A5012256414- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Ion Channels and Receptors
- Monoclonal and Polyclonal Antibodies Research
- Urinary Tract Infections Management
- Urinary Bladder and Prostate Research
- Plant Virus Research Studies
- Peripheral Neuropathies and Disorders
- Tuberculosis Research and Epidemiology
- Herpesvirus Infections and Treatments
- Neuroendocrine regulation and behavior
- Myasthenia Gravis and Thymoma
- Tissue Engineering and Regenerative Medicine
- Ion Transport and Channel Regulation
- Influenza Virus Research Studies
- Powdery Mildew Fungal Diseases
- Cutaneous lymphoproliferative disorders research
- Bacterial Identification and Susceptibility Testing
- Systemic Sclerosis and Related Diseases
- Otolaryngology and Infectious Diseases
- Electrolyte and hormonal disorders
- Legionella and Acanthamoeba research
- Antimicrobial Resistance in Staphylococcus
University of Pittsburgh
2015-2024
Institut National de la Recherche Scientifique
2002
Mycobacterium abscessus infections in cystic fibrosis patients are challenging to treat due widespread antibiotic resistance. The therapeutic use of lytic bacteriophages presents a new potential strategy, but the great variation among clinical M. isolates demands determination phage susceptibility prior therapy.
Mycobacterium abscessus is an important emerging pathogen that challenging to treat with current antibiotic regimens. There substantial genomic variation in M. clinical isolates, but little known about how this influences pathogenicity and vivo growth.
Abstract Advances in genome sequencing have produced hundreds of thousands bacterial sequences, many which integrated prophages derived from temperate bacteriophages. These play key roles by influencing metabolism, pathogenicity, antibiotic resistance, and defense against viral attack. However, they vary considerably even among related strains, are challenging to identify computationally extract precisely for comparative genomic analyses. Here, we describe DEPhT, a multimodal tool prophage...
Abstract The diversity and mosaic architecture of phage genomes present challenges for whole-genome phylogenies comparative genomics. There are no universally conserved core genes, ∼70% genes unknown function, replete with small (<500 bp) open reading frames. Assembling sequence-related into “phamilies” (“phams”) based on amino acid sequence similarity simplifies genomics facilitates representations genome mosaicism. With the rapid substantial increase in numbers sequenced genomes,...
The basal, intermediate, and superficial cell layers of the urothelium undergo rapid complete recovery following acute injury; however, effects chronic injury on urothelial regeneration have not been well defined. To address this discrepancy, we employed a mouse model to explore changes in response spinal cord (SCI), condition characterized by life-long bladder dysfunction. One day post SCI there was focal loss umbrella cells, which are large cells that populate layer normally express...
Bacteriophage genomes are pervasively mosaic, presenting challenges to describing phage relatedness. Here, we describe PhamClust, a bioinformatic approach for genome comparisons that uses new metric of proteomic equivalence quotient comparative genomics. PhamClust reliably assorts into groups or clusters related phages and can subdivide subclusters. is computationally efficient readily process thousands genomes. It also useful analytic tool exploring the different types inter-genome...
Mycobacterium species include several human pathogens and mycobacteriophages show potential for therapeutic use to control infections. However, phage infection profiles vary greatly among abscessus clinical isolates therapies must be personalized individual patients. susceptibility is likely determined primarily by accessory parts of bacterial genomes, we have identified the prophage phage-related genomic regions across sequenced strains. The prophages are numerous diverse, especially in M....
In April 2024, ten cats died in a rural South Dakota (SD) residence, showing respiratory and neurological symptoms. Necropsy laboratory testing of two confirmed H5N1 clade 2.3.4.4b infection. The viral genome sequences are closely related to recent SD cattle sequences. Cat genomes had unique mutations, including T143A haemagglutinin, known affect infectivity immune evasion, novel mutations PA protein (F314L, L342Q) that may polymerase activity virulence, suggesting potential virus...
A multiparametric flow cytometry antimicrobial susceptibility test was developed and its performance evaluated on clinical urine isolates samples in comparison with standard methods. Alterations cytoplasmic membrane integrity were monitored by propidium iodide, the anionic probe bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) used to measure changes potential. Microbial size cellular content analysed light scattering. Twelve antibiotics tested 6 ATCC control strains, 22 19...
The mechanisms underlying detection and transmission of sensory signals arising from visceral organs, such as the urethra, are poorly understood. Recently, specialized ACh-expressing cells embedded in urethral epithelium have been proposed chemosensory sentinels for bacterial infection. Here, we examined morphology potential role signalling a different class that express serotonin (5-HT), termed paraneurones.
Bacteriophages (phages) are incredibly abundant and genetically diverse. The volume of phage genomics data is rapidly increasing, driven in part by the SEA-PHAGES program, which isolates, sequences manually annotates hundreds genomes each year. With an ever-expanding dataset, there many opportunities for generating new biological insights through comparative genomic bioinformatic analyses. As a result, growing need to be able store, update, explore analyze data. package pdm_utils provides...
Mycobacterium abscessus infections are relatively common in patients with cystic fibrosis and clinically challenging, frequent intrinsic resistance to antibiotics. Therapeutic treatment bacteriophages offers some promise but faces many challenges including substantial variation phage susceptibilities among clinical isolates, the need personalize therapies for individual patients. Many strains not susceptible any phages or efficiently killed by lytic phages, all smooth colony morphotype...
Mycobacteriophage phiT45-1 is a newly isolated bacteriophage spontaneously released from Mycobacterium abscessus strain Taiwan-45 that lytically infects M. BWH-C; also ATCC 1997 but not smegmatis . Phage has 43,407-bp genome and carries polymorphic toxin-immunity cassette associated with type VII secretion systems.
You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology Pharmacology II1 Apr 2015MP21-16 ROLE OF TRPM4 ON MORPHOLOGICAL AND FUNCTIONAL CHANGES IN THE NEUROGENIC BLADDER F. Aura Kullmann, Jonathan Beckel, Hiroshi Nakagomi, Amanda Wolf-Johnston, Christian Gauthier, Anthony Kanai, Lori Birder KullmannF. Kullmann More articles by this author , BeckelJonathan Beckel NakagomiHiroshi Nakagomi Wolf-JohnstonAmanda Wolf-Johnston GauthierChristian Gauthier KanaiAnthony Kanai...
Spinal cord injury (SCI) profoundly alters bladder function. Chronically, it induces urothelial hyperplasia, smooth muscle hypertrophy and detrusor hyperactivity, all of which prevent the from properly storing releasing urine. Transient receptor potential melastatin 4 (TRPM4) is a non‐selective cation channel activated by intracellular Ca 2+ modulated ATP. This has been implicated in various pathologies including SCI, inflammation, axonal degeneration cardiac myocyte hypertrophy. We...