A5064727938- Cell Image Analysis Techniques
- Medicinal Plants and Neuroprotection
- Peroxisome Proliferator-Activated Receptors
- Epigenetics and DNA Methylation
- Eicosanoids and Hypertension Pharmacology
- Cardiovascular Disease and Adiposity
- Circadian rhythm and melatonin
- Biomarkers in Disease Mechanisms
- Effects of Radiation Exposure
- Mitochondrial Function and Pathology
- Medicinal Plants and Bioactive Compounds
- Neuroscience and Neural Engineering
- Bioinformatics and Genomic Networks
- Dementia and Cognitive Impairment Research
KU Leuven
2019-2020
Belgian Nuclear Research Centre
2017-2020
Background and Purpose: Epidemiological data suggests an excess risk of cardiovascular disease (CVD) at low doses (0.05 0.1 Gy) ionizing radiation (IR). Furthermore, the underlying biological molecular mechanisms radiation-induced CVD are still unclear. Because damage to endothelium could be critical in IR-related CVD, this study aimed identify effects on immortalized endothelial cells context atherosclerosis. Material Methods: Microarrays RT-qPCR were used compare response irradiated with a...
Epigenetic clocks are based on age-associated changes in DNA methylation of CpG-sites, which can accurately measure chronological age different species.Recently, several studies have indicated that the difference between and epigenetic age, defined as acceleration, could reflect biological indicating functional decline diseases.In humans, an clock associated Alzheimer's disease (AD) pathology with acceleration age.In this study, we developed validated two mouse brain regionspecific from...
Therapeutic developments for neurodegenerative disorders are redirecting their focus to the mechanisms that contribute neuronal connectivity and loss thereof. Using a high-throughput microscopy pipeline integrates morphological functional measurements, we found inhibition of dual leucine zipper kinase (DLK) increased in primary cortical cultures. This neuroprotective effect was not only observed basal conditions but also cultures depleted from antioxidants which microtubule stability...
Background and Purpose. Up to 50 – 60% of all cancer patients receive radiotherapy as part their treatment strategy. However, the mechanisms accounting for increased vascular risks after irradiation are not completely understood. Mitochondrial dysfunction has been identified a potential cause radiation-induced atherosclerosis. Material Methods. Assays apoptosis, cellular metabolism, mitochondrial DNA content, functionality morphology were used compare response endothelial cells single 2 Gy...
Abstract Background Therapeutic developments for neurodegenerative disorders are redirecting their focus to the mechanisms that contribute synaptic plasticity and loss thereof. Identification of novel regulators requires a method quantify neuronal network connectivity with high accuracy throughput. To meet this demand, we have established microscopy-based pipeline integrates morphological functional correlates in primary culture. Results We unveiled signature was specific cell type culture...