A5059105109- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Urticaria and Related Conditions
- Autoimmune Bullous Skin Diseases
- RNA modifications and cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Mast cells and histamine
- Dermatology and Skin Diseases
- Genomics and Chromatin Dynamics
- Ion Transport and Channel Regulation
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- Paleontology and Stratigraphy of Fossils
- Allergic Rhinitis and Sensitization
- Genetic Syndromes and Imprinting
- Diet and metabolism studies
- Enzyme function and inhibition
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Methemoglobinemia and Tumor Lysis Syndrome
- RNA Interference and Gene Delivery
- Porphyrin Metabolism and Disorders
- Single-cell and spatial transcriptomics
- Protein Kinase Regulation and GTPase Signaling
- Axon Guidance and Neuronal Signaling
Humboldt-Universität zu Berlin
2022-2025
Charité - Universitätsmedizin Berlin
2022-2025
Freie Universität Berlin
2022-2025
Fraunhofer Institute for Translational Medicine and Pharmacology
2022-2025
University of California, San Francisco
2021-2024
Yale University
2018-2023
University of San Francisco
2022
Amt für Umwelt
2017
University of San Diego
2015
Abstract Background Chronic inducible urticaria (CIndU) is characterized by mast cell (MC)‐mediated wheals in response to triggers: cold (ColdU) and friction symptomatic dermographism (SD). KIT receptor activation stem factor (SCF) essential for MC function. Barzolvolimab (CDX‐0159) a humanized antibody that inhibits SCF was well tolerated healthy volunteers with dose‐dependent plasma tryptase suppression indicative of systemic ablation. Methods This an open‐label, trial patients...
Neural type-specific expression of clustered Protocadherin (Pcdh) proteins is essential for the establishment connectivity patterns during brain development. In mammals, deterministic same Pcdh isoform promotes minimal overlap tiled projections serotonergic neuron axons throughout brain, while stochastic genes allows convergence tightly packed, overlapping olfactory sensory into targeted structures. How can gene locus generate opposite transcriptional programs that orchestrate distinct...
Expression of Protocadherin (Pcdh) genes is critical to the generation neuron identity and wiring nervous system. Pcdhα are arranged in clusters exhibit a range expression profiles, from stochastic deterministic. Because promoters have high sequence share distal enhancers, how distinct neurons choose which gene express remains unclear. We show that interplay between multiple epigenetics, genome folding orchestrates differential readouts locus across neurons. The probability promoter choice...
RNA-sequencing (RNA-seq) measures RNA abundance in a biological sample but does not provide temporal information about the sequenced RNAs. Metabolic labeling can be used to distinguish newly made RNAs from pre-existing Mutations induced chemical recoding of hydrogen bonding pattern metabolic label reveal which are new context sequencing experiment. These nucleotide strategies have been developed for single uridine analogue, 4-thiouridine (s4U), limiting scope these experiments. Here we...
Importance Treating patients with chronic urticaria using omalizumab has been shown to be safe and effective in randomized clinical trials. Multinational studies on long-term performance practice settings are lacking, especially drug survival. Drug survival, which refers the length of time that treated a specific drug, is comprehensive outcome covering effectiveness, safety, patient physician preferences. Furthermore, little known about reasons potential predictors for discontinuation....
ABSTRACT Background Afamelanotide 16 mg (SCENESSE) is the first approved treatment for erythropoietic protoporphyria (EPP). EPP a rare autosomal recessive inherited disorder of haem biosynthesis pathway, where patients experience severe and debilitating acute phototoxicity. It affects at least one in 140,000 European population. A postauthorisation safety study (PASS) disease registry were imposed as conditions marketing authorisation. Objectives Evaluate short‐ long‐term clinical...
RNA metabolic labeling using 4-thiouridine (s
Abstract Quantitative comparisons of RNA levels from different samples can lead to new biological understanding if they are able distinguish variation variable sample preparation. These challenges pronounced in that require complex biochemical manipulations (e.g. isolating polysomes study translation). Here, we present Transcript Regulation Identified by Labeling with Nucleoside Analogues Cell Culture (TILAC), an internally controlled approach for quantitative content. TILAC uses two...
ABSTRACT Clustered Protocadherin (Pcdh) proteins act as cell-surface recognition barcodes for neural circuit formation. Neurites expressing the same barcode repel each other, but this mechanism is deployed in two different ways. For instance, convergence of olfactory sensory neuron (OSN) projections requires stochastic expression distinct Pcdh isoforms individual cells, while tiling arbors serotonergic neurons (5-HTs) isoform, Pcdhαc2. Despite their essential role, however, molecular...
Chronic inducible urticaria (CIndU) is defined by pruritic wheal formation and/or angioedema in response to a definite and specific trigger.1, 2 CIndUs account for 20–30% of all cases chronic urticaria, that is, lasts longer than 6 weeks. Wheals angioedema, most types CIndU, usually occur within 10 min after exposure the trigger resolve 1–3 h cessation exposure, except delayed pressure (DPU), where symptoms do not appear until hours last up 24 h. CIndU subtypes are characterized classified...
Abstract Background Long‐term data on the effectiveness and safety of omalizumab for chronic inducible urticaria (CIndU) in large populations are lacking. Objective To evaluate effectiveness, safety, estimated treatment duration its predictors, as well differences between CIndU subtypes, a long‐term cohort. Methods A multinational multicenter study was conducted at 14 specialized centres (UCAREs), including all patients ever treated with from 2009 until July 2022. Kaplan–Meier survival...
Abstract Somatic mutations in splicing factors are of significant interest myeloid malignancies and other cancers. U2AF1, together with U2AF2, is essential for 3’ splice site recognition. U2AF1 result aberrant splicing, but the molecular mechanism full spectrum consequences on RNA biology have not been fully elucidated to date. We performed multi-omics profiling vivo binding, turnover S34F Q157R mutants. dissected specific binding signals U2AF2 showed that individually alter U2AF1-RNA...