Do peroxiredoxins (PRDXs) control reactive oxygen species (ROS) levels during human sperm capacitation? PRDXs are necessary to the of ROS generated capacitation allowing spermatozoa achieve fertilizing ability. Sperm is an oxidative event that requires low and controlled amounts trigger phosphorylation events. antioxidant enzymes not only act as scavengers but also action in somatic cells. Spermatozoa from infertile men have lower (particularly PRDX6), which thiol-oxidized therefore...

How does l-carnitine (LC) supplementation during vitrification and in vitro maturation (IVM) of germinal vesicle stage (GV)–oocytes improve the developmental competence resultant metaphase II (MII) oocytes? LC both GV–oocytes their subsequent IVM improved nuclear as well meiotic spindle assembly mitochondrial distribution MII oocytes. Vitrification results a lower success rate blastocyst development compared with non-vitrified mouse significantly improves embryonic after IVF. were collected...

Oocyte cryopreservation is important for assisted reproductive technologies (ART). Although of metaphase II (MII) oocytes has been successfully used, MII are vulnerable to the damage inflicted by freezing procedure. Cryopreservation germinal vesicle stage (GV-oocytes) an alternative choice; however, blastocyst development from GV-oocytes limited largely due need in vitro maturation (IVM). Herein, we evaluated effects l-carnitine (LC) supplementation during vitrification and thawing mouse...

Prdx6

Oocytes are exposed to various stressors during in vitro maturation (IVM). Antioxidant supplementation IVM can mitigate oxidative stress. We investigated the effects of supplementing medium with novel flavonoid-enriched antioxidant nanoformulations, namely, EMD-300® and EMP3-H200®, on oocyte analyzed expression stress, apoptosis, pluripotency genes buffalo. Cumulus complexes (COCs) obtained from buffalo ovaries were matured supplemented either or EMP3-H200® at 0.5% 1.0% for 22 h. Following...

Peroxiredoxins (PRDXs) are antioxidant enzymes proven to control the levels of reactive oxygen species (ROS) and avoid oxidative damage in spermatozoon. Previously, we have shown that low amounts PRDXs associated with male infertility PRDX6 is primary defense human spermatozoa, maintaining survival DNA integrity (Gong et al., 2012, Fernandez O'Flaherty, 2018). Oxidative stress can trigger different pathway cascades including truncated apoptosis. It has been reported phosphorylation status...

The cryopreservation of immature oocytes at the germinal vesicle (GV) stage would create an easily accessible, non-seasonal source female gametes for research and reproduction. present study investigated ability ovine vitrified GV using a cryoloop to be subsequently matured, fertilised cultured in vitro blastocyst-stage embryos. Selected cumulus–oocyte complexes obtained from mature ewes time death were randomly divided into vitrified, toxicity control groups. Following vitrification...

Inter-species somatic cell nuclear transfer (iSCNT) embryos usually fail to develop the blastocyst stage and beyond due incomplete reprogramming of donor cell. We evaluated whether using a karyoplast that would require less extensive such as an embryonic blastomere or meiotic spindle from metaphase II oocytes provide additional insight into development iSCNT embryos. Our results showed karyoplasts oocyte origin are no different cells; all embryos, irrespective origin, were arrested during...

Abstract Spermatogenesis consists of a series highly regulated processes that include mitotic proliferation, meiosis and cellular remodeling. Although alterations in gene expression are well known to modulate spermatogenesis, posttranscriptional mechanisms less defined. The ubiquitin proteasome system plays significant role protein turnover may be involved these mechanisms. We previously identified ligase Huwe1 the testis showed it can ubiquitinate histones. Since modulation histones is...

Abstract Melatonin is known to protect sperm against freezing‐inflicted damage in different domestic species. The aim of the study was evaluate effect supplementation semen extender with melatonin on quality and DNA integrity cooled frozen/thawed rabbit spermatozoa. We also investigated whether addition could improve fertility does artificially inseminated semen. Semen samples collected from eight bucks were pooled then diluted INRA‐82 supplemented either (0.5, 1.0 or 1.5 mM) without (0.0...

The development of embryos produced by somatic cell nuclear transfer (SCNT) using vitrified oocytes as cytoplast recipients has been reported in cattle but not sheep. This study investigated the parthenogenetic ovine and thawed at germinal vesicle (GV) stage, matured vitro, then activated two activation protocols. optimal protocol was used to assess when were for SCNT. No blastocysts obtained from CA+CHX/CB (calcium ionophore A23187 + cycloheximide, cytochalasin B); contrast, Sr/CB...

Cryopreservation of oocytes would serve as an alternative to overcome the limited availability dromedary camel and facilitate improvements in IVP techniques this species. Our goal was develop a protocol for vitrification at germinal vesicle (GV) stage using different cryoprotectant combinations: 20% EG DMSO (VS1), 25% plus (VS2) or glycerol (VS3) various cryo-carriers; straws open pulled-straw (OPS) solid surface (SSV); Cryotop. Viable were cultured vitro 30 h. Matured fertilized with...

Oxidative stress has negative impacts on the clinical outcomes of assisted reproduction techniques. The brain-derived neurotrophic factor (BDNF) promotes viability nerve cells and is known to decrease oxidative apoptosis in different cells. aim this study was evaluate effect BDNF treatment human sperm functions that are be essential for fertilisation. Our findings showed spermatozoa with 0.133 nM significantly increased percentages both total (P = 0.001) progressive < 0.01) motile compared...

This study aimed to investigate the effect of L-Carnitine (LC) supplementation during in vitro maturation (IVM) canine oocytes on nuclear maturation, fertilization status, and preimplantation development. Cumulus–oocyte complexes (COCs) collected from ovaries ovariohysterectomized female dogs were matured for 72 h a TCM-199 medium supplemented with (0.1, 0.3, 0.6, 1.0, or 2.0 mg/mL) without (0.0 LC. Matured fertilized frozen–thawed spermatozoa, zygotes cultured SOF 7 days. IVM rates higher...

Oocyte cryopreservation is valuable way of preserving the female germ line. Vitrification immature ovine oocytes decreased levels both maturation promoting factor (MPF) and mitogen-activated protein kinase (MAPK) in metaphase II (MII) after IVM. Our aims were 1) to evaluate effects vitrification GV-oocytes on spindle assembly, MPF/MAP kinases activities, preimplantation development following IVM IVF, 2) elucidate impact caffeine supplementation during quality vitrified/warmed GV-oocytes....

Various techniques such as AI and ET have been reported to improve reproductive efficiency genetic potential in camelids. In vitro fertilization the development of IVP embryos are considered an alternative for improvement this species. This study investigated effects different sperm cell concentrations (1, 2, 3 4 × 106 mL–1), capacitating materials (5 mM caffeine, 10 μg mL–1 heparin, mg theophylline, 1 calcium ionophore A23178 heparin + 5 caffeine), post-slaughter epididymal flushing time...

Adel R. Moawad, Ibrahim M. Ghoneim, Gamal Darwish, Magdy Badr, Diya A. El-Badry and Abou Bakr EL-Wishy. Journal of Animal Reproduction Biotechnology 2020;35:119-41. https://doi.org/10.12750/JARB.35.2.119