A5080678441- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Plant Molecular Biology Research
- Irrigation Practices and Water Management
- Polymer-Based Agricultural Enhancements
- Legume Nitrogen Fixing Symbiosis
- Erythrocyte Function and Pathophysiology
- Cellular Mechanics and Interactions
- Autophagy in Disease and Therapy
- Plant and Biological Electrophysiology Studies
- Plant and animal studies
- Plant Parasitism and Resistance
- 3D Printing in Biomedical Research
- Viral Infectious Diseases and Gene Expression in Insects
- Redox biology and oxidative stress
- Leaf Properties and Growth Measurement
- Plant-Microbe Interactions and Immunity
- Research in Cotton Cultivation
- Remote Sensing in Agriculture
- Electrowetting and Microfluidic Technologies
- Single-cell and spatial transcriptomics
- Plant Reproductive Biology
- Botanical Research and Chemistry
- Mycorrhizal Fungi and Plant Interactions
- Microfluidic and Capillary Electrophoresis Applications
Heidelberg University
2020-2024
Institut de Biologie Intégrative de la Cellule
2017-2022
Heinrich Heine University Düsseldorf
2021
Cluster of Excellence on Plant Sciences
2021
Université Paris-Saclay
2016-2020
Centre National de la Recherche Scientifique
2019-2020
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2020
CEA Paris-Saclay
2016-2019
Université Paris-Sud
2016-2019
In the plant meristem, tissue-wide maturation gradients are coordinated with specialized cell networks to establish various developmental phases required for indeterminate growth. Here, we used single-cell transcriptomics reconstruct protophloem trajectory from birth of progenitors terminal differentiation in
Root hairs are involved in water and nutrient uptake, thereby plant autotrophy. In legumes, they also play a crucial role establishment of rhizobial symbiosis. To obtain holistic view Medicago truncatula genes expressed root their regulation during the first hours engagement symbiotic interaction, high throughput RNA sequencing on isolated from roots challenged or not with lipochitooligosaccharides Nod factors (NF) for 4 h 20 was carried out. This provided repertoire displaying expression...
Thioredoxins (TRXs) are key players within the complex response network of plants to environmental constraints. Here, physiological implication plastidial y-type TRXs in Arabidopsis drought tolerance was examined. We previously showed that y1 and y2 have antioxidant functions, here, corresponding single double mutant were studied context water deprivation. TRX y reduced stress comparison with wild-type (WT) correlated an increase their global protein oxidation levels. Furthermore, at level...
The acquisition of quantitative information on plant development across a range temporal and spatial scales is essential to understand the mechanisms growth. Recent years have shown emergence imaging methodologies that enable capture analysis growth, from dynamics molecules within cells measurement morphometricand physiological traits in field-grown plants. In some instances, these methods can be parallelized multiple samples increase throughput. When high throughput combined with...
Abstract Arabidopsis root is a classic model system in plant cell and molecular biology. The sensitivity of roots to local environmental perturbation challenges data reproducibility incentivizes further optimization imaging phenotyping tools. Here we present RoPod, an easy-to-use toolkit for low-stress live time-lapse roots. RoPod comprises dedicated protocol cultivation customizable 3D-printed vessel with integrated microscopy-grade glass that serves simultaneously as growth chamber....
Significance Mechanosensitive (MS) channels embedded in the membrane provide an efficient system to rapidly convert mechanical tension into a biological signal. Here we show that plasma-membrane mechanosensitive channel MscS-LIKE 10 (MSL10) responds pulsed stretching with rapid activation and relaxation kinetics allowing fast adjustment of its activity fluctuations tension. Under sinusoidal stimulation, found MSL10 amplifies oscillatory signals near 1 Hz which corresponds frequencies plant...
Abstract Strigolactones (SLs) are a class of plant hormones that mediate biotic interactions and modulate developmental programs in response to endogenous exogenous stimuli. However, comprehensive view on the spatio-temporal pattern SL signaling has not been established, tools for systematic planta analysis do exist. Here, we present Strigo-D2, genetically encoded ratiometric sensor enables examination distribution at cellular resolution is capable rapid altered levels intact Arabidopsis...
The root is an important organ for water and nutrient uptake, soil anchorage. It equipped with hairs (RHs) which are elongated structures increasing the exchange surface soil. RHs also studied as a model plant cellular development, they represent single cell specific highly regulated polarized elongation. For these reasons, it useful to be able accurately quantify RH length employing standardized procedures. Methods commonly employed rely on manual steps therefore time consuming prone...
Abstract Arabidopsis root is a classic model system in plant cell and molecular biology. The sensitivity of roots to local environmental perturbation challenges data reproducibility incentivizes further optimization imaging phenotyping tools. Here we present RoPod, an easy-to-use toolkit for low-stress live time-lapse roots. RoPod comprises dedicated protocol cultivation customizable 3D-printed vessel with integrated microscopy-grade glass that serves simultaneously as growth chamber....
Abstract Throughout their life, plants are submitted to recurrent cyclic mechanical loading due wind. The resulting passive oscillation movements of stem and foliage is an important phenomenon for biological ecological issues such as photosynthesis optimization 1–3 thermal exchange 4 . induced motions at plant scale well described analyzed, with oscillations typically 1 3 Hz in trees 5–10 However, the cellular perception transduction recurring signals remains open question. Multimeric...
Abstract Strigolactones (SLs) are a class of plant hormones modulating developmental programs in response to endogenous and exogenous stimuli mediating biotic interactions. However, comprehensive view on the spatio-temporal pattern SL signaling has not been established tools for systematic planta analysis do exist. Here, we present Strigo-D2, genetically encoded ratiometric sensor, allowing examination distribution with cellular resolution its rapid altered levels intact plants. By...