A5048267077- Enzyme-mediated dye degradation
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Plant tissue culture and regeneration
- Seed Germination and Physiology
- Microbial Metabolism and Applications
- Anaerobic Digestion and Biogas Production
- Carbohydrate Chemistry and Synthesis
- Botanical Research and Applications
- Biomedical Research and Pathophysiology
- Amino Acid Enzymes and Metabolism
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Dye analysis and toxicity
- Growth and nutrition in plants
- Biochemical and Molecular Research
- Vascular anomalies and interventions
- Molecular Biology Techniques and Applications
- Enzyme Structure and Function
- Biochemical and biochemical processes
- Microbial Natural Products and Biosynthesis
- Electrochemical sensors and biosensors
- Chromium effects and bioremediation
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Nanomaterials in Catalysis
Universidade Estadual Paulista (Unesp)
2013-2024
Núcleo de Pesquisas Aplicadas (Brazil)
2017-2022
MRC Laboratory of Molecular Biology
2016
Universidade de São Paulo
2009
The water buffalo mozzarella cheese is a typical Italian which has been introduced in the thriving Brazilian market last 10 y, with good acceptance by its consumers. Lactic acid bacteria (LAB) play an important role technological and sensory quality of cheese. In this study, aim was to evaluate diversity autochthones viable LAB isolated from under storage. Samples were collected 3 independent trials dairy industry located southeast region Brazil, on 28th day storage, at 4 ºC. characterized...
Laccases (EC 1.10.3.2) are oxidoreductases that belong to the multicopper oxidase subfamily and classified as yellow/white or blue according their absorption spectrum. Yellow laccases more useful for industrial processes since they oxidize nonphenolic compounds in absence of a redox mediator stand out being stable functional under extreme conditions. This study aimed characterize new laccase was predicted be present genome Chitinophaga sp. CB10 - Lac_CB10. Lac_CB10, with molecular mass...
Laccases are multicopper oxidases (MCOs) with a broad application spectrum, particularly in second-generation ethanol biotechnology and the bioremediation of xenobiotics other highly recalcitrant compounds. Synthetic pesticides long environmental persistence, search for their effective has mobilized scientific community. Antibiotics, turn, can pose severe risks emergence multidrug-resistant microorganisms, as frequent use medical veterinary purposes generate constant selective pressure on...
New ß-glucosidases with product (glucose) or ethanol tolerances are greatly desired to make industrial processes more marketable and efficient. Therefore, this report describes the in silico/vitro characterization of Bg10, a metagenomically derived homodimeric ß-glucosidase that exhibited Vmax 10.81 ± 0.43 μM min-1, Kcat 175.1± 6.91 Km 0.49 0.12 mM at neutral pH 37°C when pNP-ß-D-glucopyranoside was used as substrate, enzyme retained greater than 80% activity within respective temperature...
The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. enzyme called MesoAmp exhibited optimum activity at pH 8.5 45 °C strongly activated by Co2+ Mn2+. Under these reaction conditions, the displayed Km kcat values of 0.2364 ± 0.018 mM 712.1 88.12 s-1, respectively. Additionally, showed remarkable stability organic solvents active high concentrations NaCl, suggesting that might be suitable for use biotechnology. is responsible 40% organism's...
Abstract Laccases are multicopper oxidases that act on various phenolic and non-phenolic compounds, enabling numerous applications including xenobiotic bioremediation, biofuel production, drug development, cosmetic they can be used as additives in the textile food industries. This wide range of uses makes these enzymes extremely attractive for novel biotechnology applications. Here, we undertook kinetic characterization LacMeta, a predicted homotrimeric (~ 107,93 kDa) small laccase,...
The filter cake from sugar cane processing is rich in organic matter and nutrients, which favors the proliferation of microorganisms with potential to deconstruct plant biomass. From metagenomic data this material, we assembled a draft genome that was phylogenetically related Thermomonospora curvata DSM 43183, shows functional ecological importance bacterium cake. gram-positive produces cellulases compost, it can survive temperatures 60 ºC. We identified complete set biomass depolymerizing...
O objetivo do presente trabalho foi documentar o processo de germinacao inicial e crescimento in vitro plântulas Cattleya walkeriana sob diferentes meios nutritivos a fim aperfeicoar propagacao dessa especie. Plântulas 90 dias (0,8 ± 0,1 cm comprimento) foram repicadas entre os Vacin & Went, WPM, Knudson-C, Phytamax® ½ MS. Apos (180 depois da semeadura), as caracteristicas biometricas altura planta, numero folhas, raizes, largura maior raiz, comprimento raiz massa materia fresca (parte...
Abstract LacMeta is suitable for the complete degradation and detoxification of malachite green (MG) dye did not form leucomalachite (LCM, analogous compound). The optimal pH temperature parameters were 5.0 50 °C, respectively (MG as substrate). was tolerant up to 10 mmol L-1 EDTA (82%ds) 5% (V/V) acetone (91%ds) methanol (71%ds), while SDS promoted severe inhibition. For ions, a high tolerance cobalt, zinc, manganese, calcium (10 L-1) also observed (> 90%ds). Even under high-salinity...
Os objetivos deste trabalho foram documentar a morfogenese de sementes Cattleya walkeriana por microscopia eletronica varredura e avaliar o crescimento in vitro desta especie grande interesse comercial ecologico, partir do meio cultura MS modificado suplementacao com aditivos orgânicos recomendados para cultivo vitro orquideas
O objetivo do presente trabalho foi o de avaliar os efeitos diferentes doses carvao ativado no crescimento plântulas germinadas in vitro e posterior sobrevivencia em condicoes ex Caularthron bicornutum , especie orquidea nativa Brasil de grande interesse para producao comercial.