A5083348030- Pharmacogenetics and Drug Metabolism
- Estrogen and related hormone effects
- Drug Transport and Resistance Mechanisms
- Diabetes Management and Research
- Inflammatory mediators and NSAID effects
- Diabetes and associated disorders
- Bone health and treatments
- Pharmacological Effects and Toxicity Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Acute Lymphoblastic Leukemia research
- Drug-Induced Hepatotoxicity and Protection
- Bone and Joint Diseases
- African Botany and Ecology Studies
- Childhood Cancer Survivors' Quality of Life
- Acute Myeloid Leukemia Research
- Electrolyte and hormonal disorders
- Cancer Treatment and Pharmacology
- Orthopedic Infections and Treatments
- Antioxidant Activity and Oxidative Stress
- BRCA gene mutations in cancer
- Nutrition, Genetics, and Disease
- Cancer Risks and Factors
- Renal Transplantation Outcomes and Treatments
- HIV/AIDS drug development and treatment
- Mycotoxins in Agriculture and Food
Harding University Main Campus
2015-2021
University of Florida
2016
Columbus Oncology and Hematology Associates
2016
Central Hospital of Yaoundé
2014
Indiana University – Purdue University Indianapolis
2010-2011
Indiana University School of Medicine
2010
Zero to Three
2010
St. Jude Children's Research Hospital
2008
Johannes Gutenberg University Mainz
2006
University of Göttingen
2004-2006
Tacrolimus is metabolized predominantly to 13-O-demethyltacrolimus in the liver and intestine by cytochrome P450 3A (CYP3A). Patients with high concentrations of CYP3A5, a CYP3A isoenzyme polymorphically produced these organs, require higher doses tacrolimus, but exact mechanism this association unknown.cDNA-expressed enzymes bank human microsomes known CYP3A4 CYP3A5 content were used investigate contribution metabolism tacrolimus as quantified liquid chromatography-tandem mass...
The hepatic carcinogen aflatoxin B1 (AFB1) is metabolized in the liver by at least four different P450s, all of which exhibit large interindividual differences expression levels. These could affect individual risk hepatocellular carcinoma (HCC). We investigated metabolism AFB1 a panel 13 human microsomal preparations using abundance model, takes into account specific kinetic parameters and levels these P450s. found 12-fold variability production rate carcinogenic metabolite AFB1-8,9-epoxide...
The associations between plasma letrozole concentrations and CYP2A6 CYP3A5 genetic variants were tested in the Exemestane Letrozole Pharmacogenomics (ELPH) trial. ELPH is a multicenter, open-label prospective clinical trial women randomly assigned (n ≈ 250 each arm) to receive 2 years of treatment with either oral (2.5 mg/day) or exemestane (25 mg/day). CYP3A showed effects on metabolism vitro. DNA samples genotyped for genes. Plasma high interpatient variability (>10-fold) associated...
Abstract Glucocorticoid‐induced osteonecrosis is a common and dose‐limiting adverse event. The goal of this study was to establish mouse model glucocorticoid‐induced suitable for testing the effects different treatment strategies on its frequency. Fourteen murine strains were screened using various glucocorticoids, routes administration, diets. Four‐week‐old male BALB/cJ mice treated with oral dexamethasone up 12 weeks either by continuous dosing or discontinuous dosing, without...
Little information is available regarding the metabolic routes of anastrozole and specific enzymes involved. We characterized oxidative conjugation metabolism in vitro vivo.A sensitive LC-MS/MS method was developed to measure its metabolites vivo. Anastrozole using human liver microsomes (HLMs), expressed cytochrome P450s (CYPs) UDP-glucuronosyltransferases (UGTs).Hydroxyanastrozole glucuronide were identified as main conjugated vitro, respectively. Formation hydroxyanastrozole from markedly...
Exemestane is a potent and irreversible steroidal aromatase inhibitor drug used for the treatment of estrogen receptor-positive breast cancer. Our aim was to identify assess contribution specific cytochromes P450 (P450s) responsible exemestane primary in vitro metabolism. With use high-performance liquid chromatography chromatography-tandem mass spectrometry analytical techniques, 17-hydroexemestane (MI) formation 6-hydroxymethylexemestane (MII) were found be predominant metabolic pathways....
Cytochrome P450 3A enzymes (CYP3A) play a major role in the metabolism of steroid hormones, drugs and other chemicals, including many carcinogens. The individually variable CYP3A expression, which remains mostly unexplained, has been suggested to affect clinical phenotypes. We investigated locus five ethnic groups. degree linkage disequilibrium (LD) differed among groups, but most common alleles conserved LD regions were remarkably similar. Non-African haplotypes are few; for example, only...
Glucocorticoids are used universally in the remission induction therapy for acute lymphoblastic leukemia (ALL). One of adverse effects glucocorticoids is hypertension. Our aim was to define frequency and clinical genetic risk factors steroid-induced hypertension.We determined genotypes 203 candidate polymorphisms genes previously linked hypertension or pharmacokinetics pharmacodynamics antileukemic agents. Hypertension defined according guidelines American Academy Pediatrics; patients were...
Exemestane is an aromatase inhibitor drug used for the treatment of hormone-dependent breast cancer. 17-Hydroexemestane, major and biologically active metabolite exemestane in humans, eliminated via glucuronidation by polymorphic UGT2B17 phase II drug-metabolizing enzyme. Previous microsomal studies have shown that gene deletion affects intrinsic hepatic clearances 17-hydroexemestane vitro. In this open-label study we set out to assess effect on pharmacokinetics healthy female volunteers...
Osteonecrosis is one of the most common, serious, toxicities resulting from treatment acute lymphoblastic leukemia. In recent years, pediatric leukemia clinical trials have used discontinuous rather than continuous dosing dexamethasone in an effort to reduce incidence osteonecrosis. However, it not known whether would compromise antileukemic efficacy glucocorticoids. Therefore, we tested against murine models bearing human xenografts (n = 8 patient samples) or BCR-ABL+ Plasma concentrations...
What is known and objective OATP1B1 mediates the transport of a diverse range amphiphilic organic compounds that include bile acids, steroid conjugates hormones. This retrospective pharmacogenetic study was conducted to assess impact c.521T>C single nucleotide polymorphism (SNP) on pharmacokinetics steroidal aromatase inhibitor drug exemestane in healthy volunteers. Methods Exemestane (25 mg) administered orally 14 post-menopausal women. All subjects were sampled for pharmacokinetic (PK)...
Medication non-adherence to treatment regimens can severely impact the mortality of patients afflicted with breast cancer.The purpose this study was identify factors that contribute endocrine therapy in cancer plans.Thirty-two women a diagnosis were surveyed by pharmacists and pharmacy students patient- related (e.g. patient personal beliefs, education level), drug-related drug allergies), socio-economic ability pay for medication) healthcare system poor patient-healthcare provider...