A5079022589- Ion Transport and Channel Regulation
- Genetics, Bioinformatics, and Biomedical Research
- Pancreatic function and diabetes
- Biomedical and Engineering Education
- Protein Kinase Regulation and GTPase Signaling
- Enzyme function and inhibition
- Signaling Pathways in Disease
- Various Chemistry Research Topics
- Innovative Teaching Methods
- Ion channel regulation and function
- Career Development and Diversity
- Enzyme Structure and Function
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Hydrogen Storage and Materials
- ATP Synthase and ATPases Research
- Mechanisms of cancer metastasis
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Biochemical Analysis and Sensing Techniques
- Metabolism, Diabetes, and Cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Phosphodiesterase function and regulation
- Plant nutrient uptake and metabolism
- Protease and Inhibitor Mechanisms
University of San Diego
2016-2025
Southwestern Medical Center
2024
City of Hope
2024
Beckman Research Institute
2024
Bridge University
2024
Alabama Space Grant Consortium
2024
Mercyhurst University
2024
The University of Texas Southwestern Medical Center
2024
Universidad del Noreste
2023
Minnesota State University Moorhead
2005-2016
The regulation of phospholipase D cloned from rat brain (rPLD) was examined in vivo andin vitro. enzyme a shorter splice variant human 1 (Hammond, S. M., Altshuller, Y. Sung, T.-C., Rudge, Rose, K., Engebrecht, J. A., Morris, A. J., and Frohman, M. (1995) Biol. Chem. 270, 29640–29643). Its expression COS-7 cells led to increased (PLD) activity that further stimulated by constitutively active V14RhoA. V14RhoA had no effect on the endogenous PLD cells, but L71ARF3 its activity. In contrast,...
Phospholipase D (PLD) is regulated by many factors including the small G-proteins, RhoA and ADP-ribosylation factor (ARF). The present study examined distribution of RhoA- ARF-responsive PLD in membranes, microsomes cytosol rat tissues liver subcellular fractions. was all tissue fractions stimulated guanosine 5'-[gamma-thio]triphosphate (GTP[S]), with highes: specific activities being lung, kidney spleen. When myristoylated recombinant ARF (mARF) added GTP[S], activity further, but addition...
Course-based Undergraduate Research Experiences (CUREs) have beneficial impacts on students and the capacity to provide authentic research experiences that are accessible all students, especially those from Minoritized Groups. CUREs can be presented in a full semester format (cCURE) shorter modules incorporated into laboratory courses (mCURE). In this study, protein-centric were implemented at two minority-serving Community Colleges (CCs) introductory biology chemistry courses. Using...
Background/Objectives: Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by excessive extracellular matrix (ECM) production and tissue stiffening, resulting in impaired function. Sodium hydrogen exchanger isoform 1 (NHE1) key mediator of intracellular pH regulation, influencing fibroblast activation, motility, proliferative pathways. This study investigates the role NHE1 actin stress fiber formation, fibroblast-to-myofibroblast differentiation, cytokine secretion...
Research-based learning in a teaching environment is an effective way to help bring the excitement and experience of independent bench research large number students. The program described here second two-semester biochemistry laboratory series. Here, students are empowered design, execute analyze their own experiments for entire semester. This style replaces variety shorter labs favor depth research-based experience. concept allow function groups. projects focused on series wild-type mutant...
A successful laboratory experience provides the foundation for student success, creating active participation in learning process. Here, we describe a new approach that emphasizes research, inquiry and problem solving year-long biochemistry experience. The first semester centers on purification, characterization, analysis of novel fusion protein within guided research Throughout semester, students gradually acquire skills as they are allowed to work independently. protein, malate...
Over the past two years, through an NSF RCN UBE grant, ASBMB has held regional workshops for faculty members from around country. The have focused on developing lists of Core Principles or Foundational Concepts in Biochemistry and Molecular Biology, a list foundational skills, concepts Physics, Chemistry, Mathematics that all Biology majors must understand to complete their major coursework. allied fields working group created survey validate identified participant feedback at various...
Course-based undergraduate research experiences (CUREs) are laboratory courses that integrate broadly relevant problems, discovery, use of the scientific process, collaboration, and iteration to provide more students with than is possible in individually mentored faculty laboratories. Members national Malate dehydrogenase CUREs Community (MCC) investigated differences student impacts between traditional (control), a short module CURE within (mCURE), lasting entire course (cCURE). The sample...
Undergraduate research is an important experience that increases retention, ownership, and learning. Integrating experiences into the classroom focus of course-based undergraduate education (CUREs) has become tool in curricula many universities. This article will serve as a guide for those interested starting CURE. Information on makeup CUREs suggested workflow to design CURE chemistry disciplines described. Examples recent focused examples introduction supported networks are described help...
The COVID-19 pandemic has challenged undergraduate instructors and students in an unprecedented manner.
ADP-ribosylation factors (ARFs) regulate coatomer assembly on the Golgi as well recruitment of clathrin adapter proteins and are therefore involved in vesicle budding from vesicular transport. They also regulators phospholipase D (PLD) activity. Arfaptin 1 is an ARF binding protein that inhibits PLD activation, trafficking secretion. In present report, we show arfaptin interacts with 'high speed' membranes independently ARF. However, addition myristoylated ARF3 (myrARF3) increases...
The implementation of course-based undergraduate research experiences (CUREs) has made it possible to expose large populations experiences. For these be authentic, they should reflect the increasingly collaborative nature research. While some CUREs have expanded, involving multiple schools across nation, is still unclear how a structured extramural collaboration between students and faculty from an outside institution affects student outcomes. In this study, we established three cohorts...