A5029697536- Metabolomics and Mass Spectrometry Studies
- Nutrition and Health in Aging
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Sphingolipid Metabolism and Signaling
- Mass Spectrometry Techniques and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Adipose Tissue and Metabolism
- Gut microbiota and health
- Pulmonary Hypertension Research and Treatments
- Analytical Chemistry and Chromatography
- Liver Disease Diagnosis and Treatment
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Air Quality and Health Impacts
- Diet and metabolism studies
- Erythrocyte Function and Pathophysiology
- Advanced Proteomics Techniques and Applications
- Health, Environment, Cognitive Aging
- Body Composition Measurement Techniques
- Diet, Metabolism, and Disease
- Digestive system and related health
- Food Allergy and Anaphylaxis Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cell Image Analysis Techniques
University of Ulsan
2015-2024
Asan Medical Center
2015-2024
Ulsan College
2015-2024
Korea Basic Science Institute
2024
Korea Institute of Oriental Medicine
2024
Hallym University
2024
Lee University
2024
Soonchunhyang University
2024
Yonsei University
2024
Seoul National University
2015-2019
A Western diet comprising high fat, carbohydrate, and low fiber content has been suggested to contribute an increased prevalence of colitis. To clarify the effect dietary cellulose (an insoluble fiber) on gut homeostasis, for 3 months mice were fed a high-cellulose (HCD) or low-cellulose (LCD) based AIN-93G formulation. Histologic evaluation showed crypt atrophy goblet cell depletion in colons LCD-fed mice. RNA-sequencing analysis higher expression genes associated with immune system...
Objective Lipotoxic hepatocyte injury is a primary event in non-alcoholic steatohepatitis (NASH), but the mechanisms of lipotoxicity are not fully defined. Sphingolipids and free cholesterol (FC) mediate injury, their link NASH has been explored. We examined role sphingomyelin synthases (SMSs) that generate (SM) diacylglycerol (DAG) pyroptosis, specific form programmed cell death associated with inflammasome activation, NASH. Design Wild-type C57BL/6J mice were fed high fat diet (HFHCD) to...
MicroRNA is an endogenous, small RNA controlling multiple target genes and playing roles in various biological processes including tumorigenesis. Here, we addressed the function of miR-155 using LC-MS/MS-based metabolic profiling deficient breast cancer cells. Our results revealed loss hampers glucose uptake glycolysis, via down-regulation transporters enzymes HK2, PKM2, LDHA. We showed this due to cMYC, controlled through phosphoinositide-3-kinase regulatory subunit alpha...
Lipid droplets are specialized cellular organelles that contain neutral lipid metabolites and play dynamic roles in energy homeostasis. Perilipin 1 (Plin1), one of the major droplet-binding proteins, is highly expressed adipocytes. In mice, Plin1 deficiency impairs peripheral insulin sensitivity, accompanied with reduced fat mass. However, mechanisms underlying resistance lean knockout (Plin1-/-) mice largely unknown. The current study demonstrates promotes inflammatory responses lipolysis...
Abstract Dietary fiber functions as a prebiotic to determine the gut microbe composition. The microbiota influences metabolic and immune responses in human health. metabolites produced by various dietary components not only modulate immunity but also impact organs. Although recent findings have suggested that microbial dysbiosis is associated with several respiratory diseases, including asthma, cystic fibrosis, allergy, role of nutrients respect pulmonary disease remains unclear. Therefore,...
Free cholesterol (FC) accumulation in the liver is an important pathogenic mechanism of nonalcoholic steatohepatitis (NASH). Plasmalogens, key structural components cell membrane, act as endogenous antioxidants and are primarily synthesized liver. However, role hepatic plasmalogens metabolic disease unclear. In this study, we found that levels docosahexaenoic acid (DHA)‐containing plasmalogens, expression glyceronephosphate O‐acyltransferase ( Gnpat ; rate‐limiting enzyme plasmalogen...
Macroautophagy (autophagy) is believed to maintain energy homeostasis by degrading unnecessary cellular components and molecules. Its implication in regulating cancer metabolism recently started be uncovered. However, the precise roles of autophagy are still unclear. Here, we show that plays a critical role glutamine metabolism, which required for tumor survival. Pancreatic ductal adenocarcinoma (PDAC) cells require both typical transporters intracellular levels. Glutamine deprivation, but...
Studies in aged mice support a role for kynurenine, tryptophan metabolite, age-induced bone loss; however, the of kynurenine human metabolism is not well understood.To assess whether level marrow (BM) aspirates, directly reflecting microenvironment, associated with osteoporosis-related phenotypes and biochemical markers.A case-control study conducted clinical unit.BM samples were collected from 72 patients at time hip surgery either fragility fracture (HF) (n = 27) or other causes 45). In...
Abstract Transmembrane B cell lymphoma 2-associated X protein inhibitor motif-containing (TMBIM) 6, a Ca 2+ channel-like protein, is highly up-regulated in several cancer types. Here, we show that TMBIM6 closely associated with survival patients cervical, breast, lung, and prostate cancer. deletion or knockdown suppresses primary tumor growth. Further, mTORC2 activation by stimulates glycolysis, synthesis, the expression of lipid synthesis genes glycosylated proteins. Moreover, ER-leaky from...
Epoxyeicosatrienoic acids (EETs) are metabolites of arachidonic acid that rapidly metabolized into diols by soluble epoxide hydrolase (sEH). sEH inhibition has been shown to increase the biological activity EETs, which known have anti-inflammatory properties. However, role EETs in pulmonary fibrosis remains unexplored. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used analyze lung tissues patients idiopathic (IPF, n = 29) and controls (n 15), function 11,12-EET...
Abstract Background Acute respiratory distress syndrome (ARDS) is etiologically and clinically a heterogeneous disease. Its diagnostic characteristics subtype classification, the application of these features to treatment, have been considerable interest. Metabolomics becoming important for identifying ARDS biology distinguishing its subtypes. This study aimed identify metabolites that could distinguish sepsis-induced patients from non-ARDS controls, using targeted metabolomics approach,...
Abstract Proper placental development in early pregnancy ensures a positive outcome later on. The developmental relationship between the placenta and embryonic organs, such as heart, is crucial for normal pregnancy. However, mechanism through which influences of organs remains unclear. Trophoblasts fuse to form multinucleated syncytiotrophoblasts (SynT), primarily make up materno-fetal interface. We discovered that endogenous progesterone immunomodulatory binding factor 1 (PIBF1) vital...
Abstract Food allergy (FA) is a widespread issue, affecting as many 10% of the population. Over past two to three decades, prevalence FA has been on rise, particularly in industrialized and westernized countries. complex, multifactorial disease mediated by type 2 immune responses involving environmental genetic factors. However, precise mechanisms remain inadequately understood. Metabolomics potential identify endotypes, which could beneficially promote personalized prevention treatment. A...
The generation of gaseous polyanions with a Coulomb barrier has attracted attention as exemplified by previous studies fullerene dianions. However, this phenomenon not been reported for biological anions. By contrast, electron attachment to multiply charged peptide and protein cations seen surge interest due the high utility tandem mass spectrometry (MS/MS). Electron capture dissociation (ECD) transfer (ETD) involve radical-driven fragmentation charge-reduced peptide/protein yield N-C(α)...
There is growing appreciation that estrogen signaling pathways can be modulated by naturally occurring environmental compounds such as phytoestrogens and the more recently discovered xenoestrogens. Many researchers studying effects of estrogens on brain function or behavior in animal models choose to use phytoestrogen-free food for this reason. Corncob bedding commonly used facilities across United States has been shown inhibit estrogen-dependent reproductive rats. The mechanism effect was...
Neutrophils can be induced to release DNA combined with histones. The resulting neutrophil extracellular trap (NET) provides a scaffold for growing hemostatic plug. Therefore, the NET formation may inevitable in clinical conditions that are characterized by of vascular thrombi. Thus far, there have been no reports on significance disseminated intravascular coagulation (DIC). we investigated circulating levels DIC and analyzed their potential values assess severity predict outcome.The plasma...
In the brain, seizures lead to release of large amounts polyunsaturated fatty acids including arachidonic acid (ARA). ARA is a substrate for three major enzymatic routes metabolism by cyclooxygenase, lipoxygenase and cytochrome P450 enzymes. These enzymes convert potent lipid mediators prostanoids, leukotrienes epoxyeicosatrienoic (EETs). The prostanoids are largely pro-inflammatory molecules that sensitize neurons whereas EETs anti-inflammatory reduce excitability neurons. Recent evidence...
The high circulating levels of histones found in various thrombotic diseases may compromise the anticoagulant barrier endothelial cells. We determined how affect procoagulant tissue factor (TF) and thrombomodulin (TM). Surface antigens, soluble forms, mRNA TF TM were measured by flow cytometry, ELISA, real-time RT-PCR, respectively. activity using activity, thrombin generation, or chromogenic assays. Involvement toll-like receptor (TLR) was assessed neutralizing antibodies. Histones...
ABSTRACT Background and objective Lipid metabolism dysregulation has been implicated in the pathogenesis of IPF; however, roles most lipid metabolites lung fibrosis remain unexplored. Therefore, we aimed to identify changes tissues IPF patients determine their pulmonary fibrosis. Methods Free fatty acids controls were quantified using a metabolomic approach. The free fibroblasts or epithelial cells treated with TGF‐β1 evaluated fibrotic markers. antifibrotic role stearic acid was also...
Abstract Sarcopenia is an age-related disorder characterised by a progressive decrease in skeletal muscle mass. As the genetic biomarkers for sarcopenia are not yet well characterised, this study aimed to investigate variations related relatively aged cohort, using genome-wide association (GWAS) meta-analyses of lean body mass (LBM) 6961 subjects. Two Korean cohorts were analysed, and subgroup GWAS was conducted appendicular (ASM) index. The effects significant single nucleotide...