
Forest Institute
2015-2024
Atrium Health Wake Forest Baptist
2014-2024
Winston-Salem State University
2016-2019
Lund University
2018
Karolinska University Hospital
2018
Karolinska Institutet
2018
California Institute for Regenerative Medicine
2018
Academic Center for Education, Culture and Research
2009-2015
Biotechnology Research Center
2009-2015
Young boys treated with high-dose chemotherapy are often confronted infertility once they reach adulthood. Cryopreserving testicular tissue before and autotransplantation of spermatogonial stem cells at a later stage could theoretically allow for restoration fertility.To establish in vitro propagation human from small biopsies to obtain an adequate number successful transplantation.Study performed April 2007 July 2009 using testis material donated by 6 adult men who underwent orchidectomy as...
Current practices in drug development have led to therapeutic compounds being approved for widespread use humans, only be later withdrawn due unanticipated toxicity. These occurrences are largely the result of erroneous data generated by vivo and vitro preclinical models that do not accurately recapitulate human physiology. Herein, a primary cell- stem cell-derived 3D organoid technology is employed screen panel drugs were recalled from market FDA. The platform comprised multiple tissue...
Existing methods for evaluating the potential gonadotoxicity of environmental agents and pharmaceutical compounds rely heavily on animal studies. The current gold standard in vivo functional assays animals are limited their human predictive capacity. In addition, existing two-dimensional vitro models testicular toxicity do not accurately reflect situation. A more reliable model system is needed to better assess gonadotoxic drugs prior progression into clinical trials. overall goal this study...
Abstract STUDY QUESTION Twenty years after the inception of first fertility preservation programme for pre-pubertal boys, what are current international practices with regard to cryopreservation immature testicular tissue? SUMMARY ANSWER Worldwide, tissue has been cryopreserved from over 3000 boys under age 18 a variety malignant and non-malignant indications; there is variability in related eligibility, clinical assessment, storage, funding. WHAT IS KNOWN ALREADY For male patients receiving...
Expression of angiotensin-converting enzyme 2, receptor severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is high in the testes, therefore SARS-CoV-2 infection and its association with male reproductive health should be investigated disease 2019 patients.
Zika virus (ZIKV) is unique among mosquito-borne flaviviruses in its ability to be sexually transmitted. Persistent ZIKV infection the testes, which are immune privileged organs, long after peripheral clearance suggests involvement of immunosuppressive pathways; however, underlying mechanisms remain undetermined. We recently demonstrated that infects human Sertoli cells (SC), major cell type seminiferous epithelium responsible for maintaining compartment tubules. Recent reports have...
BACKGROUNDGiven the significant drawbacks of using human embryonic stem (hES) cells for regenerative medicine, search alternative sources multipotent is ongoing. Studies in mice have shown that ES-like can be derived from neonatal and adult testis. Here we report derivation
Sertoli cells isolated from the adult mouse and human testis resume proliferation in culture. After 20 days of culture Dulbecco modified Eagle medium/Ham F12 (DMEM/F12) medium containing 5%% fetal calf serum, about 36%% cells, identified by their immunohistochemical staining for cell marker vimentin, incorporated bromodeoxyuridine (BrdU). The renewed was associated with a 70%% decrease expression cycle inhibitor CDKN1B (P27kip1) 2-fold increase levels inducer ID2. In vivo, balance between...
The 2015–2016 Zika virus (ZIKV) epidemic, resulting in >1.5 million cases the Americas, emerged as a global threat to public health due unique findings of sexual and utero transmission...
The hallmark of severe COVID-19 involves systemic cytokine storm and multi-organ injury including testicular inflammation, reduced testosterone, germ cell depletion. ACE2 receptor is also expressed in the resident cells, however, SARS-CoV-2 infection mechanisms are not fully understood. could be initiated by direct virus or exposure to inflammatory mediators viral antigens. We characterized different human 2D 3D culture systems primary Sertoli Leydig mixed seminiferous tubule cells (STC),...