A5049539246- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Cancer Genomics and Diagnostics
- COVID-19 Impact on Reproduction
- Plant responses to water stress
- Single-cell and spatial transcriptomics
- Plant-Microbe Interactions and Immunity
- Reproductive System and Pregnancy
- COVID-19 and healthcare impacts
- Social Sciences and Governance
- Aging, Elder Care, and Social Issues
- Plant tissue culture and regeneration
- Cancer-related molecular mechanisms research
- Geological and Geophysical Studies Worldwide
- Molecular Biology Techniques and Applications
- Agriculture, Land Use, Rural Development
- Agriculture Sustainability and Environmental Impact
- Sustainable Agricultural Systems Analysis
- Gene expression and cancer classification
- Toxin Mechanisms and Immunotoxins
- Human Rights and Development
- HIV Research and Treatment
- Advanced Electron Microscopy Techniques and Applications
Institut du Développement Durable et des Relations Internationales
2024
Institut d'Etudes Politiques de Paris
2024
Takara (United States)
2016-2022
University of California, Berkeley
2009-2014
Plant Gene Expression Center
2008-2013
United States Department of Agriculture
2009-2013
Agricultural Research Service
2013
McGill University Health Centre
2010
Université Laval
2002-2007
Digital Research Alliance of Canada
2005
KNOTTED1 (KN1)-like homeobox (KNOX) transcription factors function in plant meristems, self-renewing structures consisting of stem cells and their immediate daughters. We defined the KN1 cistrome maize inflorescences found that binds to several thousand loci, including 643 genes are modulated one or multiple tissues. These direct targets strongly enriched for (including other genes) participating hormonal pathways, most significantly auxin, demonstrating plays a key role orchestrating upper...
Abstract KNOTTED1 (KN1)-like homeobox (KNOX) transcription factors are involved in the establishment and maintenance of plant meristems; however, few direct targets KNOX proteins have been recognized. Using a combination double mutant analysis biochemistry, we found that maize (Zea mays), KN1 negatively modulates accumulation gibberellin (GA) through control ga2ox1, which codes for an enzyme inactivates GA. The ga2ox1 mRNA level is elevated immature leaves dominant mutants downregulated...
Protein coding genes constitute approximately 1% of the human genome but harbor 85% mutations with large effects on disease-related traits. Therefore, efficient strategies for selectively sequencing complete regions (i.e., "whole exome") have potential to contribute our understanding diseases. We used a method whole-exome coupling Agilent capture Illumina DNA-sequencing platform, and investigated two unrelated fetuses from nonconsanguineous families Fowler Syndrome (FS), stereotyped...
Maize leaves have distinct tissues that serve specific purposes. The blade tilts back to photosynthesize and the sheath wraps around stem provide structural support protect young leaves. At junction between are ligule auricles, both of which absent in recessive liguleless1 (lg1) mutant. Using an antibody against LG1, we reveal LG1 accumulation at site formation axil developing tassel branches. dominant mutant Wavy auricle blade1 (Wab1-R) produces ectopic tissue increases domain accumulation....
Single cell RNA sequencing methods have been increasingly used to understand cellular heterogeneity. Nevertheless, most of these suffer from one or more limitations, such as focusing only on polyadenylated RNA, the 3' end transcript, an exuberant fraction reads mapping ribosomal and unstranded nature data. Here, we developed a novel single strand-specific total library preparation method addressing all aforementioned shortcomings. Our was validated microfluidics system using three different...
Abstract Maize (Zea mays) leaves provide a useful system to study how proximal/distal patterning is established because of the distinct tissues found in distal blade and proximal sheath. Several mutants disrupt this pattern, including dominant knotted1-like homeobox (knox) mutants. knox genes encode homeodomain proteins TALE superclass transcription factors. Class I are expressed meristem down-regulated as initiate. Gain-of-function phenotypes result from misexpression leaves. We identified...
Bax inhibitor‐1 (BI‐1) protein is proposed to be a conserved programmed cell death suppressor. In this report, we investigate the anti‐apoptotic function of plant BI‐1 by antisense (AS) down regulation NtBI‐1 in Nicotiana tabacum cv. BY‐2 cells. We observed that AS lines were more susceptible autophagy, internucleosomal DNA fragmentation and than control cells when subjected sucrose starvation hypo‐osmotic shock, agreement with role as inhibitor.
ChIP-seq is the primary technique used to investigate genome-wide protein-DNA interactions. As part of this procedure, immunoprecipitated DNA must undergo "library preparation" enable subsequent high-throughput sequencing. To facilitate analysis biopsy samples and rare cell populations, there has been a recent proliferation methods allowing sequencing library preparation from low-input amounts. However, little information exists on relative merits, performance, comparability biases inherent...
Abstract The knotted1 (kn1) homeobox (knox) gene family was first identified through gain-of-function dominant mutants in maize (Zea mays). Class I knox members are expressed meristems but excluded from leaves. In maize, a loss-of-function phenotype has only been characterized for kn1. To assess the function of another member, we mutation rough sheath1 (rs1). rs1-mum1 no alone exacerbates several aspects kn1 phenotype. permissive backgrounds which grow to maturity, loss single copy rs1...
Abstract The spread of SARS-CoV-2 has led to a devastating pandemic, with infections resulting in range symptoms collectively known as COVID-19. full repertoire human tissues and organs susceptible infection is an area active investigation, some studies have implicated the reproductive system. effects COVID-19 on reproduction remain poorly understood, particularly impact early embryogenesis establishment pregnancy are not known. In this work, we explore susceptibility embryos infection. By...
Leaf development presents a tremendous resource for tackling the question of patterning in biology. Leaves can be simple or highly dissected. They may have elaborated parts such as tendrils pea leaf rolled blade carnivorous pitcher plant. Despite variation size, shape, and function, all leaves initiate same manner: from flanks meristem. The maize is useful analysis due to wealth mutants distinct tissues along proximal distal axis. distal, sheath proximal, ligule forms at blade/sheath...
Homeodomain proteins are well-characterized developmental regulators that control expression of target genes through sequence-specific DNA binding. The homeodomain forms a trihelical structure, with the third helix conferring specific interactions major groove. A class plant proteins, called KNOX [KNOTTED1 (KN1)-like homeobox], also has ability to signal between cells by directly trafficking intercellular channels plasmodesmata. Trafficking is mediated contained within homeodomain. Movement...
• Premise of the study: How a leaf acquires its shape is major and largely unresolved question in plant biology. This problem particularly complex case compound leaves, where blade subdivided into leaflets. In many eudicots with class I KNOTTED1‐LIKE HOMEOBOX ( KNOX ) genes are upregulated primordium promote leaflet initiation, while restricted to shoot apical meristem simple‐leaved plants. monocots, however, little known about extent contribution development, we aimed address this issue...
Next-generation sequencing is empowering a deeper understanding of biology by enabling RNA expression analysis over the entire transcriptome with high sensitivity and wide dynamic range. One powerful application within this field stranded (RNA-seq), which necessary to distinguish overlapping genes conduct comprehensive annotation quantification long non-coding RNAs. Commonly used methods for generating strand-specific RNA-seq libraries are often complicated protocols that require several...
Chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-seq) has become the gold standard for mapping of transcription factors and histone modifications throughout genome. However, ChIP experiments involving few cells or targeting low-abundance factors, small amount DNA recovered makes ligation adapters very challenging. In this unit, we describe a ChIP-seq workflow that can be applied to cell numbers, including robust single-tube ligation-free method preparation...
Abstract The spread of SARS-CoV-2 has led to a devastating pandemic, with infections resulting in range symptoms collectively known as COVID-19. full repertoire human tissues and organs susceptible infection is an area active investigation, some studies have implicated the reproductive system. effects COVID-19 on reproduction remain poorly understood, particularly impact early embryogenesis establishment pregnancy are not known. In this work, we explore susceptibility embryos infection. We...
Coalition theory has developed outside the field of international relations in wake game theory, laboratory experiments, and study government coalitions. Four parameters can be used to predict formation development coalitions : benefits sought by players, resources at their disposal, non-utilitarian linkages between them, decision threshold reached. In relations, many studies have dealt with coalitions, alignments, interstate alliances. They grouped into three schools thought. First, there...