A5057702206- Ubiquitin and proteasome pathways
- Fluorine in Organic Chemistry
- Epigenetics and DNA Methylation
- Retinoids in leukemia and cellular processes
- Organometallic Compounds Synthesis and Characterization
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Phytochemistry and Biological Activities
- Peroxisome Proliferator-Activated Receptors
- Nuclear Structure and Function
- Mercury impact and mitigation studies
- Histone Deacetylase Inhibitors Research
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Radiopharmaceutical Chemistry and Applications
- Boron and Carbon Nanomaterials Research
- Marine Biology and Environmental Chemistry
- Essential Oils and Antimicrobial Activity
- Adenosine and Purinergic Signaling
- Fire effects on ecosystems
- Glycosylation and Glycoproteins Research
- Magnetism in coordination complexes
- Energetic Materials and Combustion
- Inorganic Chemistry and Materials
- Amino Acid Enzymes and Metabolism
Université de Montréal
2014-2024
Beni-Suef University
2010-2024
Hokkaido University
2020
Rift Valley fever virus (RVFV) is a viral zoonosis that causes severe disease in ruminants and humans. The nonstructural small (NSs) protein the primary virulence factor of RVFV suppresses host's antiviral innate immune response. Bioinformatic analysis AlphaFold structural modeling identified four putative LC3-interacting regions (LIR) motifs (NSs 1-4) NSs protein, which suggest interacts with host LC3-family proteins. Using, isothermal titration calorimetry, X-ray crystallography,...
Context: Phytochemical investigation of Clerodendrum chinense (Osbeck) Mabberley (Lamiaceae) cultivated in Egypt and evaluation for anti-inflammatory, analgesic, antipyretic effects the methanol chloroform extracts chinense, indicum (L.) Kuntze, glabrum E. Meyer.Objective: The objective this study was to investigate species under investigation.Materials methods: Air-dried powdered leaves C. were extracted with MeOH 80%. This extract fractionated successive portions chloroform, ethyl acetate...
In bacterial resistance to mercury, the organomercurial lyase (MerB) plays a key role in detoxification pathway through its ability cleave Hg-carbon bonds. Two cysteines (C96 and C159; Escherichia coli MerB numbering) an aspartic acid (D99) have been identified as catalytic residues, these three residues are conserved all but four known variants, where is replaced with serine. To understand of active site serine, we characterized structure metal binding properties E. mutant serine...
The organomercurial lyase MerB has the unique ability to cleave carbon-Hg bonds, and structural studies indicate that three residues in active site (C96, D99, C159 E. coli MerB) play important roles bond cleavage. However, role of each residue carbon-metal cleavage not been well-defined. To do so, we have structurally biophysically characterized interaction with a series organotin organolead compounds. Studies two known inhibitors MerB, dimethyltin (DMT) triethyltin (TET), reveal they...
Chloroformic and methanolic extracts of four Clerodendrum species cultivated in Egypt were screened for antimicrobial activities. the flowers chinense splendens active against Plasmodium falciparum (IC50 < 10 µg mL−1). stem C. Trypanosoma cruzi = 1.21 1.12 mL−1, respectively) with marginal cytotoxicity. leaves showed promising activities T. 3.39 1.98 without cytotoxic effect on a human cell line. None selected plants significant activity Gram-negative or Gram-positive bacteria Candida...
SUMO proteins are important regulators of many key cellular functions in part through their ability to form interactions with other containing interacting motifs (SIMs). One characteristic feature all is the presence a highly divergent intrinsically disordered region at N-terminus. In this study, we examine role N-terminal SUMO-SIM required for formation nuclear bodies by promyelocytic leukemia (PML) protein (PML-NBs). We demonstrate that SUMO1 paralog specific manner as an auto-inhibition...
The p53 protein is a transcriptional regulatory factor and many of its functions require that it forms tetrameric structure. Although the tetramerization domain mammalian proteins (p53TD) share significant sequence similarities, was recently shown tree shrew p53TD considerably more thermostable than human p53TD. To determine whether other species display differences in this domain, we used biophysical, functional, structural studies to compare properties p53TDs from six model organisms...
The Atg8-family proteins (MAP1LC3/LC3A, LC3B, LC3C, GABARAP, GABARAPL1 and GABARAPL2) play a pivotal role in macroautophagy/autophagy through their ability to help form autophagosomes. Although autophagosomes the cytoplasm, nuclear levels of are significant. Recently, nuclear/cytoplasmic shuttling LC3B was shown require deacetylation two Lys residues (K49 K51 LC3B), which conserved proteins. To exit nucleus, deacetylated must bind TP53INP2/DOR (tumor protein p53 inducible 2) interaction with...
Select bacterial strains survive in mercury-contaminated environments due to acquisition of a transferable genetic element known as the mer operon. The operon typically encodes for series proteins that includes two enzymes, MerA and MerB. organomercurial lyase (MerB) cleaves carbon-mercury bonds compounds yielding ionic mercury Hg (II) reduced-carbon compound. ion product remains bounds until it is shuttled directly mercuric reductase (MerA) be reduced. Based on NMR spectroscopy X-ray...