A5051329992- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- COVID-19 diagnosis using AI
- Respiratory viral infections research
- Inhalation and Respiratory Drug Delivery
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Mobile Health and mHealth Applications
- Analytical Chemistry and Sensors
- Nosocomial Infections in ICU
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
California Institute of Technology
2020-2023
Massachusetts Institute of Technology
2016-2018
Abstract There is a need for large-scale, longitudinal studies to determine the mechanisms by which gut microbiome and its interactions with host affect human health disease. Current methods profiling typically utilize next-generation sequencing applications that are expensive, slow, complex. Here, we present synthetic biology platform affordable, on-demand, simple analysis of samples using RNA toehold switch sensors in paper-based, cell-free reactions. We demonstrate species-specific...
Early detection of SARS-CoV-2 infection is critical to reduce asymptomatic and presymptomatic transmission, curb the spread variants, maximize treatment efficacy. Low-analytical-sensitivity nasal-swab testing commonly used for surveillance symptomatic testing, but ability these tests detect earliest stages has not been established. In this study, conducted between September 2020 June 2021 in greater Los Angeles County, California, area, initially SARS-CoV-2-negative household contacts...
SARS-CoV-2 viral-load measurements from a single-specimen type are used to establish diagnostic strategies, interpret clinical-trial results for vaccines and therapeutics, model viral transmission, understand virus-host interactions. However, implicitly assumed be representative of other specimen types. We quantified timecourses individuals who began daily self-sampling saliva, anterior-nares (nasal), oropharyngeal (throat) swabs before or at the incidence infection with Omicron variant....
Abstract Early detection of SARS-CoV-2 infection is critical to reduce asymptomatic and pre-symptomatic transmission, curb the spread variants by travelers, maximize treatment efficacy. Low-sensitivity nasal-swab testing (antigen some nucleic-acid-amplification tests) commonly used for surveillance symptomatic testing, but ability low-sensitivity tests detect earliest stages has not been established. In this case-ascertained study, initially-SARS-CoV-2-negative household contacts individuals...
Postoperative infection and thromboembolism represent significant sources of morbidity mortality but cannot be easily tracked after hospital discharge. Therefore, a molecular test that could performed at home would significantly impact disease management. Our lab has previously developed intravenously delivered 'synthetic biomarkers' respond to dysregulated proteases produce urinary signal. These assays, however, have been limited chronic diseases or acute initiated the time diagnostic...
Abstract Transmission of SARS-CoV-2 in community settings often occurs before symptom onset, therefore testing strategies that can reliably detect people the early phase infection are urgently needed. Early detection is especially critical to protect vulnerable populations who require frequent interactions with caretakers. Rapid COVID-19 tests have been proposed as an attractive strategy for surveillance, however a limitation most rapid their low sensitivity. Low-sensitivity comparable high...
In a recent household transmission study of SARS-CoV-2, we found extreme differences in SARS-CoV-2 viral loads among paired saliva, anterior nares swab (ANS), and oropharyngeal specimens collected from the same time point. We hypothesized these may hinder low-analytical-sensitivity assays (including antigen rapid diagnostic tests [Ag-RDTs]) by using single specimen type (e.g., ANS) reliably detecting infected infectious individuals. evaluated daily at-home ANS Ag-RDTs (Quidel QuickVue)...
Our findings suggest that collecting saliva and nasal swab specimens in the morning immediately after waking yields higher SARS-CoV-2 viral loads than collection later day. The from specimen are predicted to significantly improve detection of symptomatic individuals, particularly when using moderate- low-analytical-sensitivity COVID-19 diagnostic tests, such as rapid antigen tests.
Respiratory tract infections represent a significant public health risk, and timely accurate detection of bacterial facilitates rapid therapeutic intervention. Furthermore, monitoring the progression after intervention enables 'course correction' in cases where initial treatments are ineffective, avoiding unnecessary drug dosing that can contribute to antibiotic resistance. However, current diagnostic techniques rely on non-specific or slow readouts, such as radiographic imaging sputum...
During a household-transmission field study using COVID-19 antigen rapid diagnostic tests (Ag-RDT), common test strip lot was identified among 3 participants with false-positive results. In blinded laboratory evaluation, this exhibited significantly higher rate than other lots. Because positive Ag-RDT result often prompts action, reducing lot-specific false positives can maintain confidence and actionability of true-positive
ABSTRACT Background In a recent household-transmission study of SARS-CoV-2, we found extreme differences in SARS-CoV-2 viral loads among paired saliva, anterior-nares swab (ANS) and oropharyngeal specimens collected from the same timepoint. We hypothesized these may hinder low-analytical-sensitivity assays (including antigen rapid diagnostic tests, Ag-RDTs) using single specimen type (e.g., ANS) reliably detecting infected infectious individuals. Methods evaluated daily at-home ANS Ag-RDT...
ABSTRACT Background The analytical sensitivities of SARS-CoV-2 diagnostic tests span 6 orders magnitude. Optimizing sample-collection methods to achieve the most reliable detection for a given sensitivity would increase effectiveness testing and minimize COVID-19 outbreaks. Methods From September 2020 April 2021 we performed household-transmission study in which participants self-collected samples every morning evening throughout acute infection. Seventy mildly symptomatic collected saliva...
ABSTRACT SARS-CoV-2 viral load measurements from a single specimen type are used to establish diagnostic strategies, interpret clinical-trial results for vaccines and therapeutics, model transmission, understand virus-host interactions. However, implicitly assumed be representative of other types. We quantified viral-load timecourses individuals who began daily self-sampling saliva, anterior nares (nasal), oropharyngeal (throat) swabs before or at the incidence infection with Omicron...