A5057540400- Reproductive Biology and Fertility
- Erythrocyte Function and Pathophysiology
- Tissue Engineering and Regenerative Medicine
- Nanoparticles: synthesis and applications
- Blood properties and coagulation
- Graphene and Nanomaterials Applications
- Mesenchymal stem cell research
- Advanced Nanomaterials in Catalysis
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Prenatal Screening and Diagnostics
- Seaweed-derived Bioactive Compounds
- Skin Protection and Aging
- Microfluidic and Bio-sensing Technologies
- Pelvic floor disorders treatments
- Nanopore and Nanochannel Transport Studies
- Phytochemical and Pharmacological Studies
- Plant responses to water stress
- Erythropoietin and Anemia Treatment
- Organ and Tissue Transplantation Research
- Reproductive System and Pregnancy
- Electrochemical sensors and biosensors
- Endometriosis Research and Treatment
- Assisted Reproductive Technology and Twin Pregnancy
- Hydrogen's biological and therapeutic effects
Kharkiv National Medical University
2021-2025
Institute for Problems of Cryobiology and Cryomedicine
2017-2025
National Academy of Sciences of Ukraine
2017-2025
This work is devoted to the comparison of physical and biological properties synthesized osteoplastic composites with an experimentally determined content (375 μg/g) micro (ZnO
Accumulating evidence suggests that manganese oxide nanoparticles (NPs) show multiple enzyme-mimicking antioxidant activities, which supports their potential in redox-targeting therapeutic strategies for diseases with impaired redox signaling. However, the systemic administration of any NP requires thorough hemocompatibility testing. In this study, we assessed synthesized Mn3O4 NPs, identifying ability to induce spontaneous hemolysis and eryptosis or impair osmotic fragility. Concentrations...
Background. The human umbilical cord is a promising source of biologically active substances with regenerative properties. However, the potential lyophilized cryoextract from for medicine, which could facilitate storage and transportation, remains unexplored. Therefore, it important to study effect such cryoextracts using cellular model. Objective. To evaluate frozen on L929 cell line assess their therapeutic potential. Methods. This was conducted cultures. Cryoextracts were obtained through...
Aim. In this study, blood compatibility of ZnO nanoparticles-polymer nanocomplex (D-PAA/ZnONPs(SO42-)) synthesizedin situinto dextran-graft-polyacrylamide (D-PAA) using zinc sulphate as a precursor was tested hemolysis, osmotic fragility and eryptosis assays.Materials methods. Dose-dependent ability to induce assessed following 24 h incubation at concentrations 0-800 mg l-1analyzing hallmarks (cell shrinkage phosphatidylserine externalization), well reactive oxygen species generation....
Cancer chemotherapy faces two major challenges - high toxicity of active substances and tumor resistance to drugs. Low toxic nanocarriers in combination with anticancer agents can significantly increase the effectiveness therapy. Modern advances nanotechnology make it easy create materials necessary physical chemical properties.Two hybrid nanosystems dextran-polyacrylamide/ zinc oxide nanoparticles (D-PAA/ZnO NPs) were synthesized aqueous solution sulphate NPs (SO42-)) acetate (-OAc)). The...
About 1% of women suffer from premature ovarian failure, which leads to a significant deterioration in the life quality. Most often this condition is caused by performed chemotherapy, autoimmune diseases, surgery on ovaries, uterus, or fallopian tubes. The aim study was compare different approaches tissue and cell therapy restoring sexual function case failure induced chemotherapy. Materials Methods: carried out BALB/c mice with modeled cyclophosphamide busulfan. restoration dynamics...
The aim of the present research was to assess cytotoxicity gold and silver nanoparticles synthesized into dextran-graft-polyacrylamide (D-PAA) polymer nanocarrier, which were used as a basis for further preparation multicomponent nanocomposites revealed high efficacy antitumor therapy. evaluation influence Me-polymer systems on viability metabolic activity fibroblasts eryptosis elucidating mechanisms proeryptotic effects has been done in current research. investigated this study did not...
This study aims to determine the effect of graphene oxide (GO), multilayer carbon tubes (MWCNTs+Fe), magnetite (Fe3O4) and in situ forming brushite (DCPD) nanoparticles (NPs) chitosan (CS) based matrices on cytotoxicity, Pregabalin (PG) release, vivo behaviour mechanically stabilized, conductive nerve conduits (NCs). NPs change composites' hydrophilicity order: DCPD (46.8) ˂ MWCNT (54.21) ~ Fe3O4 (54.44) GO (65.99) water contact angle (47-66 degrees) corresponds conditions cell adhesion. ≤...
The development of new diuretics plant origin is an actual direction. Chimaphila umbellata (L.) a perennial herb with diuretic, astringent, analgesic and other effects; it can treat various conditions such as edema, dropsy, etc. Pipsissewa helps the removal nitrogenous chloride salts from body due to content arbutin glycoside, tannins (up 5 %). aim. Evaluation effect pipsissewa extract on L929 cell culture. Materials methods. Cell line (fibroblasts mouse adipose tissue) was obtained in...
Tumor drug resistance and systemic toxicity are major challenges of modern anticancer therapy. Nanotechnology makes it possible to create new materials with the required properties for
Background. Due to the fact that regenerative medicine has been actively developing, an actual task is study features of functioning biological objects in a patient’s body. Objective. The aim was dynamics human chorionic gonadotropin, alphafetoprotein secretion and biodegradation organism experimental animals cryopreserved cryoextract, suspension cells fragments placenta. Methods. After administering extract, placental we determined content alpha-fetoprotein gonadotropin at days 1-3, 7, 14,...
The methods of cell and tissue therapy, the promising objects those are placental derivatives, affecting namely female reproductive system levels its regulation, have been widely applied in current medicine. mechanisms this interaction still remained unclear. We studied here influence media, conditioned with cryopreserved freshly-isolated cells explants on isolated murine ovarian, uterine cultures that fibroblasts. were evaluated using morphological metabolic tests. Conditioned media shown...
Background: Semi-refined carrageenan (food additive E407a) is a widely used thickener, which has been reported to exert toxic and pro-inflammatory effects. In particular, there accumulating evidence that it induces eryptosis, i.e. programmed cell death of eryptocytes, via ROS-mediated pathways. However, the role Ca2+-dependent mechanisms in E407a-induced eryptosis not elucidated. Material methods: at concentrations 0 mg/ml, 1 5 mg/ml 10 was incubated with blood intact female WAG rats (n=9)...
The development of inexpensive equipment adapted for the study a specific biological object is very important cryobiology. In presented work, we have proposed simple system microscopy utilising open-source platform Arduino. Testing this showed that it had sufficient sensitivity to determine physical processes occurring in cryopreserved sample such as intra- and extracellular water crystallisation salt eutectic. Utilising system, investigated mechanisms cryoprotection cryodamage testis...
<title>Abstract</title> Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity newly synthesized nanostructured carbonate hydroxide (ZnCH) Zn<sub>5</sub>(CO<sub>3</sub>)(OH)<sub>6</sub> microflakes. Materials and methods. Cytotoxicity microflakes was against murine L929 cell line rat mature erythrocytes. MTT neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin...