A5034632870- Cellular transport and secretion
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- Wnt/β-catenin signaling in development and cancer
- Axon Guidance and Neuronal Signaling
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Neurological disorders and treatments
- Plant responses to water stress
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Advanced Fluorescence Microscopy Techniques
- Signaling Pathways in Disease
- Neuroscience and Neuropharmacology Research
University of Southern California
2015-2024
University of California, San Francisco
2022-2024
University of San Francisco
2022
Neural type-specific expression of clustered Protocadherin (Pcdh) proteins is essential for the establishment connectivity patterns during brain development. In mammals, deterministic same Pcdh isoform promotes minimal overlap tiled projections serotonergic neuron axons throughout brain, while stochastic genes allows convergence tightly packed, overlapping olfactory sensory into targeted structures. How can gene locus generate opposite transcriptional programs that orchestrate distinct...
The ability of neurons to rapidly remodel their synaptic structure and strength in response neuronal activity is highly conserved across species crucial for complex brain functions. However, mechanisms required elicit coordinate the acute, activity-dependent structural changes synapses are not well understood, as neurodevelopment plasticity tightly linked. Here, using an RNAi screen Drosophila against genes affecting nervous system functions humans, we uncouple cellular processes important...
Aggregation of huntingtin protein arising from expanded polyglutamine (polyQ) sequences in the exon-1 region mutant plays a central role pathogenesis Huntington's disease. The aggregation pathways are therapeutic and diagnostic interest, but obtaining critical information physiologically relevant htt (Httex1) has been challenging. Using biophysical techniques an expression purification protocol that generates clean, monomeric Httex1, we identified mapped three distinct pathways: 1) unseeded...
Expression of Protocadherin (Pcdh) genes is critical to the generation neuron identity and wiring nervous system. Pcdhα are arranged in clusters exhibit a range expression profiles, from stochastic deterministic. Because promoters have high sequence share distal enhancers, how distinct neurons choose which gene express remains unclear. We show that interplay between multiple epigenetics, genome folding orchestrates differential readouts locus across neurons. The probability promoter choice...
Abstract The ability of neurons to rapidly remodel their synaptic structure and strength in response neuronal activity is highly conserved across species crucial for complex brain functions. However, mechanisms required elicit coordinate the acute, activity-dependent structural changes synapses are not well understood. Here, using an RNAi screen Drosophila against genes affecting nervous system functions humans, we uncouple cellular processes important plasticity from synapse development. We...
Neurons use multiple modes of endocytosis, including clathrin-mediated endocytosis (CME) and activity-dependent bulk (ADBE), during mild intense neuronal activity, respectively, to maintain stable neurotransmission. While molecular players modulating CME are well characterized, factors regulating ADBE mechanisms coordinating activations remain poorly understood. Here we report that Minibrain/DYRK1A (Mnb), a kinase mutated in autism up-regulated Down’s syndrome, plays novel role suppressing...
ABSTRACT Clustered Protocadherin (Pcdh) proteins act as cell-surface recognition barcodes for neural circuit formation. Neurites expressing the same barcode repel each other, but this mechanism is deployed in two different ways. For instance, convergence of olfactory sensory neuron (OSN) projections requires stochastic expression distinct Pcdh isoforms individual cells, while tiling arbors serotonergic neurons (5-HTs) isoform, Pcdhαc2. Despite their essential role, however, molecular...