A5101470904- Liver physiology and pathology
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Cellular Mechanics and Interactions
- Fuel Cells and Related Materials
- Bioactive Compounds and Antitumor Agents
- Migraine and Headache Studies
- Polyamine Metabolism and Applications
- Bone Tissue Engineering Materials
- Granular flow and fluidized beds
- Microbial Natural Products and Biosynthesis
- Lanthanide and Transition Metal Complexes
- Ophthalmology and Eye Disorders
- Fluid Dynamics and Mixing
- Surface Roughness and Optical Measurements
- Mesenchymal stem cell research
- Nanoparticles: synthesis and applications
- Enzyme-mediated dye degradation
- CCD and CMOS Imaging Sensors
- Vehicle emissions and performance
- Polar Research and Ecology
- Spacecraft Design and Technology
- Pluripotent Stem Cells Research
Korea Research Institute of Bioscience and Biotechnology
1999-2023
Clemson University
2014-2015
Intelligent Image and Information System
2014
Yokohama National University
2011-2013
Yeungnam University
2012
Yonsei University Health System
2010
Korea Institute of Ocean Science and Technology
2008-2009
Kookmin University
2005
Aptiv (United States)
2004
Abstract We fabricated a spheroid-forming unit (SFU) for efficient and economic production of cell spheroids. optimized the protocol generating large homogenous liver cancer spheroids using Huh7 hepatocellular carcinoma (HCC) cells. The showed apoptotic proliferative signals in centre at surface, respectively. In particular, hypoxia-induced factor-1 alpha (HIF-1α) ERK signal activation were detected To diminish core necrosis increase oncogenic character, we co-cultured with 2% human...
In this paper, we propose a new computational method for efficient evaluation of the fluid mixing behaviour in T-shaped micromixer with rectangular cross section at high Schmidt number under steady state conditions. Our approach enables low-cost high-quality simulation based on tracking particles convective and posterior solving model species equation molecular diffusion. The examined parameter range is Re = 1.33 × 10(-2) to 240 Sc 3600. proposed shown simulate well quality even engulfment...
Current in vitro liver models provide three-dimensional (3-D) microenvironments combination with tissue engineering technology and can perform more accurate vivo mimicry than two-dimensional models. However, a human cell-based, functionally mature model is still desired, which would an alternative to animal experiments resolve low-prediction issues on species differences. Here, we prepared hybrid hydrogels of varying elasticity compared them normal liver, develop that preserves properties...
Abstract Hepatic stellate cells (HSCs) play an important role in liver fibrosis; however, owing to the heterogeneity and limited supply of primary HSCs, development vitro fibrosis models has been impeded. In this study, we established characterized a novel human HSC line (LSC‐1), applied it various types three‐dimensional (3D) co‐culture systems with differentiated HepaRG cells. Furthermore, compared LSC‐1 commercially available on conventional monolayer culture. exhibited overall...
Two novel bacterial strains, designated DOKDO 025T and 023T, were isolated on Dokdo Island, Korea, from the rhizosphere of brown alga Ecklonia kurome. The strains subjected to a polyphasic taxonomy study found be Gram-negative, aerobic, rod-shaped, non-motile orange-coloured. isolates shared 96.3 % 16S rRNA gene sequence similarity. They showed 93.8–95.6 similarity with respect members genus Muricauda in family Flavobacteriaceae, but formed distinct phyletic line. Moreover, cellular...
Two heterotrophic, aerobic, yellow-pigmented, Gram-negative, non-gliding bacteria, designated AKS293T and AKS432T, isolated from a red alga, were analysed using polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed that the novel strains affiliated to genus Lacinutrix, member of family Flavobacteriaceae, showing similarities 96.1–96.4 % with respect type strain Lacinutrix copepodicola. The two isolates shared 99.5 similarity 55.0 DNA–DNA relatedness. They grew optimally at...
A novel strain, designated MRN461T, was isolated from a marine sponge in Micronesia. The 16S rRNA gene sequence of the isolate showed 95.6 % similarity with that 'Microscilla furvescens' IFO (now NBRC) 15994. Phylogenetic analysis revealed and 15994 formed distinct phyletic line within family 'Flexibacteraceae'. Cells strain MRN461T were Gram-negative, long filamentous rods, motile by gliding. Growth observed at 15–40 °C (optimum, 33 °C), pH 5.0–9.5 7.5) presence 0.5–7.0 sea salts 2.5 %)....
Abstract Although primary human hepatocytes (PHHs) are the gold standard in drug efficacy and metabolism studies, long‐term survival of PHHs maintenance their hepatic function still challenging. In this study, we focused on effect initial microenvironment upregulation preservation encapsulated within biodegradable hydrogel systems. were RGD‐functionalized hybrid hydrogels with various degrees degradability, functionality was analyzed. Regardless elastic modulus, combination nondegradable had...
Non-alcoholic fatty liver disease (NAFLD) begins as simple hepatic steatosis, but further progress to chronic diseases results in severe damage and failure. However, therapeutic options are scarce due the lack of reliable human vitro models for understanding progression mechanisms developing therapies.We describe here a novel method generating 3D spheroids using HepaRG cells, vascular endothelial mesenchymal stem cells cultured on thick layer soft matrix narrow conical tube; this improved...
Scaffold-based delivery of bioactive molecules capable directing stem cell differentiation is critical to the development point-of-care therapy for orthopedic repair. Dexamethasone-conjugated hyaluronic acid (HA-DXM) was synthesized and combined with hydrolytically degradable, photo-cross-linkable PEG-bis(2-acryloyloxy propanoate) (PEG-bis-AP) form semi-IPNs. Dexamethasone (DX) release limited in physiological buffer substantially increased presence encapsulated human mesenchymal cells...
Abstract Spheroids, a widely used three‐dimensional (3D) culture model, are standard in hepatocyte as they preserve long‐term functionality and enhance survivability. In this study, we investigated the effects of three operation modes 3D — static, orbital shaking, under vertical bidirectional flow using spheroid forming units (SFUs) on hepatic differentiation drug metabolism to propose best for mass production functionally enhanced spheroids. Spheroids SFUs exhibited increased gene...
Tumor cells overexpress amino acid transporters to meet the increased demand for acids. PQ loop repeat‐containing ( PQLC )2 is a cationic transporter that might be involved in cancer progression. Here, we show upregulation of 2 critical gastric GC ) development vitro and vivo. Both mRNA protein were overexpressed tissues, especially diffuse type. Overexpression promoted cell growth, anchorage independence, tumor formation nude mice. This was due activation MEK / ERK 1/2 PI 3K/ AKT signaling....
An elastodynamic analysis of an interface crack in coated media with functionally graded properties is performed under the condition antiplane shear impact. The material exists as a nonhomogeneous interlayer between dissimilar, homogeneous phases coating/substrate system or coating deposited on substrate. nonhomogeneity represented terms power-law variations modulus and mass density. Based use integral transform technique, formulation transient problem reduced to having solve Cauchy-type...
The driveability of a vehicle is important to both drivers and manufacturers. Good can provide with better driving experience result in lower emissions. closely related the volatility fuel used, which characterized by index (DI). A sensor DI has been fabricated tested on vehicle. One version metal an interdigitated cube structure. element located vapor dome tank bathed while pump on. After turned off, reproducible volume retained between capacitor plates element. heats sample, causing it...
This paper shows the current status of cooled IR detector technologies at i3system, South Korea. Mass production technology i3system has successfully supplied lots QVGA detectors to camera customers. also developed small pitch with 320×256 and 640×512 formats for several different applications such as thermal sights 24-hour operation observation units. In 2013, i3system's been launched in STSAT(Science Technology SATellite)-2C through Naro-1 program which was Korea's first successful launch...