A5102978547- Cancer, Lipids, and Metabolism
- Fatty Acid Research and Health
- Cancer, Hypoxia, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Mast cells and histamine
- Lipid metabolism and biosynthesis
- Eicosanoids and Hypertension Pharmacology
- Cancer-related Molecular Pathways
- Fungal and yeast genetics research
- Neuroscience and Neuropharmacology Research
- PI3K/AKT/mTOR signaling in cancer
- NF-κB Signaling Pathways
- 14-3-3 protein interactions
- Immune Cell Function and Interaction
- Ion channel regulation and function
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Nerve injury and regeneration
- Asthma and respiratory diseases
- Mitochondrial Function and Pathology
- Inflammatory mediators and NSAID effects
- Cell death mechanisms and regulation
- Pediatric health and respiratory diseases
- Congenital heart defects research
- TGF-β signaling in diseases
Chungnam National University
2011-2023
National University College
2020
New Generation University College
2020
National Heart Lung and Blood Institute
2008-2009
National Institutes of Health
2008-2009
University of Pittsburgh
2008
Vanderbilt University
1997-1998
Howard Hughes Medical Institute
1997
Korea Research Institute of Bioscience and Biotechnology
1996
Docosahexaenoic acid (DHA) has been reported to induce tumor cell death by apoptosis. However, little is known about the effects of DHA on autophagy, another complex well-programmed process characterized sequestration cytoplasmic material within autophagosomes. Here, we show that increased both level microtubule-associated protein light-chain 3 and number autophagic vacuoles without impairing vesicle turnover, indicating induces not only apoptosis but also autophagy. We observed DHA-induced...
Docosahexaenoic acid (DHA) induces autophagy-associated apoptotic cell death in wild-type p53 cancer cells via regulation of p53. The present study investigated the effects DHA on PC3 and DU145 prostate lines harboring mutant Results show that, addition to apoptosis, increased expression levels lipidated form LC3B potently stimulated autophagic flux, suggesting that both autophagy apoptosis expressing led generation mitochondrial reactive oxygen species (ROS), as shown by ROS-specific probe...
The regulation of phospholipase D cloned from rat brain (rPLD) was examined in vivo andin vitro. enzyme a shorter splice variant human 1 (Hammond, S. M., Altshuller, Y. Sung, T.-C., Rudge, Rose, K., Engebrecht, J. A., Morris, A. J., and Frohman, M. (1995) Biol. Chem. 270, 29640–29643). Its expression COS-7 cells led to increased (PLD) activity that further stimulated by constitutively active V14RhoA. V14RhoA had no effect on the endogenous PLD cells, but L71ARF3 its activity. In contrast,...
The anticancer properties and mechanism of action omega-3 polyunsaturated fatty acids ( ω 3-PUFAs) have been demonstrated in several cancers; however, the lung cancer remains unclear. Here, we show that docosahexaenoic acid (DHA), a 3-PUFA, induced apoptosis autophagy non-small cell (NSCLC) cells. DHA-induced death was accompanied by AMP-activated protein kinase (AMPK) activation inactivated phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target rapamycin (mTOR) signaling. Knocking down...
G13 belongs to the G12 family of heterotrimeric G proteins, whose effectors are poorly defined. The present study was designed test if phospholipase D (PLD) is regulated by and Rho-type small GTPases involved. Expression constitutively active Q226L mutant α-subunit in COS-7 cells stimulated activity a rat brain isozyme (rPLD1) co-expressed cells. Wild type Gα13 ineffective unless were incubated with AlF4−. rPLD1 previously shown be activated V14RhoA (Park, S. K., Provost, J. J., Bae, C. D.,...
The role of omega-3 polyunsaturated fatty acids (ω3-PUFAs) in cancer prevention has been demonstrated; however, the exact molecular mechanisms underlying anticancer activity ω3-PUFAs are not fully understood. Here, we investigated relationship between action a specific ω3-PUFA docosahexaenoic acid (DHA), and conventional mitogen-activated protein kinases (MAPKs) including extracellular signal-regulated kinase (ERK), c-JUN N-terminal (JNK) p38 whose dysregulation implicated human cancers.MTT...
Although preconditioning injury on the peripheral nerve induces axonal regenerative capacity in neurons, it is not known whether similar lesion effects occur glial cells. Here we demonstrate that Schwann cells are activated by preinjury and primed to mediate axon regeneration. Cdc2, which was induced from after sciatic injury, phosphorylated vimentin almost exclusively distal area. Phospho-vimentin-positive showed increased migration activity were close contact with process outgrowth of...
Abstract Background RET/PTC (rearranged in transformation/papillary thyroid carcinomas) gene rearrangements are the most frequent genetic alterations identified papillary carcinoma. Although it has been established that kinase plays a crucial role intracellular signaling pathways regulate cellular transformation, growth, and proliferation epithelial cells, upstream leads to activation of is largely unknown. Based on observation high levels PLD expression human cancer tissues, we investigated...
Abstract: Phospholipase D2 (PLD2) is expressed in brain andinhibited by synuclein, which involved Parkinson's and Alzheimer'sdiseases. However, the activation mechanism of PLD2 neuronal cells has notbeen defined clearly. Hydrogen peroxide (H 2 O ) playsroles neurodegenerative diseases also acts as a second messenger ofvarious molecules such nerve growth factor. To study regulation mechanismsof H cells, we have made stablePC12 cell lines expressing (PLD2‐PC12 cells). treatment stimulated PLD...
Pervanadate, a complex of vanadate and H2O2, has an insulin mimetic effect, acts as inhibitor protein tyrosine phosphatase. Pervanadate-induced phospholipase D (PLD) activation is known to be dependent on the phosphorylation cellular proteins kinase C (PKC) activation, yet underlying molecular mechanisms are not clearly understood. Here, we investigated signaling pathway pervanadate-induced PLD in Rat2 fibroblasts. Pervanadate increased activity dose- time- manner. Protein inhibitor,...
A nucleobase adenine is a fundamental component of nucleic acids and nucleotides. Various biological roles have been discovered. It not produced from degradation nucleotides in mammals but mainly during polyamine synthesis by dividing cells. Anti-inflammatory supported IgE-mediated allergic reactions, immunological functions lymphocytes dextran sodium sulfate-induced colitis. However effects on Toll-like receptor 4 (TLR4)-mediated inflammation lipopolysaccharide (LPS), cell wall Gram...
비만세포는 알레르기 반응을 일으키는 주된 세포로서 항원 자극에 반응하여 유발 물질인 히스타민, 단백질 분해효소, 염증성 지질 물질 및 사이토카인 등을 분비한다. 아미노산인 트립토판은 염증반응을 조절한다. 트립토판 투여는 비만세포가 관여하는 장염 증상을 완화시킨다. 그러나 트립토판이 비만세포의 반응에 미치는 영향에 대한 연구는 없다. 본 저자들은 IgE 매개 영향을 비만세포 수준에서 그리고 실험동물 생쥐에서 연구하였다. IgE-매개 수동 피부 아나필락시스를 또한 활성화 반응 즉, 탈과립 반응, 지질인 LTB4와 사토카인 (TNF-α와 IL-4) 등의 분비를 측정하였다. 트립토판을 생쥐에 복강 주사하면 억제하였다. 수준에서도 반응들, 즉 반응과 LTB4 사이토카인인 TNF-α와 IL4의 이러한 결과로부터 세포 수준 억제함을 알 수 있었다.
The protooncogene c-myc is known to be associated with both cell proliferation and apoptosis. possible cellular affects of castration on the ventral prostate gland rat as well relationship a induced expression were examined. Levels mRNA in peaked at 6 h (early induction) 48 (late after castration, respectively. Castration-induced DNA fragmentation was not observed an early induction mRNA. appeared testosterone-dependent. On other hand, synthesis measured by [3H]thymidine uptake increased...
대장암은 미국 등 서양 국가뿐만 아니라 국내에서도 2번째로 많이 발병이 되는 암으로 알려져 있다. 역학조사에 의하면 <TEX>${\omega}3$</TEX>-PUFAs를 섭취한 인종에서 대장암 발생빈도가 감소하고 최근 <TEX>${\omega}3$</TEX>-PUFAs는 수종의 암에 대해 항암작용을 나타낸다고 한다. 이에 본 연구에서는 대장암에서 DHA <TEX>${\omega}3$</TEX>-PUFA의 항침윤 기전을 규명하여 다음과 같은 결과를 얻었다. DHA및 EPA는 세포주 SW480의 증식을 농도 의존적으로 억제하였으나 AA는 거의 영향이 없었으며 TUNEL assay로 apoptotic cell death가 확인 되었다. DHA는 <TEX>$\beta$</TEX>-catenin 단백 및 TCF/LEF luciferase 활성을 억제 하였다. SW480 세포의 침윤능은 DHA의 농도에 억제되었다. DHA처리 후 MMP-9 MMP-2 mRNA양이 감소되었을 뿐만 그...
비만세포는 알레르기를 유발하는 중요한 세포로서 항원의 자극을 받으면 알레르기 유발 물질을 분비한다. 히스티딘은 비만세포가 관여하는 만성 장염 증세를 완화하는 기능이 있다. 그러나 히스티딘이 비만세포의 활성화에 미치는 영향에 대한 연구는 없다. 우리는 반응 연구의 동물모델인 IgE 매개 수동 피부 아나필락시스 방법 그리고 분비하는 반응(탈과립 반응, 염증성 지질 및 사이토카인 분비)을 통해 알레르기에 영향을 연구하였다. 생쥐의 복강으로 히스티딘을 100 mg/kg으로 투여하면 아나필락시스를 통계적으로 의미 있는 수준으로 억제하였고, 비만세포에서도 탈과립 반응과 불질의 분비를 억제하였다. 이러한 결과는 히스티딘 섭취는 억제하는 데 유익할 것으로 생각할 수