A5063578838- Epigenetics and DNA Methylation
- Biotin and Related Studies
- Bladder and Urothelial Cancer Treatments
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Urinary and Genital Oncology Studies
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- RNA modifications and cancer
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Birth, Development, and Health
- Kruppel-like factors research
- Histone Deacetylase Inhibitors Research
- Multiple Myeloma Research and Treatments
- Congenital heart defects research
- interferon and immune responses
- Genetics and Neurodevelopmental Disorders
- melanin and skin pigmentation
- Cancer-related molecular mechanisms research
- Protein Degradation and Inhibitors
- Respiratory viral infections research
- Cancer Genomics and Diagnostics
- Nutrition, Genetics, and Disease
Murphy Oil Corporation (United States)
2016
University of Maryland, Baltimore
2010-2012
University of Nebraska–Lincoln
2005-2010
Nutrition Sciences (Belgium)
2007
Abstract Polycomb group (PcG) protein-dependent histone methylation and ubiquitination drives chromatin compaction leading to reduced tumor suppressor expression increased cancer cell survival. Green tea polyphenols S -adenosylhomocysteine (AdoHcy) hydrolase inhibitors are important candidate chemopreventive agents. Previous studies indicate that (-)-epigallocatechin-3-gallate (EGCG), a potent green polyphenol, suppresses PcG protein level skin Inhibition of AdoHcy with 3-deazaneplanocin A...
Abstract Claims surrounding exceptional longevity are sometimes disputed or dismissed for lack of credible evidence. Here, we present three DNA methylation-based age estimators (epigenetic clocks) verifying claims centenarians. The centenarian clocks were developed based on n = 7039 blood and saliva samples from individuals older than 40, including 184 centenarians, 122 semi-supercentenarians (aged 105 +), 25 supercentenarians 110 +). oldest individual was 115 years old. Our most accurate...
SARS-CoV-2 viral-load measurements from a single-specimen type are used to establish diagnostic strategies, interpret clinical-trial results for vaccines and therapeutics, model viral transmission, understand virus-host interactions. However, implicitly assumed be representative of other specimen types. We quantified timecourses individuals who began daily self-sampling saliva, anterior-nares (nasal), oropharyngeal (throat) swabs before or at the incidence infection with Omicron variant....
We explored the accuracy of a urine-based epigenetic test for detecting upper tract urothelial carcinoma.Under an Institutional Review Board-approved protocol, urine samples were prospectively collected from primary carcinoma patients before radical nephroureterectomy, ureterectomy, or ureteroscopy between December 2019 and March 2022. Samples analyzed with Bladder CARE, that measures methylation levels 3 cancer biomarkers (TRNA-Cys, SIM2, NKX1-1) 2 internal control loci using...
The polycomb group (PcG) genes encode a family of proteins that methylate and ubiquitinate histones to close chromatin suppress gene expression. PcG are present at elevated levels in cancer cells, this is associated with reduced tumor suppressor protein level enhanced cell survival. Agents reduce regarded as potentially cancer-preventative agents. Sulforaphane (SFN) biologically important isothiocyanate found cruciferous vegetables an candidate chemopreventive agent. However, the impact SFN...
Abstract Background Bladder cancer (BC) is the 5th most common in USA. Non-muscle invasive bladder represents about 70% of all cases and has generally a favorable outcome. However, recurrence rates as high 60 to progression 10 20% necessitate intensive surveillance with cystoscopy. The invasiveness cost cystoscopy poses significant burden on BC patients well healthcare system. In this study we test feasibility simple, sensitive, non-invasive detection using CARE urine samples. Results Urine...
PKCδ increases keratinocyte differentiation and suppresses proliferation survival. However, the mechanism of suppression is not well understood. The present studies show that overexpression p21(Cip1) mRNA protein level promoter activity treatment with dominant-negative PKCδ, PKCδ-siRNA, or rottlerin inhibits activation. Analysis upstream regulatory region reveals three DNA segments mediate PKCδ-dependent response element most proximal to transcription start site encodes six GC-rich elements....
Epigenetic clocks are based on age-associated changes in DNA methylation of CpG-sites, which can accurately measure chronological age different species.Recently, several studies have indicated that the difference between and epigenetic age, defined as acceleration, could reflect biological indicating functional decline diseases.In humans, an clock associated Alzheimer's disease (AD) pathology with acceleration age.In this study, we developed validated two mouse brain regionspecific from...
Sulforaphane (SFN) is an important cancer preventive agent derived from cruciferous vegetables. We show that SFN treatment suppresses normal human keratinocyte proliferation via a mechanism involves increased expression of p21(Cip1). produces concentration-dependent increase in p21(Cip1) promoter activity stabilization the p53 protein leading to binding response elements. The proximal GC-rich Sp1 factor elements are also required, as SFN-dependent lost when these sites mutated. increases...
ChIP-seq is the primary technique used to investigate genome-wide protein-DNA interactions. As part of this procedure, immunoprecipitated DNA must undergo "library preparation" enable subsequent high-throughput sequencing. To facilitate analysis biopsy samples and rare cell populations, there has been a recent proliferation methods allowing sequencing library preparation from low-input amounts. However, little information exists on relative merits, performance, comparability biases inherent...
Inflammaging is a theory of ageing which purports that low-level chronic inflammation leads to cellular dysfunction and premature surrounding tissue. Skin susceptible inflammaging because it the first line defence from environment, particularly solar radiation. To better understand impact photoexposure on epidermal biology, we performed system biology-based analysis photoexposed face arm, photoprotected buttock sites, women between ages 20s 70s. Biopsies were analysed by histology,...
In a recent household transmission study of SARS-CoV-2, we found extreme differences in SARS-CoV-2 viral loads among paired saliva, anterior nares swab (ANS), and oropharyngeal specimens collected from the same time point. We hypothesized these may hinder low-analytical-sensitivity assays (including antigen rapid diagnostic tests [Ag-RDTs]) by using single specimen type (e.g., ANS) reliably detecting infected infectious individuals. evaluated daily at-home ANS Ag-RDTs (Quidel QuickVue)...
SUMMARY There are numerous hallmarks of aging in mammals, but no unifying cause has been identified. In budding yeast, is associated with a loss epigenetic information that occurs response to genome instability, particularly DNA double-strand breaks (DSBs). Mammals also undergo predictable changes age, including alterations methylation patterns serve as “age” clocks, what drives these not known. Using transgenic mouse system called “ICE” (for inducible c hanges the e pigenome), we show...
Our previous study shows that inhibiting activator protein one (AP1) transcription factor function in murine epidermis, using dominant-negative c-jun (TAM67), increases cell proliferation and delays differentiation. To understand the mechanism of action, we compare TAM67 impact mouse epidermis cultured normal human keratinocytes. We show localizes nucleus where it forms homodimers competitively interact with AP1 DNA binding sites to reduce endogenous jun fos binding. Involucrin is a marker...