A5030426208- Protein Degradation and Inhibitors
- Pancreatic and Hepatic Oncology Research
- Multiple Myeloma Research and Treatments
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Melanoma and MAPK Pathways
- Pancreatic function and diabetes
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Prostate Cancer Treatment and Research
- Polyamine Metabolism and Applications
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Occupational and environmental lung diseases
- Advanced Breast Cancer Therapies
- Quality and Safety in Healthcare
- Chronic Lymphocytic Leukemia Research
- Galectins and Cancer Biology
Northwestern University
2013-2023
The Ohio State University
2017-2023
Banaras Hindu University
2022
Rajendra Institute of Medical Sciences
2021
Ranchi University
2020
Indo-American Center
2018-2019
Robert H. Lurie Comprehensive Cancer Center of Northwestern University
2013-2019
Jesse Brown VA Medical Center
2013-2016
Chang Gung University
2014
Linkou Chang Gung Memorial Hospital
2014
Journal Article Somatic mutations in the PTCH, SMOH, SUFUH and TP53 genes sporadic basal cell carcinomas Get access J. Reifenberger, Reifenberger Departments of Dermatology Neuropathology, Heinrich‐Heine‐University, Moorenstraße 5, D‐40225 Düsseldorf, Germany Julia Reifenberger. E‐mail: reifenbergerj@med.uni‐duesseldorf.de Search for other works by this author on: Oxford Academic Google Scholar M. Wolter, Wolter C. B. Knobbe, Knobbe Köhler, Köhler A. Schönicke, Schönicke Scharwächter,...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is associated with pronounced fibrosis that contributes to chemoresistance, in part, through increased histone acetylation. Because bromodomain (BRD) and extra terminal domain (BET) proteins are “readers” of acetylation marks, we targeted BET PDAC cells grown three-dimensional collagen. We show treatment inhibitors decreases growth (AsPC1, CD18, Panc1) Transfection siRNA against BRD4, which human tumors, also cells. additionally decrease...
Abstract JQ1 and I-BET151 are selective inhibitors of BET bromodomain proteins that have efficacy against a number different cancers. Since the effectiveness targeted therapies is often limited by development resistance, we examined whether it was possible for cancer cells to develop resistance inhibitor JQ1. Here show pancreatic developing demonstrate cross-resistance insensitivity BRD4 downregulation. The resistant maintain expression c-MYC, increase JQ1-target genes FOSL1 HMGA2 evidence...
Cancer stem cells (CSCs) represent a unique sub-population of tumor with the ability to initiate growth and sustain self-renewal. Although CSC biomarkers have been described for various tumors, only few markers identified nasopharyngeal carcinoma (NPC). In this study, we show that CD24+ isolated from human NPC cell lines express genes (Sox2, Oct4, Nanog, Bmi-1, Rex-1), activation Wnt/β-catenin signaling pathway. possess typical characteristics include enhanced proliferation, increased colony...
Pancreatic ductal adenocarcinoma (PDAC) is associated with a pronounced collagen-rich stromal reaction that has been shown to contribute chemo-resistance. We have previously PDAC cells are resistant gemcitabine chemotherapy in the collagen microenvironment because of increased expression chromatin remodeling protein high mobility group A2 (HMGA2). now found human tumors display higher levels histone H3K9 and H3K27 acetylation fibrotic regions. show relative grown on tissue culture plastic,...
The fibrotic reaction, which can account for over 70%-80% of the tumor mass, is a characteristic feature human pancreatic ductal adenocarcinoma (PDAC) tumors. It associated with activation and proliferation stellate cells (PSCs), are key regulators collagen I production fibrosis in vivo. In this report, we show that members bromodomain extraterminal (BET) family proteins expressed primary PSCs isolated from PDAC tumors, BRD4 positively regulating, BRD2 BRD3 negatively expression...
Bromodomain and extraterminal domain (BET) proteins, which are important epigenetic readers, often dysregulated in cancer. While a number of BET inhibitors currently early phase clinical trials, show limited single-agent activity. The purpose this study is to determine if Quercetin, naturally occurring polyphenolic flavonoid found abundant fruits vegetables, can enhance the anti-tumor effects inhibitors. efficacy combination was evaluated vitro xenograft model pancreatic Co-treatment with...
Patients with pancreatic cancer, which is characterized by an extensive collagen-rich fibrotic reaction, often present metastases. A critical step in cancer metastasis epithelial-to-mesenchymal transition (EMT), can be orchestrated the Snail family of transcription factors. To understand role (SNAI1) development, we generated transgenic mice expressing pancreas. Because chronic pancreatitis contribute to Snail-expressing were treated cerulein induce pancreatitis. Although significant tissue...
Eighteen congenital melanocytic naevi (CMN) from 17 patients and 18 dysplastic (DMN) were screened for mutations in the BRAF oncogene (present study) N-ras (in course of two foregoing studies) by single-strand conformational polymorphism (SSCP)/sequencing analysis. demonstrated both types lesion. As a whole, CMN (94.4%) five DMN (27.7%) harboured either or mutations. is frequently found to be mutated human cutaneous melanomas, it may constitute risk factor melanoma formation within DMN.
Human pancreatic ductal adenocarcinoma (PDAC) tumors are associated with dysregulation of mRNA translation. In this report, it is demonstrated that PDAC cells grown in collagen exhibit increased activation the MAPK-interacting protein kinases (MNK) mediate eIF4E phosphorylation. Pharmacologic and genetic targeting MNKs reverse epithelial-mesenchymal transition (EMT), decrease cell migration, reduce expression EMT-regulator ZEB1 without affecting levels. Paradoxically, eIF4E,...
The fibrotic reaction is a characteristic feature of human pancreatic ductal adenocarcinoma (PDAC) tumors. It associated with activation and proliferation stellate cells (PSCs), which are key regulators fibrosis in vivo. While there increasing interest the regulation PD-L1 expression cancer immune cells, other stromal such as PSCs, has not been fully evaluated. Here we show that PSCs vitro express higher mRNA protein levels compared to present PDAC cells. We inhibitors targeting bromodomain...
BET inhibitors (BETi), which target transcription of key oncogenic genes, are currently being evaluated in early-phase clinical trials. However, because BETis show limited single-agent activity, there is increasing interest identifying signaling pathways to enhance the efficacy BETis. Here, we demonstrate increased MNK kinase-dependent eIF4E phosphorylation following treatment with BETis, indicating activation a prosurvival feedback mechanism response PROTACs, promote degradation proteins,...
Pancreatic ductal adenocarcinoma (PDAC) is associated with a pronounced fibro-inflammatory stromal reaction that contributes to tumor progression. A critical step in invasion and metastasis the epithelial-to-mesenchymal transition (EMT), which can be regulated by Snail family of transcription factors. Overexpression (Snai1) mutant Kras(G12D) pancreas transgenic mice, using an elastase (EL) promoter, resulted fibrosis. To identify how modulates inflammation pancreas, we examined effect...
Cancer cells can invade in three-dimensional collagen as single or a cohesive group of that require coordination cell-cell junctions and the actin cytoskeleton. To examine role Gα13, G12 family heterotrimeric G protein, regulating cellular invasion collagen, we established novel method to track cell by membrane type 1 matrix metalloproteinase-expressing cancer cells. We show knockdown Gα13 decreased metalloproteinase-driven proteolytic enhanced E-cadherin-mediated adhesion. E-cadherin...
Combination of BET inhibitor and vinorelbine improves survival in preclinical models brain metastases.
Background: Pancreatic ductal adenocarcinoma (PDAC) is characterized by the presence of dense stroma that enriched in hyaluronan (HA), with increased HA levels associated more aggressive disease. Increased HA-degrading enzymes hyaluronidases (HYALs) are also tumor progression. In this study, we evaluate regulation HYALs PDAC. Methods: Using siRNA and small molecule inhibitors, evaluated using quantitative real-time PCR (qRT-PCR), Western blot analysis, ELISA. The binding BRD2 protein on...
Overactivation of immune responses is a hallmark autoimmune disease pathogenesis. This includes the heightened production inflammatory cytokines such as Tumor Necrosis Factor α (TNFα), and secretion autoantibodies isotypes rheumatoid factor (RF) anticitrullinated protein antibody (ACPA). Fcγ receptors (FcγR) expressed on surface myeloid cells bind Immunoglobulin G (IgG) complexes. Recognition autoantigen-antibody complexes by FcγR induces an phenotype that results in tissue damage further...
Abstract Cells in the pancreas that have undergone acinar-ductal metaplasia (ADM) can transform into premalignant cells eventually become cancerous. Although epithelial-mesenchymal transition regulator Snail (Snai1) cooperate with Kras acinar to enhance ADM development, contribution of Snail-related protein Slug (Snai2) development is not known. Thus, transgenic mice expressing and were generated. Surprisingly, attenuated Kras-induced ERK1/2 phosphorylation proliferation. Co-expression also...
event.Documents or records provide evidence that the products are manufactured according to pre-developed processes and predefined specifications.Good Documentation Practice (GDP GDocP), a term used in pharmaceutical industry, is essential for integrity of data collection reporting supporting development, registrations, commercialization, life-cycle management products. 1Adhering GDPs assures preventing errors within manufacturing environment during analysis which could otherwise impact...